Impact of COVID-19 on Pregnancy Outcomes across Trimesters in the United States.

BACKGROUND: Current knowledge regarding the association between trimester-specific changes during pregnancy and COVID-19 infection is limited. We utilized the National Inpatient Sample (NIS) database to investigate trimester-specific outcomes among hospitalized pregnant women diagnosed with COVID-19. RESULTS: Out of 3,447,771 pregnant women identified, those with COVID-19 exhibited higher in-hospital mortality rates in their third trimester compared with those without the virus. Notably, rates of mechanical ventilation, acute kidney injury, renal replacement therapy, and perinatal complications (preeclampsia, HELLP syndrome, and preterm birth) were significantly elevated across all trimesters for COVID-19 patients. COVID-19 was found to be more prevalent among low-income, Hispanic pregnant women. CONCLUSIONS: Our findings suggest that COVID-19 during pregnancy is associated with increased risk of maternal mortality and complications, particularly in the third trimester. Furthermore, we observed significant racial and socioeconomic disparities in both COVID-19 prevalence and pregnancy outcomes. These findings emphasize the need for equitable healthcare strategies to improve care for diverse and socioeconomically marginalized groups, ultimately aiming to reduce adverse COVID-19-associated maternal and fetal outcomes.

Losartan-Induced Angioedema with Acute Hypoxemic Respiratory Failure (Anaphylaxis): A Case Report.

Angioedema is a condition characterized by swelling of the skin or mucosa resulting from loss of vascular integrity that leads to swelling of mucosal tissues and can lead to life-threatening respiratory compromise. Drug-induced angioedema is not a frequent side effect seen with angiotensin receptor blockers (ARBs), particularly when there are no other contributing risk factors like a prior episode. Few studies reported subsequent angioedema episodes after ARB use in patients who had a prior episode with angiotensin converting enzyme inhibitors; however, there are very few cases that documented non-fatal angioedema after ARB as the only therapy. We report a rare case of life-threatening anaphylaxis after losartan use. We hope that our case will bring awareness to this rare but fatal side effect in order to quickly recognize it and encourage further research.

Infrared spectroscopy enables rapid, robust, portable COVID-19 saliva screening based on pathophysiological response to SARS-CoV-2

Fourier-transform infrared (FTIR) spectroscopy provides a (bio)chemical snapshot of the sample, and was recently proposed for COVID-19 saliva screening in proof-of-concept cohort studies. As a step towards translation of this technology, we conducted controlled validation experiments in multiple biological systems. SARS-CoV-2 or UV-inactivated SARS-CoV-2 were used to infect Vero E6 cells in vitro, and K18-hACE2 mice in vivo. Potentially infectious culture supernatant or mouse oral lavage samples were treated with ethanol or Trizol to 75% (v/v) for attenuated total reflectance (ATR)-FTIR spectroscopy, or RT-PCR, respectively. The control condition, UV-inactivated SARS-CoV-2 elicited strong biochemical changes in culture supernatant/oral lavage despite lack of replication determined by RT-PCR or cell culture infectious dose 50%. Crucially, we show that active SARS-CoV-2 infection induced additional FTIR signals over the UV-inactivated SARS-CoV-2 infection, which correspond to innate immune response, aggregated proteins, and RNA. For human patient cohort prediction, we achieved high sensitivity of 93.48% on leave-on-out cross validation (n=104 participants) for predicting COVID-19 positivity using a partial least squares discriminant analysis model, in agreement with recent studies. However, COVID-19 patients negative on follow-up (RT-PCR on day of saliva sampling) were poorly predicted in this model. Importantly, COVID-19 vaccination did not lead to mis-classification of COVID-19 negatives. Meta-analysis revealed SARS-CoV-2 induced increase in Amide II band in all arms of this study and recent studies, indicative of altered {beta}-sheet structures in secreted proteins. In conclusion, ATR-FTIR is a robust, simple, portable method for COVID-19 saliva screening based on detection of pathophysiological responses to SARS-CoV-2.

Consumer governance and the provision of enabling services that facilitate access to care at community health centers.

BACKGROUND: Federally qualified health centers (FQHCs) are primary care clinics, governed by a consumer majority, which accept patients regardless of ability to pay and provide nonclinical enabling services that facilitate patients' access to care. Understanding how FQHCs decide which services to provide is important, because enabling services are not typically reimbursed. OBJECTIVE: To model enabling service provision as a function of FQHC board composition. METHODS: FQHC-level data were drawn from multiple years of the Uniform Data System (UDS) (2002-2007), and merged with county-level data from the Area Resource File (ARF) (2002-2007) and board data from FQHC grant applications (2003-2006). The scope and volume of enabling services an FQHC provides are modeled as a function of board composition, executive committee composition, the interaction between them, general time trends, and other FQHC and county-level controls. RESULTS: The proportion of consumers on the board does not affect the scope of enabling services, but the proportion of descriptive consumers (who resemble typical FQHC patients) on the executive committee is associated with a significant increase in the scope of enabling services a health center provides. Neither the proportion of consumers on the board nor the proportion of consumers on the executive committee affected the volume of enabling services provided. CONCLUSIONS: Consumer governance, specifically on the executive committee, plays a small role in determining which enabling services an FQHC provides, but more work is needed to identify factors associated with variation in the scope and volume of enabling services across FQHCs.

Salvaging kidneys with renal allograft compartment syndrome.

Renal allograft compartment syndrome is an under recognized cause of early allograft dysfunction which can be reversed by early intervention. It occurs early after renal transplantation where closure of the anterior abdominal wall seems to compress the transplant in the limited retroperitoneal space. The literature about this syndrome in renal transplantation is sparse. Our report describes the diagnostic criteria and the management of two renal transplant recipients with this syndrome. Its diagnosis depends upon duplex vascular scan findings of reversed or absent diastolic flow in the renal vasculature in the absence of any perigraft collection or severe acute tubular necrosis. In our hands emergency laparotomy, decompression of the transplant and closure with interposition mesh salvaged these kidneys.

Mechanical properties of the avian acrocoracohumeral ligament and its role in shoulder stabilization in flight.

Control of movement in the avian shoulder joint is fundamental to understanding the avian wingstroke. The acrocoracohumeral ligament (AHL) is thought to play a key role in stabilizing the glenoid and balancing the pectoralis in gliding flight. If the AHL has to be taut to balance the pectoralis, then it must constrain glenohumeral motion during flapping flight as well. However, birds vary wing kinematics depending on flight speed and behavior. How can a passive ligament accommodate such varying joint movements? Herein, mechanical testing and 3-D modeling are used to link the mechanical properties and morphology of the AHL to its functional role during flapping flight. The bone-ligament-bone complex of the pigeon (Columba livia) fails at a tensile loading of 141 ± 18 N (± s .D., n = 10) or 39 times body weight, which corresponds to a failure stress of 51 MPa, well above expected loads during flight. Simulated AHL length changes, comparisons to glenohumeral kinematics from the literature, and manipulations of partially dissected pigeon specimens all support the hypothesis that the AHL remains taut through downstroke and most of upstroke while becoming slack during the downstroke/upstroke transition. The digital AHL model provides a mechanism for explaining how the AHL can stabilize the shoulder joint under a broad array of humeral paths by constraining the coordination of glenohumeral degrees of freedom.

Time is money: opportunity cost and physicians' provision of charity care 1996-2005.

OBJECTIVE: To test whether physicians' provision of charity care depends on their hourly wage. DATA SOURCES: Secondary data from four rounds of the Community Tracking Study (CTS) Physician Survey (1996-2005). Data are nationally representative of nonfederal office- and hospital-based physicians spending at least 20 hours per week on patient care. STUDY DESIGN: A two-part model with site-level fixed effects, time trend variables, and site-year interactions is used to model the relationship between physicians' hourly wage and both their decision to provide any charity care and the amount of charity care provided. Salaried and nonsalaried physicians are modeled separately. DATA COLLECTION/EXTRACTION METHODS: Data from each round of the CTS were merged into a single cross-sectional file with 38,087 physician-year observations. PRINCIPAL FINDINGS: The association between physician's hourly wage and the likelihood of providing charity care is positive for salaried physicians and negative for nonsalaried physicians. Among physicians providing any charity care, hourly wage is positively associated with the amount of charity care provided regardless of salaried status. Practice characteristics are also significant. CONCLUSIONS: The financial considerations of salaried physicians differ significantly from those of nonsalaried physicians in the decision to provide charity care, but factor similarly into the amount of charity care provided.

Pancreas allograft rejection: analysis of concurrent renal allograft biopsies and posttherapy follow-up biopsies.

BACKGROUND: Pancreas and kidney allograft function is routinely monitored with serum studies (amylase, lipase, and creatinine). Increased levels commonly prompt tissue biopsy, to diagnose cause of graft dysfunction. Historically, pancreas allografts were infrequently biopsied, although serum enzymes and renal rejection may be poor surrogates for pancreas status. METHODS: Pancreas allograft biopsies at our center were reviewed and reclassified according to University of Maryland (UMD) and Banff criteria; C4d immunostaining was performed. Findings were correlated with clinical data and renal allograft biopsies. RESULTS: Fifty-six pancreas allograft biopsies from 27 patients were evaluated. UMD and Banff grading were similar, although two UMD "indeterminate" biopsies were Banff grade 1 rejection. There were 21 concurrent pancreas and renal biopsies, all from simultaneous pancreas-kidney allograft recipients. Thirteen pairs were concordant for rejection; eight pairs were discordant for rejection (38%); six pairs showed pancreas rejection without kidney rejection, and two pairs showed the converse. Fourteen patients had a total of 21 follow-up pancreas allograft biopsies. Seven biopsies showed a lower grade of rejection on follow-up biopsy, 4 biopsies showed more severe rejection, and 10 had unchanged grade. In only 9 of these 21 (43%) cases, did the interval serum amylase or lipase trend parallel the subsequent biopsy diagnosis. CONCLUSIONS: With a high biopsy discordance rate, our data suggest that renal allograft rejection is a poor surrogate for pancreas allograft status. Likewise, serum amylase and lipase levels do not predict response to rejection therapy. Surveillance or posttherapy pancreas allograft biopsies may be a useful means to monitor pancreas allograft status.

The road to efficiency? Re-examining the impact of the primary care physician workforce on health care utilization rates.

Research suggests that primary care physicians may help to control health care costs by encouraging more efficient service use. However, most studies do not account for data aggregation effects that can significantly affect the direction and magnitude of findings. To re-examine the association between the proportion of primary care physicians and health care utilization rates in an area, and investigate the potential impact of aggregating data to different geographic levels on these observed associations, we estimate four distinct cross-sectional multivariate regression models to predict health care utilization at the county level and the metropolitan statistical area (MSA) level using data from 2007. Our study focuses on health care utilization in the United States using inpatient admissions, outpatient visits, emergency room visits, and total (both inpatient and outpatient) surgeries as dependent variables in separate regressions. The key independent variable is the proportion of primary care physicians in the area. Several community-level control variables are also included. We find that a higher proportion of primary care physicians in the area's physician supply is associated with a decreased number of inpatient admissions at the MSA level, but not the county level, and a decreased number of emergency room visits at the county level, but not the MSA level. Outpatient visits and total surgeries are not associated with the proportion of primary care physicians. From our findings we are able to conclude that there is some evidence that a higher concentration of primary care physicians is associated with a decrease in health care utilization, but these findings depend on the level of aggregation. Investigators should be aware of the implications of aggregating data and acknowledge any resultant limitations.

Review of X-ray Phase Contrast Imaging Techniques and Propagation Based Imaging Using a Benchtop Microfocal Source / Jurnal Sains Kesihatan Malaysia

Currently, the basis for standard clinical X-ray radiography is absorption, tracking attenuation of radiation when X-ray beams pass through a particular part of the body. The fundamental interaction processes are dependent on variations in the atomic number and density of the target medium; consequentially radiographic contrast can be poor, particularly in regard to soft tissue imaging. Over the past several years considerable interest has been paid to utilising phase properties of the X-ray field to enhance radiographic contrast in challenging circumstances, particularly with respect to soft-tissue imaging. Surrey is among an increasing number of institutes in which a programme of investigation of phase contrast X-ray imaging has been established. We briefly review some basic ideas in X-ray phase contrast imaging and then examine the radiographic contrast enhancement that can be obtained, use being made of the method of free-space propagation and investigating a number of test objects, including biological samples. The equipment used at Surrey is relatively simple, comprising of bench-top X-ray tubes with focal spot sizes from a few microns up to 100 microns and a position sensitive 12 bit CCD detector.