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Nosocomial outbreak of varicella zoster virus (VZV) has been reported when susceptible individuals encounter a case of chicken pox or shingles. A suspected VZV outbreak was investigated in a 50-bedded in-patient facility of Physical Medicine and Rehabilitation in a tertiary care multispecialty hospital. A 30-year-old female patient admitted with Pott's spine was clinically diagnosed with chicken pox on 31 December 2022. The following week, four more cases were identified in the same ward. All cases were diagnosed as laboratory-confirmed varicella zoster infection by PCR. Primary case was a housekeeping staff who was clinically diagnosed with chicken pox 3 weeks prior (9 December 2022). He returned to work on eighth day of infection (17 December 2022) after apparent clinical recovery but before the lesions had crusted over. Thirty-one HCWs were identified as contacts a and three had no evidence of immunity. Two of these susceptible HCWs had onset of chickenpox shortly after first dose of VZV vaccination was inoculated. All cases recovered after treatment with no reported complications. VZV infection is highly contagious in healthcare settings with susceptible populations. Prompt identification of cases and implementation of infection prevention and control measures like patient isolation and vaccination are essential for the containment of outbreaks.
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Infecção Hospitalar , Surtos de Doenças , Herpesvirus Humano 3 , Centros de Atenção Terciária , Adulto , Humanos , Varicela/epidemiologia , Infecção Hospitalar/epidemiologia , Infecção Hospitalar/virologia , Herpesvirus Humano 3/isolamento & purificação , Índia/epidemiologia , Assistência de Longa Duração , Infecção pelo Vírus da Varicela-Zoster/epidemiologiaRESUMO
Stability of soil organic carbon (SOC) is pre-requisite for stabilization of C leading to long-term C sequestration. However, development of a comprehensive metric of SOC stability is a major challenge. The objectives for the study were to develop novel SOC stability indices by encompassing physical, chemical, and biochemical SOC stability parameters and identifying the most important indicators from a Mollisol, an Inceptisol, a Vertisol, and an Alfisol under long-term manuring and fertilization. The treatments were control, 100%NPK, 50% NPK+ 50% N through either farmyard manure, cereal residue, or green manure. SOC stability indicators were selected, transformed and integrated into unique SOC stability indices via conceptual framework and principal component analysis. Principal component analysis identified Al-macroaggregate, humic acid C-microaggregate, microaggregate-C, particulate organic matter-C-macroaggregate and polyphenol-microaggregate as the important SOC stability indicators. The principal component analysis -based SOC stability index varied from 0.2 to 0.9, 0.1 to 0.5, 0.2 to 0.6, 0.1 to 0.5 for Mollisol, Inceptisol, Vertisol and Alfisol, respectively. The SOC-stability index derived from conceptual framework and principal component analysis significantly lined up well with one another, although NaOCl-Res-C showed a high correlation with both conceptual framework (r = 0.8) and principal component analysis-based (r = 0.7) SOC stability indexes, suggesting that both methods might be used to quickly assess SOC stability in four soil orders. Overall, 50%NPK+50%N by farmyard manure or green manure emerged as the most effective management practices for enhancing stability of SOC in Mollisol, Inceptisol, Vertisol, and Alfisol of India which might act as major C sink in rice-wheat and maize-wheat cropping systems. The other aspect of C sequestration is to enhance agricultural productivity without depending much on expensive chemical fertilizers. The model yardstick thus developed for assessing SOC stability might be useful to other systems as well.
Assuntos
Carbono , Solo , Solo/química , Carbono/análise , Índia , Fertilizantes/análise , Esterco , Agricultura , Análise de Componente Principal , Nitrogênio/análise , Sequestro de Carbono , Substâncias Húmicas/análiseRESUMO
The full behaviour of natural clay minerals in soil organic carbon (SOC) stabilization in the presence of oxides and external C inputs is yet unknown. Thus, an incubation experiment was conducted in a sand-clay mixture with different soil clay fractions (SCFs) obtained from Alfisol, Inceptisol, Mollisol, and Vertisol in the presence of wheat residues to compare their C stabilization capacity. The C mineralization rates were higher in 1:1 type dominated SCFs (Alfisol and Inceptisol) compared to 2:1 interstratified mineral dominated SCFs (Vertisol). Wheat residues as C source altered SCFs' abilities to stabilize SOC at only moderate dosages of application (3-12 g kg-1). C mineralization and microbial biomass carbon (MBC) fell by 40% and 30%, respectively, as the amount of clay increased from 7.5 to 40%. However, removing sesquioxides from the SCFs boosted C mineralization and MBC by 22% and 16-32%, respectively, which matched with higher enzymatic activities in the sand-clay mixture. The increased C stabilization capacity of Vertisol-SCF may be attributed to its greater specific surface area (SSA) (506 m2 g-1) and cation exchange capacity (CEC) [meq/100 g]. Regression analysis revealed that SSA, CEC, and enzymatic activity explained approximately 86% of total variations in C mineralization. This study highlighted the critical role of 2:1 expanding clay minerals and sesquioxides in greater stabilization of external C input compared to its 1:1 counterpart. It also implied that the role of mineralogy or texture and sesquioxides levels in different soils (Vertisol, Mollisol, Inceptisol, Alfisol) should be prioritized while adding crop residues to reduce C footprint and enhance sequestration.
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Carbono , Solo , Solo/química , Argila , Carbono/análise , Areia , Minerais , Microbiologia do SoloRESUMO
We have examined the electronic structure evolution in transition metal dichalcogenides MX2 where M = Mo, W and X = S, Se, and Te. These are generally referred to as van der Waals materials on the one hand, yet one has band gap changes as large as 0.6 eV with thickness in some instances. This does not seem to be consistent with a description where the dominant interactions are van der Waals interactions. Mapping onto a tight binding model allows us to quantify the electronic structure changes, which are found to be dictated solely by interlayer hopping interactions. Different environments that an atom encounters could change the Madelung potential and therefore the onsite energies. This could happen while going from the monolayer to the bilayer as well as in cases where the stackings are different from what is found in 2H structures. These effects are quantitatively found to be negligible, enabling us to quantify the thickness-dependent electronic structure changes as arising from interlayer interactions alone.
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The formation of a heterostructure with plasmonic nanoparticles drastically alters the optoelectronic properties of graphene quantum dots (GQDs), resulting in exceptional properties. In the present work, we prepare nitrogen-doped GQDs decorated on gold nanoparticles (Au@N-GQDs) by a one-step green reduction method and study its extraordinary fluorescence and photoresponse characteristics. The as-prepared Au@N-GQDs show more than one order of magnitude enhancement in the fluorescence intensity as compared to the bare N-GQDs, which is attributed to hot electron generation and improved absorption in N-GQDs by local field enhancement and the modification of the edge functional groups. Because of the selective coordination to Fe3+ ions, the Au@N-GQDs exhibit extraordinary quenching of fluorescence, with ultrahigh sensitivity for the detection of Fe3+ (<1 nM). A new model for the charge-transfer dynamics is developed involving the Langmuir's law of adsorption to explain the unusual quenching, which strongly deviates from the known models of static/dynamic quenching. The proposed sensor is successfully implemented for the ultrasensitive detection of Fe3+ ions in human serum and Brahmaputra river water samples, representing its high potential applications in clinical as well as environmental diagnosis. Additionally, because of its high absorption in the UV-vis-NIR region and high charge density with long life excitons, the Au@N-GQDs are utilized as photodetectors with â¼104 times faster response than that of bare N-GQDs. The Au@N-GQD-based photodetector possesses a high responsivity of â¼1.36 A/W and a remarkably high external quantum efficiency of â¼292.2%, which is much superior to the GQD-based photodetectors reported till date. The underlying mechanism of ultrafast photoresponse is ascribed to the transfer of hot electrons along with the tunneling of the electrons from Au NPs to N-GQDs as well as the defect reduction of N-GQDs by the incorporation of Au NPs. Without the use of any charge transporting layer, the outstanding performance of N-GQD-based plasmonic photodetector opens up unique opportunities for future high-speed optoelectronic devices.
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Herein, we investigate the origin of excitation wavelength dependent spectral features and high fluorescence quantum yield in fluorescent 2D tungsten disulfide (WS2) quantum dots (QDs) of average size 2.4â¯nm. The as-prepared WS2 QDs possess high optical bandgap and reasonably high fluorescence quantum yield ~15.4% in the green region without any functionalization. The broad photoluminescence (PL) spectrum consists of multiple peaks owing to emissions from excitonic transitions and surface defect-related transitions. The excitation wavelength-dependent spectral redshift and narrowing of line shape in the PL peak are analyzed carefully, and it is attributed to the selective excitation/recombination of carriers from different energy levels. The temperature-dependent PL analysis yields an exciton binding energy of ~301â¯meV in the QDs. Furthermore, we study the interaction between fluorescent WS2 QDs and single-walled carbon nanotubes (SWCNTs) and explore the mechanism of systematic quenching of PL of QDs by SWCNTs. The nature of the Stern-Volmer plot is found to be linear, and the time-resolved fluorescence measurements reveal that the quenching follows primarily the static behavior. Our study further reveals that defect sites in SWCNTs primarily act as the binding sites for WS2 QDs and form non-fluorescent complexes for effective quenching of the PL. The strong interaction between the WS2 QDs and the SWCNTs is evidenced from the spectral shift in the X-ray photoelectron spectroscopy and Raman peaks. Our study reveals the origin of excitation wavelength dependent PL emission from WS2 QDs and the nature of the interaction between WS2 QDs and SWCNTs, which are important for their applications in biomedical imaging and sensing, such as surface-enhanced Raman scattering, etc.
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Majority of organic matter is bound to clay minerals to form stable colloidal organo-mineral fraction (COMF) in soil. Stability of carbon (C) in COMF is crucial for long-term C sequestration in soil. However, information on the effect of long-term fertilization and manuring with various organic sources on C stability in such fraction in soils with varying clay mineralogy is scarce. The present study was, therefore, carried out to assess the effect of thirty-one years of continuous fertilization and manuring with different organics on C-stability in COMF extracted from an Inceptisol, a Vertisol, a Mollisol, and an Alfisol. The treatments comprised of control (no fertilization), 100% NPK (100% of recommended N, P and K through fertilizer), 50% NPK+ 50% of recommended N supplied through either farm yard manure (FYM) or cereal residue (CR) or green manure (GM). The stability of C (1/k) in COMF was determined from desorption rate constant (k) of humus-C by sequential extraction and correlated with extractable amorphous Fe-Al-Si-oxides, and crystallite size of illite minerals. Long-term fertilization and manuring with the above sources of organic altered the contents of amorphous Fe-Al-Si-oxides, and decreased the crystallite size of illite in all the soil orders. Fifty percent substitution of fertilizer N by various organics significantly increased C-stability in COMF by 27-221% (mean 111%) over full dose of NPK (100% NPK). Smectite dominating Vertisol exhibited highest stability of C followed by the Mollisol, the Inceptisol and the Alfisol. Stability of such C in soil was correlated positively with the amount of amorphous Fe and Al oxides but negatively with crystallite size of illite (râ¯=â¯-0.46, Pâ¯<â¯0.01). Application of NPKâ¯+â¯GM or NPKâ¯+â¯FYM in Inceptisol, Vertisol and Mollisol and NPKâ¯+â¯GM or NPKâ¯+â¯CR in Alfisol emerged as the best management practices for higher stabilization of C in COMF for long-term C sequestration.
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Herein, we demonstrated a novel synthetic route to grow size-tunable hybrid perovskite (CH3NH3PbI3 and CH3NH3PbBr3) quantum dots (QDs) using a Fluorine-doped TiO2 (F-TiO2) mesoporous template and these QDs exhibit large exciton binding energy, high photoluminescence quantum yield and improved photostability. The pore size in F-TiO2 template is tuned by varying the HF molar concentration during its solvothermal growth and size of the perovskite QDs embedded in F-TiO2 pores is tuned in the range 1.7-5.1â¯nm, as revealed from the TEM analysis. A systematic blue-shift in UV-visible absorption edge, as well as photoluminescence (PL) spectrum, is observed with the reduced size of the perovskite QDs due to strong quantum confinement. The CH3NH3PbI3 QD with average size â¼1.7â¯nm exhibits â¼47â¯nm blue shift in the PL spectra, â¼43 fold enhancement in PL intensity and â¼25% PL quantum yield (QY). On the other hand, CH3NH3PbBr3 QD of similar size exhibits dramatically enhanced (â¼124 times) PL emission with narrow line width and a PLQY of â¼57%, which is significant for the template-assisted growth of perovskite QDs film. The quantitative analysis of the PL emission energy vs QD size shows an excellent fit with the Brus equation confirming the strong quantum confinement effect in the perovskite QDs. Analysis of low-temperature PL spectra reveals very high exciton binding energy (162-272â¯meV) for the QDs as compared to the bulk film (32â¯meV) due to the high effective dielectric constant, and high electron-hole recombination probability in the QDs, which is consistent with the extremely high PLQY and stable emission from the QDs. The blue shift of the PL peak with increasing temperature is explained on the basis of localization effect. Time-resolved PL analysis for both the perovskite QDs reveals faster life time compared to their bulk counterparts, confirming the significant radiative recombination of carriers in the QDs at the room temperature. The CH3NH3PbBr3 QDs embedded in porous F-TiO2 template maintain its initial PL intensity up to several hours (≥10â¯h) under the UV laser exposure (18mW), while that of the bulk film decreases to <67%. Thus, template grown hybrid perovskite QDs exhibiting high photostability and very high PLQY demonstrated here are promising for the next generation optoelectronic applications.
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We report on the fabrication of Ag nanoparticle (NP) decorated mesoporous Si nanowire (NW) heterostructure (HS) by a simple and low cost chemical process. The as-grown Si NWs are mesoporous in nature and the Ag NP decorated Si NWs (Ag@Si NWs) exhibit broadband light emission, ultralow reflectance, efficient photocatalytic degradation of organic dyes and excellent sensitivity for the detection of organic molecules over a wide range of concentration. The broadband white light photoluminescence emission from the bare Si NWs is explained on the basis of quantum confinement effect in Si NCs/NWs and the nonbridging oxygen hole center defects in the SiSiOx interface. High work function of the noble metal NPs facilitates the effective separation of the photoinduced electron-hole pairs in Si NWs, which enables the Ag@Si NWs to exhibit high photocatalytic efficiency for the degradation of organic dye. The Ag@Si NWs exhibited high potential and sensitivity for the selective and quantitative detection of different organic molecules at extremely low concentration down to 10-12â¯M by surface-enhanced Raman scattering and 10-11â¯M by fluorescence-based detection. These versatile properties of the Ag@Si NWs open up opportunities for a variety of energy and environmental applications, such as white light emission, solar cell, artificial photosynthesis, disposal of organic pollutant and bio-chemical sensors etc.
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We explore the mechanism of the fluorescence enhancement and fluorescence quenching effect of single walled carbon nanotubes (SWCNTs) on highly fluorescent graphene quantum dots (GQDs) over a wide range of concentrations of SWCNTs. At very low concentrations of SWCNTs, the fluorescence intensity of the GQDs is enhanced, while at higher concentrations, systematic quenching of fluorescence is observed. The nature of the Stern-Volmer plot for the latter case was found to be non-linear indicating a combined effect of dynamic and static quenching. The contribution of the dynamic quenching component was assessed through the fluorescence lifetime measurements. The contribution of static quenching is confirmed from the red shift of the fluorescence spectra of the GQDs after addition of SWCNTs. The fluorescence intensity is first enhanced at very low concentration due to improved dispersion and higher absorption by GQDs, while at higher concentration, the fluorescence of GQDs is quenched due to the complex formation and associated reduction of the radiative sites of the GQDs, which is confirmed from time-resolved fluorescence measurements. Laser confocal microscopy imaging provides direct evidence of the enhancement and quenching of fluorescence at low and high concentrations of SWCNTs, respectively. This study provides an important insight into tuning the fluorescence of GQDs and understanding the interaction between GQDs and different CNTs, which is important for bio-imaging and drug delivery applications.
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Grafite/química , Nanotubos de Carbono/química , Pontos Quânticos/química , Microscopia Confocal , Espectrofotometria , Análise Espectral RamanRESUMO
The present study evaluates the protective potential of the flavonoid naringenin (NRG) against experimentally induced cadmium (Cd) toxicity in Swiss albino mice. NRG (4 and 8 mg/kg) was orally administered to mice 30 min before oral administration of CdCl2 (12 mg/kg) for 11 consecutive days. On the 12th day, we evaluated body and organ weights, hematological profiles, serum biochemical profiles, and hepatic and renal tissue antioxidative parameters including lipid peroxidation, reduced and oxidized glutathione, glutathione-S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase, and catalase. Cotreatment with NRG markedly and significantly normalized body and organ weights, hematological profiles, and serum biochemical profiles and significantly modulated all of the hepatic and renal tissue biochemical parameters in Cd-intoxicated mice. The present findings show that NRG possesses a remarkable alleviative effect against Cd-induced toxicity in albino mice, mediated by abrogation of Cd-induced oxidative stress by multiple mechanisms.
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Antioxidantes/farmacologia , Cloreto de Cádmio/toxicidade , Flavanonas/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Animais , Análise Química do Sangue , Peso Corporal/efeitos dos fármacos , Relação Dose-Resposta a Droga , Testes Hematológicos , Masculino , Camundongos , Tamanho do Órgão/efeitos dos fármacosRESUMO
The present study evaluated the ameliorative potential of ß-carotene (BCT) against experimentally induced arsenic toxicity in Swiss albino mice. BCT (5 and 10 mg/kg) was administered orally to mice 30 min before oral administration of arsenic trioxide (3 mg/kg) for 14 consecutive days. On 15th day, the body weights, organ weights, hematological profiles, serum biochemical profile; hepatic and renal antioxidative parameters viz. lipid peroxidation, reduced and oxidized glutathione, glutathione-S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase; and DNA fragmentation were evaluated. Co-treatment with BCT markedly and significantly normalized body weights, organ weights, hematological profiles, serum biochemical profile and significantly modulated all the hepatic and renal biochemical parameters and DNA fragmentation in arsenic-intoxicated mice. The present findings conclude that ß-carotene possessed remarkable ameliorative effect against arsenic-induced toxicity in albino mice mediated by its antioxidant and antigenotoxic properties.
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Peso Corporal/efeitos dos fármacos , Fígado/efeitos dos fármacos , Óxidos/toxicidade , beta Caroteno/farmacologia , Alanina Transaminase/sangue , Animais , Antineoplásicos/administração & dosagem , Antineoplásicos/toxicidade , Trióxido de Arsênio , Arsenicais/administração & dosagem , Aspartato Aminotransferases/sangue , Contagem de Células Sanguíneas , Catalase/metabolismo , Fragmentação do DNA/efeitos dos fármacos , Glutationa/metabolismo , Dissulfeto de Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Glutationa Redutase/metabolismo , Glutationa Transferase/metabolismo , Fígado/metabolismo , Fígado/patologia , Masculino , Camundongos , Tamanho do Órgão/efeitos dos fármacos , Óxidos/administração & dosagem , Superóxido Dismutase/metabolismo , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Fatores de Tempo , Vitaminas/administração & dosagem , Vitaminas/farmacologia , beta Caroteno/administração & dosagemRESUMO
In the present study, we evaluated the ameliorative potential of a citrus flavonoid, naringenin (NRG), against experimentally induced arsenic toxicity in Swiss albino mice. NRG (5 and 10 mg kg-l) was administered orally to mice 30 minutes before oral administration of arsenic trioxide (3 mg kg-l) for 14 consecutive days. On day 15, the following parameters were evaluated: body weight; organ weight; hematological profile; serum biochemical profile; hepatic and renal tissue antioxidative parameters including lipid peroxidation, reduced and oxidized glutathione, glutathione-S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase levels; and DNA fragmentation. Co-treatment with NRG markedly and significantly normalized body weights, organ weights, hematological profiles, and serum biochemical profiles and significantly modulated all of the hepatic and renal tissue biochemical parameters and DNA fragmentation in arsenic-intoxicated mice. The present findings indicate that naringenin remarkably ameliorated the effects of arsenic-induced toxicity in albino mice due to its strong antioxidant property.
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Antioxidantes/farmacologia , Flavanonas/farmacologia , Óxidos/toxicidade , Animais , Trióxido de Arsênio , Arsenicais , Análise Química do Sangue , Peso Corporal/efeitos dos fármacos , Fragmentação do DNA/efeitos dos fármacos , Relação Dose-Resposta a Droga , Testes Hematológicos , Masculino , Camundongos , Tamanho do Órgão/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacosRESUMO
Mn doping in ZnS nanoplatelets has been shown to induce a structural transition from the wurtzite to the zinc blende phase. We trace the origin of this transition to quantum confinement effects, which shift the valence band maximum of the wurtzite and zinc blende polymorphs of ZnS at different rates as a function of the nanocrystal size, arising from different effective hole masses in the two structures. This modifies the covalency associated with Mn incorporation and is reflected in the size-dependent binding energy difference for the two structures.
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BACKGROUND: Almost 30% of chronic haemodialysis (HD) patients are dependent on central venous catheters (CVCs) for their vascular access, and catheter-related bacteraemia (CRB) is the major reason for catheter loss and has been associated with substantial morbidity, including meta-static infections. This systematic review evaluates the benefits and harms of antimicrobial interventions for the prevention of catheter-related infections (CRIs). METHODS: MEDLINE (1950-May 2009), EMBASE (1980-May 2009) CENTRAL (up to May 2009) and bibliographies of retrieved articles were searched for relevant RCTs. Analysis was by a random effects model and results expressed as rate ratio, relative risk (RR) and weighted mean difference (WMD) with 95% confidence intervals (CI). RESULTS: A total of 29 trials with 2886 patients and 3005 catheters were included. Antimicrobial catheter locks (AMLs) significantly reduced the rates of CRBs (rate ratio, 0.33, 95% CI 0.24-0.45) and exit-site infections (ESIs) (rate ratio 0.67, 95% CI 0.47-0.96). Exit-site antimicrobial application also significantly reduced the rates of CRBs (rate ratio 0.21, 95% CI 0.12-0.36) and ESIs (rate ratio 0.22, 95% CI 0.10-0.47). Antimicrobial coating of HD catheters and the use of peri-operative antimicrobials did not result in significant reduction in rates of CRBs and ESIs. CONCLUSION: The use of AMLs and exit-site antimicrobials are useful measures in the reduction of CRIs, whereas antimicrobial impregnated catheters and peri-operative systemic antimicrobial administration have not been found to be beneficial. Further head-to-head trials of various AMLs and exit-site antimicrobials are needed to know about their comparative clinical efficacy.
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Anti-Infecciosos/uso terapêutico , Infecções Relacionadas a Cateter/prevenção & controle , Diálise Renal , Ensaios Clínicos como Assunto , HumanosRESUMO
Fungal peritonitis is a significant cause of morbidity and mortality in patients undergoing peritoneal dialysis (PD). This retrospective study evaluated patients with fungal peritonitis in our dialysis unit from December 1999 through September 2003. We analyzed the demographic and clinical characteristics of patients to identify possible risk factors. Main clinical outcomes of interest were technique survival and mortality. During the study period, 18 patients were diagnosed with fungal peritonitis (15 on continuous ambulatory PD, 3 on automated PD). Fungal peritonitis accounted for 6% of all peritonitis episodes during the study period. Candida species were the infecting organism in 15 patients (83%). All 18 patients received antifungal treatment according to the International Society of Peritoneal Dialysis (ISPD) recommendations that were current during the study period. Two patients (11%) died with the PD catheter in situ, and 1 patient died after dialysis was withdrawn because of deterioration in other comorbid conditions. All the surviving patients were switched to hemodialysis. Of the 18 patients, 15 (83%) had history of antibiotic treatment for bacterial peritonitis within the 4 weeks preceding the fungal peritonitis episode. Our study provides further support for the current ISPD recommendation that the PD catheter should be removed as soon as a diagnosis of fungal peritonitis is made in a patient.
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Micoses/etiologia , Diálise Peritoneal/efeitos adversos , Peritonite/etiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Micoses/diagnóstico , Micoses/microbiologia , Diálise Peritoneal Ambulatorial Contínua/efeitos adversos , Peritonite/diagnóstico , Peritonite/microbiologia , Fatores de RiscoRESUMO
We have cloned and sequenced a DNA fragment (approximately 1 kb) containing a complete open reading frame from a cDNA library of Leishmania donovani promastigotes. The alignment of the derived polypeptide sequence and the modeling studies revealed that the protein is highly homologous to the mammalian cyclins having conserved cyclin box and substrate-docking motif. Northern blot analysis of the RNA isolated from synchronized L. donovani promastigotes showed periodic expression of the message with maximum abundance at S-phase suggesting its involvement in the events related to the regulation of DNA replication. The results confirm that we have isolated a cyclin molecule from L. donovani (LdCyc1) which may play an important role in the regulation of the parasite cell cycle.