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1.
Infect Immun ; 87(6)2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30962402

RESUMO

Giardia lamblia, one of the most common protozoal infections of the human intestine, is an important worldwide cause of diarrheal disease, malabsorption, malnutrition, delayed cognitive development in children, and protracted postinfectious syndromes. Despite its medical importance, no human vaccine is available against giardiasis. A crude veterinary vaccine has been developed, and experimental vaccines based on expression of multiple variant-specific surface proteins have been reported, but poorly defined vaccine components and excessive antigen variability are problematic for pharmaceutical vaccine production. To expand the repertoire of antigen candidates for vaccines, we reasoned that surface proteins may provide an enriched source of such antigens since key host effectors, such as secretory IgA, can directly bind to such antigens in the intestinal lumen and interfere with epithelial attachment. Here, we have applied a proteomics approach to identify 23 novel surface antigens of G. lamblia that show >90% amino acid sequence identity between the two human-pathogenic genetic assemblages (A and B) of the parasite. Surface localization of a representative subset of these proteins was confirmed by immunostaining. Four selected proteins, uridine phosphorylase-like protein-1, protein 21.1 (GL50803_27925), α1-giardin, and α11-giardin, were subsequently produced in recombinant form and shown to be immunogenic in mice and G. lamblia-infected humans and confer protection against G. lamblia infection upon intranasal immunization in rodent models of giardiasis. These results demonstrate that identification of conserved surface antigens provides a powerful approach for overcoming a key rate-limiting step in the design and construction of an effective vaccine against giardiasis.


Assuntos
Antígenos de Protozoários/imunologia , Giardia lamblia/imunologia , Giardíase/parasitologia , Proteoma/imunologia , Proteínas de Protozoários/imunologia , Vacinas Protozoárias/imunologia , Adulto , Animais , Anticorpos Antiprotozoários/imunologia , Antígenos de Protozoários/química , Antígenos de Protozoários/genética , Reações Cruzadas , Feminino , Giardia lamblia/química , Giardia lamblia/genética , Giardíase/imunologia , Giardíase/prevenção & controle , Humanos , Masculino , Espectrometria de Massas , Camundongos , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , Proteoma/química , Proteoma/genética , Proteínas de Protozoários/química , Proteínas de Protozoários/genética , Vacinas Protozoárias/química , Vacinas Protozoárias/genética , Adulto Jovem
2.
Mol Biochem Parasitol ; 197(1-2): 21-3, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25286381

RESUMO

Serial Analysis of Gene Expression (SAGE) was used to quantify transcriptional changes in Giardia intestinalis during its interaction with human intestinal epithelial cells (IECs, HT-29) in serum free M199 medium. Transcriptional changes were compared to those in trophozoites alone in M199 and in TYI-S-33 Giardia growth medium. In total, 90 genes were differentially expressed, mainly those involved in cellular redox homeostasis, metabolism and small molecule transport but also cysteine proteases and structural proteins of the giardin family. Only 29 genes changed their expression due to IEC interaction and the rest were due to M199 medium. Although our findings generated a small dataset, it was consistent with our earlier microarray studies performed under different interaction conditions. This study has confined the number of genes in Giardia to a small subset that specifically change their expression due to interaction with IECs.


Assuntos
Células Epiteliais/metabolismo , Células Epiteliais/parasitologia , Expressão Gênica , Giardia/fisiologia , Interações Hospedeiro-Patógeno/genética , Linhagem Celular , Meios de Cultura Livres de Soro , Perfilação da Expressão Gênica , Giardíase/genética , Giardíase/parasitologia , Humanos , Mucosa Intestinal , Transcriptoma
3.
Mol Biochem Parasitol ; 184(2): 122-5, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22569588

RESUMO

The ability of Giardia to differentiate into cysts which survive in the environment and release the virulent trophozoites after ingestion in the small intestine is essential for transmission and disease. We examined the role of enolase, a glycolytic enzyme, in Giardia differentiation. The sequence of Giardia lamblia enolase (gEno) is most similar to enolases in Homo sapiens and Leishmania mexicana, and shows the conserved catalytic and metal-binding residues. We used an integration vector to stably express wild type and mutant gEno. In trophozoites, wild type gEno localized to the cell membrane, caudal flagella and cytosol. gEno is present on the wall of mature cysts, but not in encystation secretory vesicles (ESV). The expression of gEno with a deletion of residues G167-K169, or mutations H389Q/R390S significantly inhibited excystation while mutation of residue D257K had no effect. These results suggest a role for enolase in regulation of Giardia excystation.


Assuntos
Giardia lamblia/enzimologia , Fosfopiruvato Hidratase/metabolismo , Proteínas de Protozoários/metabolismo , Sequência de Aminoácidos , Domínio Catalítico , Sequência Consenso , Giardia lamblia/citologia , Giardia lamblia/fisiologia , Dados de Sequência Molecular , Mutagênese , Fosfopiruvato Hidratase/genética , Proteínas de Protozoários/genética , Deleção de Sequência , Trofozoítos/enzimologia
4.
Int J Parasitol ; 42(4): 411-9, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22429767

RESUMO

The NIMA-related serine/threonine kinases (Neks) function in the cell cycle and regulate ciliary and flagellar length. The Giardia lamblia genome encodes 198 Neks, of which 56 are predicted to be active. Here we believe that we report the first functional analysis of two G. lamblia Neks. The GlNek1 and GlNek2 kinase domains share 57% and 43% identity to the kinase domains of human Nek1 and Nek2, respectively. Both GlNeks are active in vitro, have dynamic relocalisation during the cell cycle, and are expressed throughout the life cycle, with GlNek1 being upregulated in cysts. Over-expression of inactive GlNek1 delays disassembly of the parental attachment disc and cytokinesis, whilst over-expression of either wild type GlNek1 or inactive mutant GlNek2 inhibits excystation.


Assuntos
Giardia lamblia/enzimologia , Giardia lamblia/fisiologia , Mitose , Proteínas Serina-Treonina Quinases/metabolismo , Sequência de Aminoácidos , Citosol/química , Perfilação da Expressão Gênica , Giardia lamblia/crescimento & desenvolvimento , Dados de Sequência Molecular , Proteínas de Protozoários/metabolismo , Homologia de Sequência de Aminoácidos
5.
Vaccine ; 29(51): 9529-37, 2011 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-22001876

RESUMO

Giardia lamblia is a leading protozoan cause of diarrheal disease worldwide, yet preventive medical strategies are not available. A crude veterinary vaccine has been licensed for cats and dogs, but no defined human vaccine is available. We tested the vaccine potential of three conserved antigens previously identified in human and murine giardiasis, α1-giardin, α-enolase, and ornithine carbamoyl transferase, in a murine model of G. lamblia infection. Live recombinant attenuated Salmonella enterica Serovar Typhimurium vaccine strains were constructed that stably expressed each antigen, maintained colonization capacity, and sustained total attenuation in the host. Oral administration of the vaccine strains induced antigen-specific serum IgG, particularly IgG(2A), and mucosal IgA for α1-giardin and α-enolase, but not for ornithine carbamoyl transferase. Immunization with the α1-giardin vaccine induced significant protection against subsequent G. lamblia challenge, which was further enhanced by boosting with cholera toxin or sublingual α1-giardin administration. The α-enolase vaccine afforded no protection. Analysis of α1-giardin from divergent assemblage A and B isolates of G. lamblia revealed >97% amino acid sequence conservation and immunological cross-reactivity, further supporting the potential utility of this antigen in vaccine development. Together. These results indicate that α1-giardin is a suitable candidate antigen for a vaccine against giardiasis.


Assuntos
Proteínas do Citoesqueleto/imunologia , Giardia lamblia/imunologia , Giardíase/prevenção & controle , Proteínas de Protozoários/imunologia , Vacinas Protozoárias/imunologia , Administração Oral , Animais , Anticorpos Antiprotozoários/biossíntese , Antígenos de Protozoários/imunologia , Toxina da Cólera/imunologia , Proteínas do Citoesqueleto/administração & dosagem , Giardíase/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Ornitina Carbamoiltransferase/administração & dosagem , Ornitina Carbamoiltransferase/imunologia , Fosfopiruvato Hidratase/administração & dosagem , Fosfopiruvato Hidratase/imunologia , Proteínas de Protozoários/administração & dosagem , Vacinas Protozoárias/administração & dosagem , Salmonella typhimurium/genética , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/imunologia
6.
Antimicrob Agents Chemother ; 55(10): 4643-51, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21825286

RESUMO

Infections with the diarrheagenic protozoan pathogen Giardia lamblia are most commonly treated with metronidazole (Mz). Treatment failures with Mz occur in 10 to 20% of cases and Mz resistance develops in the laboratory, yet clinically, Mz-resistant (Mz(r)) G. lamblia has rarely been isolated from patients. To understand why clinical Mz(r) isolates are rare, we questioned whether Mz resistance entails fitness costs to the parasite. Our studies employed several newly generated and established isogenic Mz(r) cell lines with stable, high-level resistance to Mz and significant cross-resistance to tinidazole, nitazoxanide, and furazolidone. Oral infection of suckling mice revealed that three of five Mz(r) cell lines could not establish infection, while two Mz(r) cell lines infected pups, albeit with reduced efficiencies. Failure to colonize resulted from a diminished capacity of the parasite to attach to the intestinal mucosa in vivo and to epithelial cells and plastic surfaces in vitro. The attachment defect was related to impaired glucose metabolism, since the noninfectious Mz(r) lines consumed less glucose, and glucose promoted ATP-independent parasite attachment in the parental lines. Thus, resistance of Giardia to Mz is accompanied by a glucose metabolism-related attachment defect that can interfere with colonization of the host. Because glucose-metabolizing pathways are important for activation of the prodrug Mz, it follows that a fitness trade-off exists between diminished Mz activation and reduced infectivity, which may explain the observed paucity of clinical Mz(r) isolates of Giardia. However, the data also caution that some forms of Mz resistance do not markedly interfere with in vivo infectivity.


Assuntos
Antiprotozoários/farmacologia , Resistência a Medicamentos , Giardia lamblia/efeitos dos fármacos , Giardíase/parasitologia , Metronidazol/farmacologia , Animais , Linhagem Celular , Furazolidona/farmacologia , Giardia lamblia/metabolismo , Giardia lamblia/fisiologia , Giardíase/tratamento farmacológico , Glucose/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Nitrocompostos , Tiazóis/farmacologia , Tinidazol/farmacologia
7.
Int J Parasitol ; 41(10): 1079-92, 2011 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-21723868

RESUMO

Giardia lamblia is a flagellated protozoan parasite and a major cause of diarrhoea in humans. Its microtubular cytoskeleton mediates trophozoite motility, attachment and cytokinesis, and is characterised by an attachment disk and eight flagella that are each nucleated in a basal body. To date, only 10 giardial basal body proteins have been identified, including universal signalling proteins that are important for regulating mitosis or differentiation. In this study, we have exploited bioinformatics and proteomic approaches to identify new Giardia basal body proteins and confocal microscopy to confirm their localisation in interphase trophozoites. This approach identified 75 homologs of conserved basal body proteins in the genome including 65 not previously known to be associated with Giardia basal bodies. Thirteen proteins were confirmed to co-localise with centrin to the Giardia basal bodies. We also demonstrate that most basal body proteins localise to additional cytoskeletal structures in interphase trophozoites. This might help to explain the roles of the four pairs of flagella and Giardia-specific organelles in motility and differentiation. A deeper understanding of the composition of the Giardia basal bodies will contribute insights into the complex signalling pathways that regulate its unique cytoskeleton and the biological divergence of these conserved organelles.


Assuntos
Genoma de Protozoário , Giardia lamblia/química , Giardia lamblia/genética , Organelas/química , Organelas/genética , Proteoma/análise , Proteínas de Protozoários/análise , Biologia Computacional , Genes de Protozoários , Microscopia Confocal
8.
Mol Biochem Parasitol ; 174(1): 62-5, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20570699

RESUMO

We quantified mRNA abundance from 10 stages in the Giardia lamblia life cycle in vitro using Serial Analysis of Gene Expression (SAGE). 163 abundant transcripts were expressed constitutively. 71 transcripts were upregulated specifically during excystation and 42 during encystation. Nonetheless, the transcriptomes of cysts and trophozoites showed major differences. SAGE detected co-expressed clusters of 284 transcripts differentially expressed in cysts and excyzoites and 287 transcripts in vegetative trophozoites and encysting cells. All clusters included known genes and pathways as well as proteins unique to Giardia or diplomonads. SAGE analysis of the Giardia life cycle identified a number of kinases, phosphatases, and DNA replication proteins involved in excystation and encystation, which could be important for examining the roles of cell signaling in giardial differentiation. Overall, these data pave the way for directed gene discovery and a better understanding of the biology of G. lamblia.


Assuntos
Perfilação da Expressão Gênica , Giardia lamblia/crescimento & desenvolvimento , Giardia lamblia/genética , Estágios do Ciclo de Vida , Animais , Proteínas de Protozoários/biossíntese , Proteínas de Protozoários/fisiologia
9.
J Med Chem ; 52(13): 4038-53, 2009 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-19480409

RESUMO

Infections with the diarrheagenic pathogen, Giardia lamblia, are commonly treated with the 5-nitroimidazole (5-NI) metronidazole (Mz), and yet treatment failures and Mz resistance occur. Using a panel of new 2-ethenyl and 2-ethanyl 5-NI derivatives, we found that compounds with a saturated bridge between the 5-NI core and a pendant ring system exhibited only modestly increased antigiardial activity and could not overcome Mz resistance. By contrast, olefins with a conjugated bridge connecting the core and a substituted phenyl or heterocyclic ring showed greatly increased antigiardial activity without toxicity, and several overcame Mz resistance and were more effective than Mz in a murine giardiasis model. Determination of the half-wave potential of the initial one-electron transfer by cyclic voltammetry revealed that easier redox activation correlated with greater antigiardial activity and capacity to overcome Mz resistance. These studies show the potential of combining systematic synthetic approaches with biological and electrochemical evaluations in developing improved 5-NI drugs.


Assuntos
Antiprotozoários/química , Técnicas Eletroquímicas/métodos , Giardia lamblia/efeitos dos fármacos , Nitroimidazóis/química , Animais , Antiprotozoários/farmacologia , Descoberta de Drogas , Resistência a Medicamentos , Giardíase/tratamento farmacológico , Metronidazol/farmacologia , Nitroimidazóis/farmacologia , Oxirredução
10.
Mol Biochem Parasitol ; 159(2): 85-91, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18359106

RESUMO

Giardia lamblia, an important cause of diarrheal disease, resides in the small intestinal lumen in close apposition to epithelial cells. Since the disease mechanisms underlying giardiasis are poorly understood, elucidating the specific interactions of the parasite with the host epithelium is likely to provide clues to understanding the pathogenesis. Here we tested the hypothesis that contact of Giardia lamblia with intestinal epithelial cells might lead to release of specific proteins. Using established co-culture models, intestinal ligated loops and a proteomics approach, we identified three G. lamblia proteins (arginine deiminase, ornithine carbamoyl transferase and enolase), previously recognized as immunodominant antigens during acute giardiasis. Release was stimulated by cell-cell interactions, since only small amounts of arginine deiminase and enolase were detected in the medium after culturing of G. lamblia alone. The secreted G. lamblia proteins were localized to the cytoplasm and the inside of the plasma membrane of trophozoites. Furthermore, in vitro studies with recombinant arginine deiminase showed that the secreted Giardia proteins can disable host innate immune factors such as nitric oxide production. These results indicate that contact of Giardia with epithelial cells triggers metabolic enzyme release, which might facilitate effective colonization of the human small intestine.


Assuntos
Células Epiteliais/parasitologia , Giardia lamblia/enzimologia , Proteínas de Protozoários/isolamento & purificação , Proteínas de Protozoários/metabolismo , Animais , Linhagem Celular , Membrana Celular/enzimologia , Técnicas de Cocultura , Citoplasma/enzimologia , Eletroforese em Gel Bidimensional , Giardia lamblia/imunologia , Humanos , Hidrolases/isolamento & purificação , Hidrolases/metabolismo , Óxido Nítrico/antagonistas & inibidores , Ornitina Carbamoiltransferase/isolamento & purificação , Ornitina Carbamoiltransferase/metabolismo , Fosfopiruvato Hidratase/isolamento & purificação , Fosfopiruvato Hidratase/metabolismo , Proteômica , Trofozoítos/enzimologia
11.
Int J Parasitol ; 38(3-4): 353-69, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17964578

RESUMO

Giardia lamblia is a major cause of diarrhoeal disease worldwide. Since it has no known toxin, the ability of trophozoites to colonise the human small intestine is required for its pathogenesis. Mitosis in this protozoan parasite is a unique challenge because its two equivalent nuclei and complex cytoskeleton must be duplicated and segregated accurately. Giardial mitosis is a complex and rapid event that is poorly understood at the cellular and molecular levels. Higher eukaryotes have one to three members of the highly conserved Ser/Thr aurora kinase (AK) family that regulate key aspects of mitosis and cytokinesis. Giardia has a single AK orthologue (gAK) with 61% similarity to human AK A. In addition to the conserved active site residues, activation loop and destruction-box motifs characteristic of AKs, gAK contains a unique insert near the active site region. We epitope-tagged gAK at its C-terminus and expressed it under its own promoter. During interphase, gAK localises exclusively to the nuclei, but is not phosphorylated as shown by lack of staining with an antibody specific to phosphorylated AK A (pAK). In contrast, during mitosis pAK localises to the basal bodies/centrosomes and co-localises with tubulin to the spindle. During specific stages of mitosis, giardial pAK also localised dynamically to cytoskeletal structures unique to Giardia: the paraflagellar dense rods of the anterior flagella and the median body, whose functions are unknown, as well as to the parent attachment disc. Two AK inhibitors significantly decreased giardial growth and increased the numbers of cells arrested in cytokinesis. These inhibitors appeared to increase microtubule nucleation and cell-ploidy. Our data show that gAK is phosphorylated in mitosis and suggest that it plays an important role in the Giardia cell cycle. The pleiotropic localisation of AK suggests that it may co-ordinate the reorganisation and segregation of tubulin-containing structures in mitosis. We believe this is the first report of a signalling protein regulating cell division in Giardia.


Assuntos
Antígenos de Protozoários/genética , Giardia lamblia/enzimologia , Mitose/fisiologia , Proteínas Serina-Treonina Quinases/genética , Animais , Antígenos de Protozoários/análise , Aurora Quinase A , Aurora Quinases , Sequência de Bases , Centrossomo/enzimologia , Diarreia/parasitologia , Inibidores Enzimáticos/farmacologia , Expressão Gênica , Interações Hospedeiro-Parasita , Humanos , Enteropatias Parasitárias/imunologia , Microtúbulos/enzimologia , Dados de Sequência Molecular , Parasitologia/métodos , Proteínas Serina-Treonina Quinases/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência
12.
Curr Opin Microbiol ; 10(6): 554-9, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17981075

RESUMO

To colonize the human small intestine, Giardia lamblia monitors a dynamic environment. Trophozoites attach to enterocytes that mature and die. The parasites must 'decide' whether to re-attach or differentiate into cysts that survive in the environment and re-activate when ingested. Other intestinal parasites face similar challenges. Study of these parasites is limited because they do not encyst in vitro. Giardia trophozoites were persuaded to encyst in vitro by mimicking physiological stimuli. Cysts are dormant, yet 'spring-loaded for action' to excyst upon ingestion. Giardial encystation has been studied from morphological, cell biological, biochemical, and molecular viewpoints. Yet important gaps remain and the mechanisms that co-ordinate responses to external signals remain enigmatic.


Assuntos
Giardia lamblia/fisiologia , Animais , Parede Celular/metabolismo , Regulação da Expressão Gênica , Giardia lamblia/citologia , Giardia lamblia/genética , Giardia lamblia/metabolismo , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismo
13.
Science ; 317(5846): 1921-6, 2007 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-17901334

RESUMO

The genome of the eukaryotic protist Giardia lamblia, an important human intestinal parasite, is compact in structure and content, contains few introns or mitochondrial relics, and has simplified machinery for DNA replication, transcription, RNA processing, and most metabolic pathways. Protein kinases comprise the single largest protein class and reflect Giardia's requirement for a complex signal transduction network for coordinating differentiation. Lateral gene transfer from bacterial and archaeal donors has shaped Giardia's genome, and previously unknown gene families, for example, cysteine-rich structural proteins, have been discovered. Unexpectedly, the genome shows little evidence of heterozygosity, supporting recent speculations that this organism is sexual. This genome sequence will not only be valuable for investigating the evolution of eukaryotes, but will also be applied to the search for new therapeutics for this parasite.


Assuntos
Evolução Biológica , Células Eucarióticas , Genoma de Protozoário , Giardia lamblia/genética , Sequência de Aminoácidos , Animais , Replicação do DNA/genética , Transferência Genética Horizontal , Genes de Protozoários , Genômica , Giardia lamblia/classificação , Giardia lamblia/fisiologia , Redes e Vias Metabólicas/genética , Dados de Sequência Molecular , Filogenia , Proteínas Quinases/genética , Proteínas Quinases/metabolismo , Proteínas de Protozoários/química , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismo , Processamento Pós-Transcricional do RNA , Transdução de Sinais , Transcrição Gênica
14.
Mol Biochem Parasitol ; 152(1): 80-9, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17204341

RESUMO

The ability of Giardia lamblia to undergo two distinct differentiations in response to physiologic stimuli is central to its pathogenesis. The giardial cytoskeleton changes drastically during encystation and excystation. However, the signal transduction pathways mediating these transformations are poorly understood. We tested the hypothesis that PP2A, a highly conserved serine/threonine protein phosphatase, might be important in giardial differentiation. We found that in vegetatively growing trophozoites, gPP2A-C protein localizes to basal bodies/centrosomes, and to cytoskeletal structures unique to Giardia: the ventral disk, and the dense rods of the anterior, posterior-lateral, and caudal flagella. During encystation, gPP2A-C protein disappears from only the anterior flagellar dense rods. During excystation, gPP2A-C localizes to the cyst wall in excysting cysts but is not found in the wall of cysts with emerging excyzoites. Transcriptome and immunoblot analyses indicated that gPP2A-C mRNA and protein are upregulated in mature cysts and during the early stage of excystation that models passage through the host stomach. Stable expression of gPP2A-C antisense RNA did not affect vegetative growth, but strongly inhibited the formation of encystation secretory vesicles (ESV) and water-resistant cysts. Moreover, the few cysts that formed were highly defective in excystation. Thus, gPP2A-C localizes to universal cytoskeletal structures and to structures unique to Giardia. It is also important for encystation and excystation, crucial giardial transformations that entail entry into and exit from dormancy.


Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Giardia lamblia/enzimologia , Giardia lamblia/crescimento & desenvolvimento , Fosfoproteínas Fosfatases/metabolismo , Adaptação Fisiológica , Sequência de Aminoácidos , Animais , Western Blotting , Centrossomo/química , Citoesqueleto/química , DNA de Protozoário/química , DNA de Protozoário/genética , Flagelos/química , Giardia lamblia/genética , Imuno-Histoquímica , Microscopia de Fluorescência , Dados de Sequência Molecular , Morfogênese/genética , Fosfoproteínas Fosfatases/biossíntese , Fosfoproteínas Fosfatases/genética , Proteína Fosfatase 2 , Proteínas de Protozoários/análise , Proteínas de Protozoários/genética , RNA de Protozoário/análise , RNA de Protozoário/genética , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos
15.
PLoS One ; 1: e44, 2006 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-17183673

RESUMO

Since the Giardia lamblia cyst wall is necessary for survival in the environment and host infection, we tested the hypothesis that it contains proteins other than the three known cyst wall proteins. Serial analysis of gene expression during growth and encystation revealed a gene, "HCNCp" (High Cysteine Non-variant Cyst protein), that was upregulated late in encystation, and that resembled the classic Giardia variable surface proteins (VSPs) that cover the trophozoite plasmalemma. HCNCp is 13.9% cysteine, with many "CxxC" tetrapeptide motifs and a transmembrane sequence near the C-terminus. However, HCNCp has multiple "CxC" motifs rarely found in VSPs, and does not localize to the trophozoite plasmalemma. Moreover, the HCNCp C-terminus differed from the canonical VSP signature. Full-length epitope-tagged HCNCp expressed under its own promoter was upregulated during encystation with highest expression in cysts, including 42 and 21 kDa C-terminal fragments. Tagged HCNCp targeted to the nuclear envelope in trophozoites, and co-localized with cyst proteins to encystation-specific secretory vesicles during encystation. HCNCp defined a novel trafficking pathway as it localized to the wall and body of cysts, while the cyst proteins were exclusively in the wall. Unlike VSPs, HCNCp is expressed in at least five giardial strains and four WB subclones expressing different VSPs. Bioinformatics identified 60 additional large high cysteine membrane proteins (HCMp) containing > or = 20 CxxC/CxC's lacking the VSP-specific C-terminal CRGKA. HCMp were absent or rare in other model or parasite genomes, except for Tetrahymena thermophila with 30. MEME analysis classified the 61 gHCMp genes into nine groups with similar internal motifs. Our data suggest that HCNCp is a novel invariant cyst protein belonging to a new HCMp family that is abundant in the Giardia genome. HCNCp and the other HCMp provide a rich source for developing parasite-specific diagnostic reagents, vaccine candidates, and subjects for further research into Giardia biology.


Assuntos
Genes de Protozoários , Giardia lamblia/genética , Proteínas de Protozoários/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Biologia Computacional , Cisteína/química , Primers do DNA/genética , Variação Genética , Giardia lamblia/química , Giardia lamblia/patogenicidade , Giardíase/parasitologia , Interações Hospedeiro-Patógeno/genética , Humanos , Dados de Sequência Molecular , Proteínas de Protozoários/química , RNA Mensageiro/genética , RNA de Protozoário/genética , Regulação para Cima
16.
J Immunol ; 177(9): 6281-90, 2006 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-17056558

RESUMO

The polymeric Ig receptor (pIgR) is conserved in mammals and has an avian homologue, suggesting evolutionarily important functions in vertebrates. It transports multimeric IgA and IgM across polarized epithelia and is highly expressed in the intestine, yet little direct evidence exists for its importance in defense against common enteric pathogens. In this study, we demonstrate that pIgR can play a critical role in intestinal defense against the lumen-dwelling protozoan parasite Giardia, a leading cause of diarrheal disease. The receptor was essential for the eradication of Giardia when high luminal IgA levels were required. Clearance of Giardia muris, in which IgA plays a dominant role, was severely compromised in pIgR-deficient mice despite significant fecal IgA output at 10% of normal levels. In contrast, eradication of the human strain Giardia lamblia GS/M, for which adaptive immunity is less IgA dependent in mice, was unaffected by pIgR deficiency, indicating that pIgR had no physiologic role when lower luminal IgA levels were sufficient for parasite elimination. Immune IgA was greatly increased in the serum of pIgR-deficient mice, conferred passive protection against Giardia, and recognized several conserved giardial Ags, including ornithine carbamoyltransferase, arginine deiminase, alpha-enolase, and alpha- and beta-giardins, that are also detected in human giardiasis. Corroborative observations were made in mice lacking the J chain, which is required for pIgR-dependent transepithelial IgA transport. These results, together with prior data on pIgR-mediated immune neutralization of luminal cholera toxin, suggest that pIgR is essential in intestinal defense against pathogenic microbes with high-level and persistent luminal presence.


Assuntos
Giardia , Giardíase/imunologia , Mucosa Intestinal/imunologia , Mucosa Intestinal/parasitologia , Receptores de Imunoglobulina Polimérica/fisiologia , Animais , Antígenos de Protozoários/análise , Antígenos de Protozoários/imunologia , Fezes/química , Giardíase/genética , Imunidade/genética , Imunoglobulina A/análise , Imunoglobulina A/sangue , Imunoglobulina A/metabolismo , Intestinos/imunologia , Intestinos/parasitologia , Camundongos , Camundongos Mutantes , Receptores de Imunoglobulina Polimérica/deficiência , Receptores de Imunoglobulina Polimérica/genética
17.
Genomics ; 79(6): 750-5, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12036287

RESUMO

A novel gene, BIVM (for basic, immunoglobulin-like variable motif-containing), has been identified using an electronic search based on the conservation of short sequence motifs within the variable region of immunoglobulin (Ig) genes. BIVM maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino-acid protein with a pI of 9.1. The 5' untranslated region of BIVM is encoded in two exons; the coding portion is encoded in nine exons. BIVM is tightly linked (41 bp) and in the opposite transcriptional orientation to MGC5302 (also known as KDEL1 and EP58) in human. The ubiquitous expression of BIVM in normal tissues and the presence of a 5' CpG island suggest that BIVM is a housekeeping gene. Characterization of BIVM in representative species demonstrates significant conservation throughout deuterostomes; no sequence with significant identity to BIVM has been detected in proteostomes. However, an unusual gene has been identified in the protozoan pathogen Giardia lamblia that is similar to the core sequence of BIVM, suggesting the possibility of a horizontal gene transfer.


Assuntos
Cromossomos Humanos Par 13 , Genoma Humano , Giardia lamblia/genética , Imunoglobulinas/genética , Proteínas de Protozoários , Vertebrados/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Mapeamento Cromossômico , Sequência Conservada , Transferência Genética Horizontal , Humanos , Camundongos , Dados de Sequência Molecular , Especificidade de Órgãos
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