Hydroalcoholic root extracts of Houttuynia cordata (Thunb.) standardized by UPLC-Q-TOF-MS/MS promotes apoptosis in human hepatocarcinoma cell HepG2 via GSK-3ß/ß-catenin/PDL-1 axis.

Houttuynia cordata (Thunb.), an important medicinal plant of Northeast India, Korea, and China, is used to treat various ailments and for anticancer research. Knowing its traditional practices, we are interested in the mode-of-action of HCT on HepG2 to co-relate the traditional practice with modern drug therapeutics. UPLC-Q-ToF-Ms analysis of HCT reveals identification of 14 metabolites. Network pharmacology analysis of the 14 compounds showed interaction with 232 different targets with their potential involvement in hepatocellular carcinoma. Whole extracts impart cytotoxicity on variety of cell lines including HepG2. There was a significant morphological alteration in treated HepG2 cells due to impairment of cytoskeletal components like ß and γ- tubulin. Arrest at G1-S checkpoint was clearly indicated downregulation of Cyclin D1. The root extracts actuated apoptosis in HepG2 as evident from altered mitochondrial membrane potential, Annexin V- FITC, BrdU-PI, AO/EtBr assays, and modulations of apoptotic protein expression but without ROS generation. Whole extracts caused abrogation of epithelial to mesenchymal transition with repression of Snail, N-Cadherin, Vimentin, MMP-9, and upregulation of Pan-Cadherin. Pathway analysis found GSK-3ß in Wnt/ß-Catenin signaling cascade to be involved through Hepatocellular carcinoma (hsa05225) pathway. The GSK-3ß/ß-Catenin/PDL-1 signaling was found to be inhibited with the downregulation of pathway components. This was further confirmed by application of EGF, an inducer of the GSK-3ß/ß-Catenin pathway that neutralized the effect of Houttuynia cordata (Thunb.) root extract on the said pathway. Network pharmacology analysis also confirms the synergy network with botanical-bioactive-target-disease which showed Kaempferol to have the highest degree of association with the said pathway.

Oral Health Status and Treatment Needs of Chromium Mine Workers in India.

Background: Several diseases are related to occupation. The workers in chromium mines may be exposed to hazardous environments. The objective of this research was to evaluate the oral health condition and treatment needs of chromium mine workers. Materials and Methods: This was a cross-sectional study conducted on workers in the chromium mines located in the Jajpur district, Odisha. The study included a total of 453 mine workers. The World Health Organization (WHO) oral health assessment proforma (1997) was used to assess the oral health status and treatment needs of the workers. Face-to-face interviews were conducted to acquire information on socio-demographic data, along with clinical examinations of type III. Frequency distribution analysis and independent-sample t-test were performed. Results: The majority of mineworkers demonstrated poor oral health status with high caries experience (Decayed Missing and Filled Tooth (DMFT) = 3.13 ± 1.82). The prevalence of tobacco consumption was very prevalent among them (74.8%). Leukoplakia (13.2%) was the most commonly noted oromucosal lesions. The buccal mucosa (16.8%) and commissures of the lips (3.3%) were the most common affected sites. Most workers demonstrated poor periodontal conditions. More than half of the mine workers (53.6%) showed malocclusion. Restoration, extraction, and pulp care were needed in most of the workers. Conclusion: A critical intervention should be provided to promote oral hygiene among Indian chromium mine workers. Dental health education and tobacco cessation programs are of utmost importance to improve the health conditions of these workers.

Therapeutic importance of Cucurbitaceae: A medicinally important family.

ETHNOPHARMACOLOGICAL RELEVANCE: Medicinal plants of Cucurbitaceae family consist of several edible fruits and vegetables consumed worldwide since ancient times. The plants of this family have played an essential role in the ethnopharmacological as well as traditional medicinal system globally and their evidence is well established in several traditional literatures. Various plant parts have been used to treat several human ailments viz. Pandu (anemia), Pliharoga (splenomegaly), Sopha (inflammation), Gulma (tumor growth), Adhmana (indigestion. acidity), Garavisa (poisoning) etc. AIM OF THE REVIEW: This review article aims to systematically document and bridge scientific evidences with the ethnopharmacological, ethnoveterinary and folklore claims along with the therapeutic efficacy with mechanism of action found in different literature, books, and scientific articles belonging to the Cucurbitaceae family. MATERIALS AND METHODS: To construct the manuscript a comprehensive literature review was done based on the information collected from Ayurvedic Pharmacopoeia of India; books, research articles and databases such as ScienceDirect, Wiley Online Library, SciFinder, Scopus, Springer, Google Scholar, Web of Science, ACS Publications and PubMed. RESULTS: The plants of Cucurbitaceae family are rich in phytochemicals like terpenoids, glycosides, alkaloids, saponins, tannins, steroids, etc., responsible for the therapeutic effect. Various parts of these plants such as leaves, stems, flowers, fruits, seeds, roots etc. exhibit a plethora of pharmacological activity viz. hypolipidemic, antihyperglycemic, anticancer, antimicrobial, analgesic, anti-inflammatory, anti-stress and immunomodulatory activities. Also, in-vitro and in-vivo reports suggest strong inhibitory potential against α-glucosidase, α-amylase, lipase, carbonic anhydrase enzyme along with antioxidant, anti-inflammatory, antidiabetic, anti-tumor, antifungal, etc. Furthermore many reports suggest these plants are beneficial for nutritional, economical and ethnoveterinary uses. CONCLUSIONS: The current review enlightens the therapeutic potential of the gourd family, comprising of the geographical origins, morphology, phytochemistry, ethnopharmacology, ethnoveterinary, nutritional importance, therapeutic benefits, safety, efficacy and related aspects. The phytochemical and pharmacological potential indicated will popularize this family as a potential source of novel therapeutic agents and functional foods. This study will help to validate the therapeutic claims of several ethnomedicinal uses of this plant family. Furthermore the Cucurbitaceae family needs to be evaluated based on the combine approaches of chemoprofiling and bioexploration to develop the concept of food as medicine for the development of new generation therapeutics leading to the human wellness.

Comparison of two non-radiographic techniques of mixed dentition space analysis and evaluation of their reliability for Bengali population.

CONTEXT: Mixed dentition arch analysis system is an important criterion in determining the type of orthodontic treatment plan. Different mixed dentition arch analysis system are available and among them both Moyer's and Tanaka-Jhonson method of space analysis was developed for North American children. Anthropological study reveals that tooth size varies among different ethnicities The present study was performed to determine the reliability of Moyer's and Tanaka-Jhonson's method of mixed dentition arch analysis system among Bengali population. AIMS: To perform the comparative evaluation of the two mixed dentition space analysis system among Bengali population. MATERIALS AND METHODS: Dental casts of maxillary and mandibular arches of 70 Bengali children with permanent dentitions were fabricated. The mesiodistal crown dimensions of all erupted permanent incisors, canines, and premolars were measured with digital callipers. For particular sum of mandibular incisors, Moyer's and Tanaka-Jhonson's mixed dentition arch analysis were calculated and further statistical analysis was carried on. STATISTICAL ANALYSIS USED: Descriptive statistics including the mean, standard deviation, and minimum and maximum values, unpaired't' tests, correlation coefficient "r" were calculated and tabulated. RESULTS: Tanaka and Johnston regression equations under-estimated the mesiodistal widths of permanent canines and premolars. On the other hand, there were no statistically significant differences between actual mesiodistal widths of canines and premolars and the predicted widths from Moyers charts at the 50% level for the lower and upper arches, among Bengali population. CONCLUSIONS: The study suggested that both Moyer's and Tanaka-Jhonson's mixed dentition arch analysis are applicable in Bengali population but with little modification in their regression equation.