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Biomed Microdevices ; 22(2): 37, 2020 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-32419044

RESUMO

In this work, it is presented a micro-optofluidic flow detector used for on-chip biological and chemical samples investigation. It is made in Poly-dimethyl-siloxane using a master-slave approach based on the 3D-Printing techniques. The micro-optofluidic device is made by assembling a microfluidic T-junction with a micro-optical section that consists of two optical fiber insertions and a PDMS gold-spattered micro-waveguide. The working principle in the detection is based on a different light transmission correlated to the fluid interfering with the laser beam in a micro-channel section. The proposed solution allows to realize a PDMS micro-device taking the advantage of 3D- Printing and goes beyond the restriction in the material selection. The device's performances were tested in the fluids detection and in the evaluation of the cell concentrations. Additionally, the micro-device was used as a real-time two-phase fluids flow detector. The two-phases flows were successfully monitored in different experimental conditions, varying both hydrodynamic and optical external stimuli.


Assuntos
Separação Celular/instrumentação , Dispositivos Lab-On-A-Chip , Dispositivos Ópticos , Impressão Tridimensional , Hidrodinâmica , Reprodutibilidade dos Testes
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