Comparing different post-mortem human samples as DNA sources for downstream genotyping and identification.

The capability of DNA laboratories to perform genotyping procedures from post-mortem remains, including those that had undergone putrefaction, continues to be a challenge in the Philippines, a country characterized by very humid and warm conditions all year round. These environmental conditions accelerate the decomposition of human remains that were recovered after a disaster and those that were left abandoned after a crime. When considerable tissue decomposition of human remains has taken place, there is no other option but to extract DNA from bone and/or teeth samples. Routinely, femur shafts are obtained from recovered bodies for human identification because the calcium matrix protects the DNA contained in the osteocytes. In the Philippines, there is difficulty in collecting femur samples after natural disasters or even human-made disasters, because these events are usually characterized by a large number of fatalities. Identification of casualties is further delayed by limitation in human and material resources. Hence, it is imperative to test other types of biological samples that are easier to collect, transport, process and store. We analyzed DNA that were obtained from body fluid, bone marrow, muscle tissue, clavicle, femur, metatarsal, patella, rib and vertebral samples from five recently deceased untreated male cadavers and seven male human remains that were embalmed, buried for ∼ 1 month and then exhumed. The bodies had undergone different environmental conditions and were in various stages of putrefaction. A DNA extraction method utilizing a detergent-washing step followed by an organic procedure was used. The utility of bone marrow and vitreous fluid including bone marrow and vitreous fluid that was transferred on FTA(®) cards and subjected to autosomal STR and Y-STR DNA typing were also evaluated. DNA yield was measured and the presence or absence of PCR inhibitors in DNA extracts was assessed using Plexor(®)HY. All samples were amplified using PowerPlex(®)21 and PowerPlexY(®)23 systems and analyzed using the AB3500 Genetic Analyzer and the GeneMapper(®) ID-X v.1.2 software. PCR inhibitors were consistently detected in bone marrow, muscle tissue, rib and vertebra samples. Amplifiable DNA was obtained in a majority of the samples analyzed. DNA recovery from 0.1g biological material was adequate for successful genotyping of most of the non-bone and bone samples. Complete DNA profiles were generated from bone marrow, femur, metatarsal and patella with 0.1 ng DNA template. Using 0.5 ng DNA template resulted in increased allele recovery and improved intra- and inter-locus peak balance.

Complete mtDNA genomes of Filipino ethnolinguistic groups: a melting pot of recent and ancient lineages in the Asia-Pacific region.

The Philippines is a strategic point in the Asia-Pacific region for the study of human diversity, history and origins, as it is a cross-road for human migrations and consequently exhibits enormous ethnolinguistic diversity. Following on a previous in-depth study of Y-chromosome variation, here we provide new insights into the maternal genetic history of Filipino ethnolinguistic groups by surveying complete mitochondrial DNA (mtDNA) genomes from a total of 14 groups (11 groups in this study and 3 groups previously published) including previously published mtDNA hypervariable segment (HVS) data from Filipino regional center groups. Comparison of HVS data indicate genetic differences between ethnolinguistic and regional center groups. The complete mtDNA genomes of 14 ethnolinguistic groups reveal genetic aspects consistent with the Y-chromosome, namely: diversity and heterogeneity of groups, no support for a simple dichotomy between Negrito and non-Negrito groups, and different genetic affinities with Asia-Pacific groups that are both ancient and recent. Although some mtDNA haplogroups can be associated with the Austronesian expansion, there are others that associate with South Asia, Near Oceania and Australia that are consistent with a southern migration route for ethnolinguistic group ancestors into the Asia-Pacific, with a timeline that overlaps with the initial colonization of the Asia-Pacific region, the initial colonization of the Philippines and a possible separate post-colonization migration into the Philippine archipelago.

Field test of self-paced work capacity: Ifugao rice farmers.

This article reports results of a field test of work capacity on 30 male farmers ranging in age from 15-54. It involved a self-paced walk from the valley floor, up the mountain wall, and return, with heart rate monitoring. The route was 2.21 km long with a vertical rise of about 200 m. At its steepest, the grade was about 34 degrees, requiring long runs of steps cut into the mountain face. The purpose of this research was to determine whether biological and behavioral traits of individual men help to explain household economic productivity to which they contribute. The traits included anthropometry and a new measure of self-paced, voluntary work capacity (heart rate x time). Income per productive adult increased as did the relative fitness index (heart rate increase above resting x min taken to finish the course). However, the number of rice bundles earned per family, expected to increase with more fit workers, increased with the number of household adults and sitting height (adjusted R(2) = 0.392), but not with self-paced fitness markers. Am. J. Hum. Biol. 12:192-200, 2000. Copyright 2000 Wiley-Liss, Inc.

Physical size, school performance, and visual-motor maturity in the Philippines.

The broad theoretical issue approached in this work is whether supposedly adaptive or maladaptive biological variation has any real impact on people's lives and well-being. From a poor barrio of a rural lowland Philippine fishing community, a sample of 25 boys and 25 girls who had completed first grade at 8 years was measured. This population may be considered moderately undernourished by NCHS but not Philippine standards. Correlations between anthropometry, school grades, and visual-motor skills were calculated. Grades, taken here to have both cognitive and social-behavioral components, correlated best to percentage of median height (NCHS and Philippine), less strongly but still significantly to visual motor skill, and not to weight for height. Visual-motor skills (estimated by the Beery VMI) did not correlate to anthropometry. Visual-motor performance, however, is low by U.S. standards, a finding discussed in a behavioral context.