RESUMO
The preparation of the aminomethyl-bilinogen which results from formal "head to tail" condensation of porphobilinogen is described. The chemical cyclocondensation of this compound at pH 7.4 yields uroporphyrinogen I. Enzymatic studies with enzyme preparations from Propionibacterium shermanii, which synthesize uroporphyrinogens from porphobilinogen, show that the rate of cyclisation is increased by these enzymes and indicate that the bilinogen also might be used for uroporphyrinogen III formation. This is also suggested by studies on the formation of cobyrinic acid from [4-14C]5-aminolevulinate via uroporphyrinogen III in the presence of the aminomethylbilinogen by cell-free extracts from Clostridium tetanomorphum.
Assuntos
Porfobilinogênio/análogos & derivados , Uroporfirinogênios/biossíntese , Ácido Aminolevulínico/metabolismo , Clostridium/enzimologia , Hidroximetilbilano Sintase/metabolismo , Porfobilinogênio/síntese química , Porfobilinogênio/metabolismo , Propionibacterium/enzimologia , Uroporfirinogênio III Sintetase/metabolismo , Uroporfirinogênios/síntese química , Vitamina B 12/análogos & derivados , Vitamina B 12/biossínteseRESUMO
Cell-free extracts from Clostridium tetanomorphum, a microorganism which synthesizes corrins but no heme, are capable of converting both 5-aminolevulinate and uroporphyrinogen III into cobyrinic acid. Comparative examinations with (14C)5-aminolevulinate and (14C)uroporphyrinogen yielded corresponding results. Cell-free extracts from Clostridium tetanomorphum contain uroporphyrinogen III. To obtain good radiochemical yields it is therefore necessary to use substrates of high specific radioactivity. A method for the preparation of 14C-labelled uroporphyrin I-IV with high specific radioactivity is described.