RESUMO
The influenza virus strain A/chicken/North Kazakhstan/184/2020 (H5N8) was isolated in North Kazakhstan during a highly pathogenic avian influenza outbreak in 2020. This study aimed to obtain the complete genome sequence of the isolate.
RESUMO
Nine monoclonal antibodies (MAB) against nucleocapsid protein (NP) of Newcastle disease virus (NDV) have been prepared and characterized. All the MABs were classified into three groups by means of the competitive binding assay. At least three antigenic sites were delineated on the NP. The 1st site includes two closely located epitopes; the 2nd site includes two related and two distinct epitopes; the 3rd site includes two closely related and one distinct epitopes.
Assuntos
Anticorpos Antivirais/imunologia , Antígenos Virais/imunologia , Vírus da Doença de Newcastle/imunologia , Nucleoproteínas/imunologia , Proteínas Virais/imunologia , Anticorpos Monoclonais , Antígenos Virais/classificação , Ligação Competitiva , Isotipos de Imunoglobulinas , Vírus da Doença de Newcastle/classificação , Proteínas do Nucleocapsídeo , Nucleoproteínas/classificação , Especificidade da Espécie , Proteínas Virais/classificaçãoRESUMO
From domestic birds 13 strains of avian paramyxoviruses, serotype 1, and 14 strains of serotype 2 were isolated. Avian paramyxoviruses, serotype 2, differ antigenically and biologically from each other and from the prototype variant chicken/Yucaipa/California/56. The virus was also detected experimentally in birds having contact with the infected specimens. Examinations of avian blood sera revealed wide dissemination of viruses related to the chicken/Yucaipa/56 strain in domestic bird breeding farms (43.6% to 50.0% of positive birds). The detected variability of the antigenic structure of the isolates attests to the potential emergence of a pathogenic variant.
Assuntos
Paramyxoviridae/isolamento & purificação , Animais , Anticorpos Antivirais/sangue , Antígenos Virais/sangue , Galinhas/microbiologia , Hemaglutininas Virais/sangue , Cazaquistão , Paramyxoviridae/imunologia , Paramyxoviridae/patogenicidade , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/microbiologia , Infecções por Respirovirus/imunologia , Infecções por Respirovirus/microbiologia , Infecções por Respirovirus/veterinária , Fatores de Tempo , Perus/microbiologiaRESUMO
Ninety-eight hemagglutinating agents were isolated from washings of cloaca and organs of 750 birds collected in southern and southeastern regions of the Kazakh SSR. Determinations of their type appurtenance allowed 36 agents to be classified into influenza A virus. Among them 4 strains had H1N1 surface antigens, 29 strains were Hav2 Nav5 and 3 strains had unidentified neuraminidase and Hav2. The data on the biological properties of influenza virus strains of both subtypes are presented.
