A study on the measuremet of DKK-1 in the serum of esophageal cancer patients / 国际肿瘤学杂志

Objective To explore the expression of DKK-1 and its clinical value in patients with e-sophageal cancer. Methods The levels of serum DKK-1 were measured by sandwich enzyme linked immu-nosorbent assay(ELISA) in 80 patients with esophageal squamous cell cancer and in 35 healthy subjects. The sensitivities of serum DKK-1, CEA and CYFRA21-1 in the patients with esophageal squamous cell cancer were calculated and analyzed to compare their values in diagnosis of esophageal cancer. Results The serum DKK- 1 levels in patients with esophageal squamous cell cancer were significantly higher than those of healthy subjects(P<0.05). According to the highest value of medicine reference of DKK- 1 (14. 54 ng/ml), its sensitivity and specificity were 66. 25% and 82. 86% respectively; There was a significant difference between the sensitivity of DKK-1 in patients with esophageal squamons cell cancer(66. 25%) and that in healthy subjects(17.14%) (P<0.05). The sensitivitis of serum DKK- 1 in patients with esophageal squamous cell cancer were associated with tumor size, TNM stage and lymph node metastasis(P <0.05),but not associated with gender,age and differentiation grade(P>0.05). And the sensitivity of DKK-1 (66. 25%) was significantly higher than that of CEA, CYFRA21-1 (39. 44% ,25. 35%, P <0. 01). Conclusion DKK-1 played a very important role in the growth and metastasis of esophageal cancer and may be a new tumor marker or an important index in the diagno-sis of esophageal cancer.

Ozonation of naphthalene sulfonic acids in aqueous solutions: part II--relationships of their COD, TOC removal and the frontier orbital energies.

This paper evaluated the COD, TOC removal and molecular frontier energies in the ozonation of naphthalene sulfonic acids (NSAs). It was found that both COD and TOC degradation of the 11 compounds followed the pseudo-first-order kinetic and the reaction rate constants with ozone varied widely. A linear relationship between logarithm of global COD degradation rate constant and the energy of the highest occupied molecular orbit, E(homo), was obtained for these NSAs with a regression coefficient of 0.88 and a slope of 0.4672 eV(-1). TOC degradation kinetics also reached similar results with a regression coefficient of 0.90 and a slope of 0.9336 eV(-1). The results of correlation analysis suggested that TOC and COD removal in ozonation of NSAs follow the frontier orbital theory and can be predicted by E(homo), which indicated that electrophilic effect was the main factor in the ozonation of NSAs. Therefore, it is possible to improve the biodegradability of dyestuff wastewater bearing mainly NSAs by ozonation at an accepted cost and evaluate the economy of ozonation process.

Ozonation of naphthalene sulfonic acids in aqueous solutions. Part I: elimination of COD, TOC and increase of their biodegradability.

Ozonation of 11 naphthalene sulfonic acids (NSA) in the aqueous solution was studied by bubbling at 31 degrees C at an ozone dose rate of 5.56 mg min(-1) l(-1). COD, TOC and BOD5 of these compounds were tested. It was found that COD and TOC can be removed effectively by ozonation as expected. More than 40% COD of compounds No. I (2-amino-1-NSA), No. 5 (1-hydroxy-7-amino-5-NSA), No. 6 (6-hydroxy-1-NSA), No. 8 (6-amino-1,4-naphthalene disulfonic acid) and No. 11 (I -hydroxy-6-amino-3-NSA) has been eliminated at an ozone dosage of 5.56 mg min(-1) l(-1) for 2 h. Although TOC removal was very different, a good biodegradability was reached for NSAs with an average ozone consumption of 3.0 mgl(-1) for a TOC0 concentration of 100 mg(-1). Ozonation and biotreatment should be good alternatives for these compounds, especially after 20% TOC reduction and TOC removal were more sensitive in predicting an increase in biodegradability during ozonation, than that of COD. NSAs are similar in their behavior with reference to ozone consumption. In order to obtain a good biodegradability of NSAs at a TOC0 concentration of 100 mgl(-1), an ozone consumption between 2.0 and 4.0 mg mg(-1) ACOD is needed for this setup and 3.0 mg O3 mg(-1) TOC0 requirement may be more practical in predicting the biological behavior of naphthalene compounds.

EVALUATION ON DNA EXTRACTION OF ENVIRONMENTAL SAMPLES BY DNA FINGERPRINTS / 微生物学通报

In this paper the application of DNA fingerprints to evaluate the efficiency of DNA extraction was studied DNA mixtures of landfill leachate and active sludg e were extracted using three different methods and the DNA extraction was evalua ted by ARDRA and RISA fingerprints The results showed that RISA is a effecti ve way to evaluate DNA extraction

Application of Its and 16S Ribosomal DNA to Environmental Microbial Ecology / 微生物学通报

A new method was developed for the investigation of microbial ecology, which used the cloning/sequencing technique combined with RISA profiling The method is effective, cheap and easy to use, which can be performed in most of the molecular biological laboratories