Draft genome sequence of the silver pomfret fish, Pampus argenteus.

Silver pomfret, Pampus argenteus, is a fish species from coastal waters. Despite its high commercial value, this edible fish has not been sequenced. Hence, its genetic and genomic studies have been limited. We report the first draft genome sequence of the silver pomfret obtained using a Next Generation Sequencing (NGS) technology. We assembled 38.7 Gb of nucleotides into scaffolds of 350 Mb with N50 of about 1.5 kb, using high quality paired end reads. These scaffolds represent 63.7% of the estimated silver pomfret genome length. The newly sequenced and assembled genome has 11.06% repetitive DNA regions, and this percentage is comparable to that of the tilapia genome. The genome analysis predicted 16 322 genes. About 91% of these genes showed homology with known proteins. Many gene clusters were annotated to protein and fatty-acid metabolism pathways that may be important in the context of the meat texture and immune system developmental processes. The reference genome can pave the way for the identification of many other genomic features that could improve breeding and population-management strategies, and it can also help characterize the genetic diversity of P. argenteus.

Intralesional injection treatment of hypertrophic scars and keloids: a systematic review regarding outcomes.

BACKGROUND: The aim of this review was to explore the existing body of literature focusing on the intralesional treatments of keloids and hypertrophic scars. METHODS: A comprehensive systematic review of related articles was conducted across multiple databases. Article selection was limited to those published in the English language between 1950 and 2014. Search terms for the on-line research were "scar(s)," "keloid(s)," "hypertrophic," "injection," "intralesional," and "treatment". RESULTS: The initial search returned 2548 published articles. After full text review, the final search yielded 11 articles that met inclusion criteria. A total of 14 patient samples in 11 articles were collected. The most frequent intralesional injection treatment studied was triamcinolone (n = 5), followed by bleomycin (n = 3), 5-fluorouracil (n = 2), verapamil (n = 2), cryosurgery, and collagenase. The scar height reduction for all but one study was demonstrated, with acceptable complication and recurrence rate. Only three articles reported a follow-up period longer than 18 months, and only two studies used standardized outcome criteria with a quantitative scale. CONCLUSIONS: Although many treatment options have already been described in the literature, there is no universally accepted treatment resulting in permanent hypertrophic or keloid scar ablation. The lack of adequately long-term powered randomized controlled trials does not permit to establish definitive conclusions with implications for routine clinical practice. LEVEL OF EVIDENCE: III/Therapeutic.

Augmentation mammoplasty/mastopexy: lessons learned from 107 aesthetic cases.

BACKGROUND: One-stage augmentation/mastopexy entails the challenge of augmenting breast volume with an implant while resecting excess skin. Although both augmenting and lifting the breast in a one-stage operation is gaining in popularity, its safety and its efficacy are still under debate and merit deeper evaluation. METHODS: We retrospectively reviewed our experience over a 5-years period with patients who underwent augmentation mammoplasty/mastopexy with the specific objectives of documenting their outcomes and formulating algorithms for safe, simple, and effective operative strategies to manage such patients. Our surgical approach to augmentation/mastopexy breast ptosis was described step by step. Patient satisfaction with the results was also evaluated. RESULTS: One hundred seven patients underwent successful simultaneous augmentation/mastopexy surgery. Sixty-nine underwent primary breast surgery and 38 underwent secondary breast surgery. In 12 cases a periareolar mastopexy scar was used, while in 51 patients a vertical approach was preferred; in 11 and 33 patients a short "T" and an inverted "T" scar mastopexy was necessary, respectively. Few complications were observed, with a very low overall complication rate (14 %) and a reoperation rate of 12.1 % at 14.7 months. Patient satisfaction with the results of this procedure was extremely high. CONCLUSIONS: Simultaneous augmentation/mastopexy is an effective and versatile way to lift the NAC, tighten the breast skin, increase breast projection, and fill in the upper pole. Our technique of simultaneous breast mastopexy after augmentation through a lower periareolar approach showed excellent correction of pre-existing ptosis, making this method highly reliable because the intraoperative tailor-tacking was customized to the patient, implant size, and projection.

The thin gluteal artery perforator free flap to resurface the posterior aspect of the leg and foot.

BACKGROUND: The authors evaluated the clinical application of the gluteal artery perforator free flap harvested above the superficial fascia as a new approach to reconstruct soft-tissue defects of the posterior aspect of the lower limb. METHODS: Between September of 2010 and August of 2013, 27 thin flaps were used to reconstruct lower extremity defects in 27 patients. All flaps were elevated on the superficial fascia plane and based on a single perforator from either the superior or inferior gluteal artery. RESULTS: With the exception of two flaps that healed secondarily after partial loss, all flaps survived completely. Average flap thickness was 8.5 mm (range, 5 to 11 mm). The average flap size was 125 cm (range, 9 × 4 cm to 25 × 12 cm). The average pedicle length was 5.5 cm (range, 3 to 8 cm.). The average artery diameter was 0.65 mm (range, 0.4 to 1.3 mm). During the average follow-up of 13 months (range, 2 to 35 months), all flaps showed good contour and did not require any secondary revisions. CONCLUSIONS: The gluteal artery perforator free flap provides a thin flap for ideal contour, minimizes the operative risks of changing the patient's position intraoperatively, and conceals the donor site with minimal morbidity. However, the pedicle can be short and supermicrosurgery skills may be needed to accommodate the short pedicle. Overall, this new approach of using a thin gluteal artery perforator free flap to reconstruct the posterior aspect of the leg and foot can be considered a feasible option. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, IV.

Indications, outcomes, and complications of pedicled propeller perforator flaps for upper body defects: a systematic review.

BACKGROUND: The aim of this investigation was to systematically review the current literature to provide the best data for indications, outcomes, survival, and complication rates of pedicled propeller perforator flaps for upper body defects. METHODS: A comprehensive literature review for articles published from January 1991 to December 2011 was performed using the PubMed, Medline, and Cochrane Databases. Articles without available full-text, single case reports or papers with excessive missing data were excluded. Papers reporting pedicle-perforator (propeller) flaps used for lower extremity reconstruction were excluded from meta-analysis. RESULTS: From the initial 1,736 studies our search yielded, 343 studies qualified for the second stage of selection. Of 117 full-text reports screened, 41 studies, met the definitive inclusion and exclusion criteria. Of the selected 41 articles, 26 were case series, original papers or retrospective reviews and were included, whereas 15 were case report papers and therefore were excluded. Two hundred ninety-five propeller flaps were reported to have been used in a total of 283 patients. Indications include repair of trauma-induced injuries, post-trauma revision surgery, cancer resection, chronic infection, pressure sores, and chronic ulcers with a major complication rate (3.3%) comparable to that of free flaps. No specific exclusion criteria for the procedure were presented in the studies reviewed. CONCLUSIONS: Pedicled propeller flaps are a versatile and safe reconstructive option that are easy and quick to raise and that provide unlimited clinical solutions because of the theoretical possibility of harvesting them based on any perforator chosen among those classified in the body.