Effects of black pepper and turmeric powder on growth performance, gut health, meat quality, and fatty acid profile of Japanese quail.

In poultry production, the search for alternatives to in-feed antibiotics continues unabated. This study investigated the effects of dietary supplementation of black pepper and turmeric powder, separately or in combination, on the growth performance, gastrointestinal microbiota population, intestinal morphology, serum biochemical parameters, meat quality, and meat fatty acid profile in Japanese quails. Five hundred-day-old mixed-sex Japanese quail chicks were randomly assigned to one of five treatments: a control diet (CON); CON +0.2% antibiotic flavomycin as an antibiotic growth promoter (AGP); CON +0.5% turmeric powder (TUP); CON +0.5% black pepper powder (BPP); and CON +0.5% TUP, and 0.5% BPP (MIX). The findings showed that quail chicks fed AGP and TUP throughout the rearing period had better body weight gain (p = 0.007) and feed conversion ratio (p = 0.02) than the other treatments. The TUP, BPP, and MIX feeds reduced (p = 0.005) abdominal fat percentage. The MIX group had a better breast muscle water-holding capacity (p = 0.04) and lightness index (p = 0.02) and lower (p = 0.02) malondialdehyde concentration after 7 days of refrigerated storage. Feeding BPP, TUP, and MIX diets decreased (p = 0.001) serum cholesterol concentration. Quail chicks fed the CON diet showed significantly higher coliform counts in the crop and ileum (p < 0.001), whereas the lactic acid bacterial population was lower (p = 0.008) in the ileum. Birds that received the MIX diet exhibited a higher (p = 0.02) villus height to crypt depth ratio in the duodenum compared to the other groups. The tested feed additives increased (p < 0.001) villus height in the jejunum and ileum compared to other groups. Feeding the TUP, BPP, and MIX diets reduced (p < 0.001) total saturated fatty acid content and increased (p = 0.004) total polyunsaturated fatty acid concentration, where the MIX diet had the best results. Overall, the present data indicate that supplementing the basal diet with turmeric powder enhances the growth performance of Japanese quails. In some respects, such as gut health and meat quality, combining turmeric powder and black pepper powder was more effective than using them independently.

The effect of fermented rapeseed meal on production performance, egg quality and hatchability in broiler breeders after peak production.

1. This experiment was conducted to evaluate the effects of replacing soybean meal (SBM) with different levels of rapeseed meal (RSM) or fermented rapeseed meal (FRSM) on performance, egg quality and hatchability in broiler breeders.2. RSM was fermented with Bacillus subtilis and Aspergillus niger for 25 d. A total of 140 Ross 308 broiler breeder hens (52 weeks old; 20 per treatment, four hens per cage replicate) were assigned to seven dietary treatments in a completely randomised design involving a control (maize-SBM diet) and a 2 × 3 factorial arrangement, consisting of two types of meal (RSM and FRSM) and three replacement levels (33%, 66% or 100%) for SBM, with one cockerel housed within each cage replicate for 12 weeks.3. Fermentation increased the population of lactic acid bacteria and crude protein and decreased pH, dry matter, crude fibre and anti-nutrients in FRSM meal compared to RSM (P < 0.05).4. Body weight gain and egg weight were not affected by RSM or FRSM. Feeding FRSM compared to RSM increased egg production and egg mass (P < 0.05). FRSM or RSM at the level of 100% significantly decreased egg production and egg mass compared to the control group (P < 0.05). Neither types of meal nor levels of replacing significantly affected egg quality variables except eggshell strength. Yolk colour in all experimental treatments was significantly higher than the control group (P < 0.05). By increasing the level of RSM or FRSM in the diet, hatching rate and chicken weight decreased (P < 0.05).5. Microbial fermentation improved the nutritional value of RSM which could be used to replace SBM with RSM or FRSM at levels lesser than 66% as a suitable protein alternative for broiler breeders.

The Effects of Nano and Micro Particle Size of Zinc Oxide on Performance, Fertility, Hatchability, and Egg Quality Characteristics in Laying Japanese Quail.

The current trial was conducted by using the 288 Japanese quails to evaluate the effects of a zinc-deficient diet supplemented with nano and micro zinc oxide on performance, fertility, hatchability, and egg quality characteristics. In this experiment, birds were randomly allocated to 9 dietary treatments includes diets supplemented with nano or micro particles of zinc oxide (amount of ZnO supplement for each treatment) to supply 49, 74, 99, and 124 mg zinc per kilogram of diet in a factorial arrangement (2 × 4) and a control non supplemented diet (24 mg/kg) with four replicates of eight birds (six females and two males) in each pen. Birds were fed the experimental diets from 47 to 75 days of age and had free access to water and feed during the experimental period. Results showed that Zn supplementation, regardless of particle size, improved the eggshell thickness (P < 0.01). A significant (P < 0.05) interaction was observed between zinc level and ZnO particle size for Shell breaking strength. Quails fed diets supplement with ZnO showed significantly higher egg weight and eggshell surface (P < 0.05) as compared with birds fed a non-supplemented control diet. Results obtained here showed that supplementation of nano ZnO enhanced fertility considerably. Application of non-linear quadratic models showed that the maximum egg production percentage was achieved when 67 or 72 mg/kg of dietary zinc was supplied from nano and micro ZnO, respectively. This result indicated that nano ZnO could reduce the zinc requirement in laying Japanese quail.

Enhancement of Productive Performance, Bone Physical Characteristics, and Mineralization of Laying Hens during the Post-Peak Period by Genistein.

Genistein (GEN), a soybean isoflavone, is structurally and functionally similar to endogenous estrogen; therefore, it has the potential to enhance estradiol properties. This study aimed to evaluate the effects of GEN on the reproductive performance and bone status of laying hens. In total, 80 Hy-line W-36 (40 weeks old, the late stage of egg production cycle) with an initial body weight of 1,230&plusmn;15.8 g (Mean&plusmn;S.E.M), similar egg production, and egg weight were randomly assigned into two groups with 10 replicates and 4 birds in each replicate (40 laying hens per group). Laying hen diets had 0 (control) and 20 mg/kg GEN (white powder, Sichuan Guanghan co. Ltd., purity of 98.5%) for 6 weeks (41 to 46). At the end of the experiment, 20 hens (one hen from each replicate) were slaughtered, and the samples of bone and shell gland (approximately 50 mg) were surgically taken immediately after slaughter for Real-time PCR. The results indicated that dietary GEN increased egg production, feed intake, and egg mass; however, it decreased egg weight (p &lt;0.05). Furthermore, the feed conversion ratio was greater in birds received GEN, compared to those in the control group (p &lt;0.05). GEN enhanced egg quality indices included eggshell strength, thickness, and percentage (p &lt;0.05). Mechanical properties of the tibia, such as weight, length, and breaking strength were also increased by GEN (p &lt;0.05). Moreover, dietary GEN increased the calcium content of the tibia (p &lt;0.05). The mRNA expression of Calbindin-D28k (CaBP-D28k) and transient receptor potential vanilloid channel type 6 (TRPV6) upregulated in eggshell glands of hens treated with GEN paralleled to the controls (p &lt;0.05). In conclusion, the findings of the present study showed that GEN had the potential to improve the bone physical characteristics, mineralization, and the productive performance of Hy-line W-36 laying hens in their post-peak period.

Supplementing flaxseed oil for long periods improved carcass quality and breast fatty acid profile in Japanese quail.

The efficient time for supplementing flaxseed oil to meat-type quail to produce n-3 fatty acid fortified meat has not been determined. This study was conducted to find out the effects of different periods of flaxseed oil supplementation in the Japanese quail diet on the subsequent growth performance, carcass characteristics, fatty acids profile of breast, and functional properties of the Japanese quail meat. Totally, 720 one-day-old Japanese quail were studied in a 35-day experiment using a completely randomized design. Substituting sunflower oil with flaxseed oil had no significant effect on weight gain and feed intake in Japanese quails. Supplementing flaxseed oil for the whole 35-day growth period significantly reduced abdominal fat proportion. Flaxseed oil addition to the quail diet just a week before slaughter resulted in a 4.97-fold increase in the n-3 fatty acid content of the breast muscle. Feeding flaxseed oil decreased the activity of delta-9-desaturase in quail's breast compared to sunflower oil. The greatest thrombogenic index observed in the breast meat from control while consumption of flaxseed oil significantly decreased the thrombogenic index. Supplementing flaxseed oil to quail's diet for 21 and 35 days before slaughter significantly increased breast malondialdehyde content. Feeding flaxseed oil for long periods had no significant impact on the breast meat pH while water holding capacity was decreased. The breast lightness was increased when the flaxseed oil was fed for longer periods. Generally, feeding flaxseed oil in the last week of the growth period improved the feed conversion ratio and the fatty acid profile of quail breast meat with the minimum deterioration effects on meat quality characteristics.

Effects of Pediococcus acidilactici, mannan-oligosaccharide, butyric acid and their combination on growth performance and intestinal health in young broiler chickens challenged with Salmonella Typhimurium.

This study compared the efficacy of Pediococcus acidilactici, mannan-oligosaccharide, butyric acid, and their combination on growth performance and intestinal health in broiler chickens challenged with S. Typhimurium. Ross 308 male broilers (n = 420) were randomly assigned to one of the 6 treatments, resulting in 5 replicate pens of 14 chicks per treatment. The treatments included a negative control [(NC), no additive, not challenged]; positive control [(PC), no additive, but challenged with S. Typhimurium at d 3 posthatch], and 4 groups whereby birds were challenged with S. Typhimurium at d 3 posthatch and fed diets supplemented with either probiotic [0.1 g/kg Pediococcus acidilactici (PA)], prebiotic [2 g/kg mannan-oligosaccharides (MOS)], organic acid [0.5 g/kg butyric acid (BA)], or a combination of the 3 additives (MA). The S. Typhimurium challenge decreased feed intake, body weight gain and increased feed conversion ratio and reduced jejunum villus height (VH) and VH to crypt depth (CD) ratio (P < 0.05). Birds on the MA treatment exhibited similar performance to birds on the NC treatment (P > 0.05) and had a lower population of Salmonella in the ceca compared with birds on the PC treatment, at d 14 and 21 post-challenge (P < 0.05). The lowest heterophil to lymphocyte ratio was observed in birds on the MA and NC treatments (P < 0.05). Birds fed diets supplemented with MA or PA had greater VH and VH: CD ratio than birds on the PC treatment at d 7, 14 and 21 d post-challenge (P < 0.05). Suppressed amylase and protease activity was observed as a result of the S. Typhimurium challenge; the enzyme levels were restored in birds fed the additive-supplemented diets, when compared to the birds on the PC treatment, particularly at d 21 post-challenge (P < 0.05). These results indicate that dietary supplementation with a combination of PA, BA, and MOS in broiler chickens could be used as an effective tool for controlling S. Typhimurium and promoting growth performance.

Effects of fermented cottonseed meal on the growth performance, gastrointestinal microflora population and small intestinal morphology in broiler chickens.

1. This experiment was conducted to evaluate the effects of replacing dietary cottonseed meal (CSM) or fermented cottonseed meal (FCSM) for soya bean meal (SBM) on growth performance, carcass characteristics, gastrointestinal microbial populations, and intestinal morphology of broiler chickens. 2. CSM was fermented with Bacillus subtilis, Aspergillus niger and A. oryzae for 7 d. A total of 300 one-d-old male Ross 308 broiler chickens were used in a 42-d experiment in which the birds were randomly allotted to one of 5 dietary treatments (containing 0%, 10% and 20% CSM or FCSM) in a completely randomised design. Birds were reared on litter floor and had free access to feed and water during the experiment. 3. Results indicated that the fermentation process significantly reduced crude fibre and free gossypol, while it increased crude protein content and lactic acid bacteria (LAB) count in CSM. 4. The use of FCSM instead of CSM significantly improved growth performance of broilers. The abdominal fat yield in treatments containing FCSM was significantly lower than in the other treatments. The increase in the population of LAB in the crop and decrease in the population of coliforms in the ileum of birds fed on diets containing FCSM were more significant than in other birds. Villi in the duodenum and jejunum of the birds fed on diets containing FCSM were significantly higher than for the other experimental groups. 5. The positive effects of diets containing FCSM on growth performance and intestinal health of broiler chickens showed that this processed source of protein can serve as an appropriate alternative for SBM in diets for broiler chickens.

Study on the chemical and microbial composition and probiotic characteristics of dominant lactic acid bacteria in fermented poultry slaughterhouse waste.

This study aimed to evaluate the quality of fermented poultry slaughterhouse waste and isolate and identify the probiotic bacteria present in it. Poultry slaughterhouse waste samples supplemented with 5% starter culture and 0.2% propionic acid received one of four experimental treatments, which consisted of further supplementation with: (1) 10% molasses (M10); (2) 15% molasses (M15); (3) 10% molasses with 200ppm butylated hydroxytoluene (MB10); and (4) 15% molasses with 200ppm butylated hydroxytoluene (MB15). All experimental treatments (silages) were fermented for 6days with sampling before fermentation (day 1) and at the end of days 3 and 6. The pH of the silages decreased during the fermentation as it was lower on days 3 and 6 than day 1 (P<0.05). On day 6 of the experiment, the number of lactic acid bacteria in MB15 was significantly (P<0.05) higher than in MB10. The percentage of non-protein nitrogen increased (P<0.05) on days 3 and 6 of fermentation. The amount of malondialdehyde indicated that the peroxidation ratio decreased (P<0.05) with increasing the fermentation time. Among the 23 lactic acid bacteria detected in MB15, nine isolates (39.1%) belonged to the species Lactobacillus plantarum, ten (43.5%) belonged to the species Lactobacillus rhamnosus, and four (43.5%) belonged to the species Lactobacillus fermentum. The isolates LPL9, LRH25, and LFE26 were identified as the strain of L. plantarum, L. ramnosus, and L. fermentum, respectively, that demonstrated the best characteristics for use as a probiotic. The results of this experiment indicated that the fermentation is an advisable method for reusing the nutrients of poultry slaughterhouse waste and producing a probiotic supplement.

Effect of extracted galactoglucomannan oligosaccharides from pine wood (Pinus brutia) on Salmonella typhimurium colonisation, growth performance and intestinal morphology in broiler chicks.

An in vitro and in vivo study was conducted to evaluate the fermentability of isolated galactoglucomannan oligosaccharides (GGMs) and the influence of their feeding on shedding and colonisation of Salmonella typhimurium, growth performance and intestinal morphology in broiler chicks. The in vitro data demonstrated that three probiotic lactic acid bacteria namely Lactobacillus casei, L. plantarum and Enterococcus faecium were able to ferment the extracted oligosaccharides and other tested sugars on a basal de Man Rogosa Sharpe media free from carbohydrate. For the in vivo experiment, 144 one-d-old male Ross 308 broiler chicks were divided into 6 experimental treatments (with 4 replicates) including two positive and negative controls which received a basal maize-soybean diet without any additives, supplementation of three levels of isolated GGMs (0.1%, 0.2% and 0.3%) and a commercial mannanoligosaccharide (MOS) at 0.2% to the basal diet. All birds except those in the negative control group were challenged orally with 1 × 108 cfu of S. typhimurium at 3-d post-hatch. The results revealed that challenge with S. typhimurium resulted in a significant reduction in body weight gain, feed intake, villus height, villus height to crypt depth ratio and villus surface area in all of infected chicks. Birds that were given GGMs or MOS showed better growth performance, increased villus height and villus surface area and decreased S. typhimurium colonisation than the positive control birds. GGM at 0.2% level was more effective than the other treatments in improving growth rate as well as gut health of broiler chicks.

Effect of calcium with and without probiotic, lactose, or both on organ and body weights, immune response and caecal microbiota in moulted laying hens.

A total of 72 laying hens were used to investigate the effect of probiotic and lactose on body weight loss, tibia ash, antibody production against sheep red blood cell (SRBC), heterophile-to-lymphocyte (H/L) ratio and gut microbiota in a common moulting method for 14 d. Hens were randomly allocated to 6 experimental groups consisting of (i) full feed (FF), (ii) feed withdrawal (FW), (iii) FW with calcium (Ca), (iv) FW with Ca and offering 7 g/lit lactose in drinking water (CaL), (v) FW with Ca and offering 1 g/lit probiotic in drinking water (CaP), and (vi) FW with Ca and offering a mixture of lactose and probiotic in drinking water (CaLP). The results showed body weight loss in all FW groups were more than 25% that was significantly higher than FF group (p < 0.05). The relative organ weights of hens in FW groups were lower than FF group; especially, it was significant for liver and ovary (p < 0.05). No significant difference was observed between all groups for tibia ash. The highest H/L ratio was related to FW group and offering Ca and lactose numerically and probiotic alone significantly resulted in decrease of this ratio (p < 0.05). The results also showed no significant difference for antibody production against SRBC among experimental groups. The highest coliform bacteria numeration observed in FW group and lactose could significantly reduce this population (p < 0.05). Lactic acid bacteria (LAB) numeration demonstrated a significant difference among treatments, so that FF hens had higher LAB than others (p < 0.05). In addition, FW moulted hens had significantly lower LAB compared to other moulted hens (p < 0.05), except for Ca group. In conclusion, probiotic and lactose was effective in maintaining caecal microbiota balance and improving immunity in hens exposed to moulting.

Effect of rosemary, echinacea, green tea extracts and ascorbic acid on broiler meat quality.

This study evaluated the effect of addition some plant extracts and ascorbic acid in presence of distilled water as the control on the broiler thigh meat color, subsequent lipid oxidation (TBARS) and rancidity development during frozen storage of chicken thigh meat. All the extracts were used in the density of 1000 ppm. The results showed that all the antioxidants had significant effect on lipid oxidation as measured by TBARS value during frozen storage at -20 degrees C for 120 days. However, lipid oxidation only occurred to a limited extent and was insufficient to cause rancid flavor development. The results also demonstrated that rosemary and green tea were the most effective antioxidants in stabilization of a* value. Echinacea, green tea and rosemary extracts were effective antioxidants and strongly inhibited oxidation. Present findings show that these plants extracts exhibit greater antioxidant efficiency compared to ascorbic acid.

Effects of different levels of hatchery wastes on the performance, carcass and tibia ash and some blood parameters in broiler chicks.

This experiment was conducted to evaluate the effects of different levels of Hatchery Wastes (HW) on performance, tibia ash, blood calcium and phosphorus concentrations in broiler chickens. Birds were fed a corn-soybean meal diet for 7 days. The experimental treatments included a corn soybean meal diet and 3 other treatments containing 1.5, 3.0 and 4.5% HW. five replicate groups of 15 Ross 308 broiler chicks were allocated to each dietary treatment. Data were analyzed in a completely randomized design. Results indicated that there were no significant differences in weight gains among different dietary treatments. Feed intake in the 4.5% treatment was significantly higher (p < 0.05) and 3% treatment had lower feed intake than the other treatments. The results indicated that feed conversion in the 3% HW treatment was lower in most of the period (p > 0.05). Results of carcass analysis showed no significant differences between treatments. Also, there were no significant differences between blood calcium and phosphorous among treatments. The 4.5 and 3.0% hatchery wastes treatments had the highest tibia ash (p < 0.05). Based on the result of this experiment, the utilization of hatchery wastes as much as 3% can increase tibia strength without having adverse effect on broilers performance.