Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 18 de 18
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Comp Pathol ; 166: 69-86, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30691609

RESUMO

Feline infectious peritonitis (FIP) is an almost invariably fatal feline coronavirus (FCoV)-induced disease thought to arise from a combination of viral mutations and an overexuberant immune response. Natural initial enteric FCoV infection may remain subclinical, or result in mild enteric signs or the development of FIP; cats may also carry the virus systemically with no adverse effect. This study screened mesenteric lymph nodes (MLNs), the presumed first site of FCoV spread from the intestine regardless of viraemia, for changes in the transcription of a panel of innate immune response mediators in response to systemic FCoV infection and with FIP, aiming to identify key pathways triggered by FCoV. Cats with and without FIP, the latter with and without FCoV infection in the MLN, were compared. Higher expression levels in FIP were found for toll-like receptors (TLRs) 2, 4 and 8. These are part of the first line of defence and suggest a response to both viral structural proteins and viral nucleic acid. Expression of genes encoding inflammatory cytokines and chemokines, including interleukin (IL)-1ß, IL-6, IL-15, tumour necrosis factor (TNF)-α, CXCL10, CCL8, interferon (IFN)-α, IFN-ß and IFN-γ, was higher in cats with FIP, consistent with inflammatory pathway activation. Expression of genes encoding transcription factors STAT1 and 2, regulating signalling pathways, particularly of the interferons, was also higher. Among cats without FIP, there were few differences between virus-positive and virus-negative MLNs; however, TLR9 and STAT2 expression were higher with infection, suggesting a direct viral effect. The study provides evidence for TLR involvement in the response to FCoV. This could open up new avenues for therapeutic approaches.


Assuntos
Peritonite Infecciosa Felina/imunologia , Mediadores da Inflamação/imunologia , Linfonodos/imunologia , Animais , Gatos , Coronavirus Felino , Feminino , Masculino , Mesentério/imunologia
2.
J Clin Microbiol ; 45(5): 1469-77, 2007 May.
Artigo em Inglês | MEDLINE | ID: mdl-17376886

RESUMO

A multilocus sequence typing (MLST) scheme was devised for Aspergillus fumigatus. The system involved sequencing seven gene fragments and was applied to a panel of 100 isolates of A. fumigatus from diverse sources. Thirty different sequence types were found among the 100 isolates, and 93% of the isolates differed from the other isolates by only one allele sequence, forming a single clonal cluster as indicated by the eBURST algorithm. The discriminatory power of the MLST method was only 0.93. These results strongly indicate that A. fumigatus is a species of a relatively recent origin, with low levels of sequence dissimilarity. Typing methods based on variable numbers of tandem repeats offer higher levels of strain discrimination. Mating type data for the 100 isolates showed that 71 isolates were type MAT1-2 and 29 isolates were MAT1-1.


Assuntos
Aspergillus fumigatus/classificação , Aspergillus fumigatus/genética , DNA Fúngico/genética , Técnicas de Tipagem Micológica/métodos , Aspergillus fumigatus/patogenicidade , Sequência de Bases , Variação Genética , Filogenia
3.
J Clin Microbiol ; 44(10): 3647-58, 2006 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17021093

RESUMO

We typed 165 Candida albicans isolates from 44 different sources by multilocus sequence typing (MLST) and ABC typing of rRNA genes and determined their homozygosity or heterozygosity at the mating-type-like locus (MTL). The isolates represented pairs or larger sets from individual sources, which allowed the determination of strain diversity within patients. A comparison of replicate sequence data determined a reproducibility threshold for regarding isolates as MLST indistinguishable. For 36 isolate sets, MLST and ABC typing showed indistinguishable or highly related strain types among isolates from different sites or from the same site at different times from each patient. This observation included 11 sets with at least one isolate from a blood culture and a nonsterile site from the same patient. For one patient, strain replacement was evidenced in the form of two sets of isolates from different hospital admissions where the strain types within each set were nearly identical but where the two sets differed both by MLST and ABC typing. MLST therefore confirms the existing view of C. albicans strain carriage. Microvariation, evidenced as small differences between MLST types, resulted in most instances from a loss of heterozygosity at one or more of the sequenced loci. Among isolate sets that showed major strain type differences, some isolates could be excluded as likely examples of handling errors during storage. However, for a minority of isolates, intermittent differences in ABC type for tightly clustered MLST types and intermittent appearances of MTL homozygosity lead us to propose that some C. albicans isolates, or all isolates under yet-to-be-determined conditions, maintain a high level of genetic diversity by mechanisms such as recombination, gene conversion, or chromosomal ploidy change.


Assuntos
Candida albicans/genética , Candida albicans/metabolismo , Técnicas de Tipagem Micológica/métodos , Animais , Feminino , Variação Genética , Perda de Heterozigosidade , Camundongos , Camundongos Endogâmicos BALB C , Filogenia , RNA Fúngico/genética , RNA Ribossômico/genética , Reprodutibilidade dos Testes
4.
J Clin Microbiol ; 41(11): 5265-6, 2003 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-14605179

RESUMO

A panel of 86 different Candida albicans isolates was subjected to multilocus sequence typing (MLST) in two laboratories to obtain sequence data for 10 published housekeeping gene fragments. Analysis of data for all possible combinations of five, six, seven, eight, and nine of the fragments showed that a set comprising the fragments AAT1a, ACC1, ADP1, MPIb, SYA1, VPS13, and ZWF1b was the smallest that yielded 86 unique diploid sequence types for the 86 isolates. This set is recommended for future MLST with C. albicans.


Assuntos
Candida albicans/genética , Genes Fúngicos , Candida albicans/classificação , Sequência Consenso , DNA Fúngico/genética , DNA Fúngico/isolamento & purificação , Diploide , Técnicas Genéticas
5.
Am J Trop Med Hyg ; 65(5): 427-34, 2001 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11716094

RESUMO

The severity of dengue virus infection ranges from mild fever to dengue hemorrhagic fever and shock syndrome. The association of disease severity with virus replication in monocyte-derived macrophages (MDMs) was examined for dengue virus type 2 (DEN-2) isolates from Asia or America. Additionally, we constructed DEN-2 recombinant viruses with substitutions at residue 390 in the envelope glycoprotein (E390) because this residue is linked with the region of virus origin. Comparisons of virus yields of 3 isolates failed to show a correlation with clinical disease. However, the American strain did not replicate as well as the 2 Asian strains. For the recombinant viruses, substitution of Asn (Asian) at E390 with Asp (American) resulted in decreased ability to replicate in MDMs. These results are consistent with the proposal that the lack of association of native American DEN-2 strains with severe disease is linked to reduced ability to replicate in MDMs, and that Asp at E390 may contribute to this reduction.


Assuntos
Vírus da Dengue/crescimento & desenvolvimento , Macrófagos/virologia , Proteínas do Envelope Viral/química , Replicação Viral , Animais , Linhagem Celular , Cricetinae , Vírus da Dengue/genética , Proteínas Recombinantes/química , Relação Estrutura-Atividade , Proteínas do Envelope Viral/fisiologia
6.
J Virol ; 75(20): 9633-43, 2001 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11559795

RESUMO

The protein NS3 of Dengue virus type 2 (DEN-2) is the second largest nonstructural protein specified by the virus and is known to possess multiple enzymatic activities, including a serine proteinase located in the N-terminal region and an NTPase-helicase in the remaining 70% of the protein. The latter region has seven conserved helicase motifs found in all members of the family Flaviviridae. DEN-2 NS3 lacking the proteinase region was synthesized as a fusion protein with glutathione S-transferase in Escherichia coli. The effects of 10 mutations on ATPase and RNA helicase activity were examined. Residues at four sites within enzyme motifs I, II, and VI were substituted, and six sites outside motifs were altered by clustered charged-to-alanine mutagenesis. The mutations were also tested for their effects on virus replication by incorporation into genomic-length cDNA. Two mutations, both in motif I (G198A and K199A) abolished both ATPase and helicase activity. Two further mutations, one in motif VI (R457A,R458A) and the other a clustered charged-to-alanine substitution at R(376)KNGK(380), abolished helicase activity only. No virus was detected for any mutation which prevented helicase activity, demonstrating the requirement of this enzyme for virus replication. The remaining six mutations resulted in various levels of enzyme activities, and four permitted virus replication. For the two nonreplicating viruses encoding clustered changes at R(184)KR(186) and D(436)GEE(439), we propose that the substituted residues are surface located and that the viruses are defective through altered interaction of NS3 with other components of the viral replication complex. Two of the replicating viruses displayed a temperature-sensitive phenotype. One contained a clustered mutation at D(334)EE(336) and grew too poorly for further characterization. However, virus with an M283F substitution in motif II was examined in a temperature shift experiment (33 to 37 degrees C) and showed reduced RNA synthesis at the higher temperature.


Assuntos
Vírus da Dengue/genética , RNA Helicases/metabolismo , Proteínas não Estruturais Virais/genética , Adenosina Trifosfatases/metabolismo , Alanina/genética , Animais , Antígenos Virais/imunologia , Linhagem Celular , Vírus da Dengue/química , Vírus da Dengue/metabolismo , Escherichia coli/genética , Glutationa Transferase/metabolismo , Mutagênese Sítio-Dirigida , Fenótipo , Proteínas Recombinantes de Fusão/metabolismo , Serina Endopeptidases , Temperatura , Proteínas não Estruturais Virais/metabolismo , Replicação Viral
7.
J Cell Sci ; 114(Pt 9): 1743-56, 2001 May.
Artigo em Inglês | MEDLINE | ID: mdl-11309204

RESUMO

The fate of endocytosed membrane proteins and luminal contents is determined by a materials processing system in sorting endosomes. Endosomal retention is a mechanism that traps specific proteins within this compartment, and thereby prevents their recycling. We report that a sorting nexin SNX1, a candidate endosomal retention protein, self-assembles in vitro and in vivo, and has this property in common with its yeast homologue Vps5p. A comparison of SNX1 expressed in bacterial and in mammalian systems and analyzed by size-exclusion chromatography indicates that in cytosol SNX1 tetramers are part of a larger complex with additional proteins. An endosomal retention function would require that SNX1 bind to endosomal membranes, yet the complexes that we analyzed were largely soluble and little SNX1 was found in pellet fractions. Using green fluorescent protein fusions, endocytic compartment markers and fluorescence recovery after photobleaching, we found that there is an equilibrium between free cytoplasmic and early/sorting endosome-bound pools of green fluorescent protein-SNX1. Fluorescence resonance energy transfer indicated that spectral variants of green fluorescent protein-SNX1 were oligomeric in vivo. In cell extracts, these green fluorescent protein-SNX1 oligomers corresponded to tetrameric and larger complexes of green fluorescent protein-SNX1. Using video microscopy, we observed small vesicle docking and tubule budding from large green fluorescent protein-SNX1 coated endosomes, which are features consistent with their role as sorting endosomes. http://www.biologists.com/JCS/movies/jcs2058.html


Assuntos
Proteínas de Transporte/metabolismo , Endossomos/metabolismo , Proteínas de Transporte Vesicular , Sequência de Aminoácidos , Animais , Biopolímeros , Células Cultivadas , Cromatografia em Gel , Endocitose , Proteínas de Fluorescência Verde , Humanos , Proteínas Luminescentes/metabolismo , Microscopia de Fluorescência , Dados de Sequência Molecular , Ligação Proteica , Proteínas Recombinantes de Fusão/metabolismo
8.
Virology ; 260(2): 273-6, 1999 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-10417262

RESUMO

Mushroom bacilliform virus is unique among mycoviruses of the higher fungi in having a genome of positive-sense single-stranded RNA. We have identified a subgenomic mRNA molecule encoding the viral capsid protein in mushroom bacilliform virus-infected mycelium of Agaricus bisporus. Transcription of subgenomic RNA commences at the sequence ACAAAA, 47 nucleotides upstream of the initiating AUG.


Assuntos
Agaricus/virologia , Capsídeo/genética , Genoma Viral , Vírus de RNA/genética , RNA Mensageiro/genética , RNA Viral/genética , Sequência de Bases , Northern Blotting , Conformação de Ácido Nucleico , Fases de Leitura Aberta/genética , RNA Mensageiro/análise , RNA Mensageiro/química , RNA Viral/análise , RNA Viral/química , Homologia de Sequência do Ácido Nucleico , Transcrição Gênica/genética
9.
J Gen Virol ; 79 ( Pt 11): 2631-9, 1998 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9820138

RESUMO

The three flavivirus glycoproteins prM, E and NS1 are formed by post-translational cleavage and are glycosylated by the addition of N-linked glycans. NS1 may form homodimers, whereas E may form homodimers, homotrimers or heterodimers (prM-E). Modification of these processes by mutagenesis of the proteins has the potential to generate viruses that are restricted in growth and are possible vaccine candidates. Using an SV40-based expression system, we previously analysed dimerization and secretion of the NS1 protein of dengue virus type 2 (DEN-2) with mutations in the conserved Cys residues, or within hydrophilic or hydrophobic regions, or at glycosylation sites. In this study, mutations which reduce cleavage at the DEN-2 prM/E signalase cleavage site are described. On the basis of earlier and current results with transient expression, six mutations which reduced NS1 dimerization and two mutations which inhibited prM/E cleavage were analysed individually for their effects on virus growth using a genomic length cDNA clone. Two viruses were obtained that showed reduced growth in cell culture and attenuation of neurovirulence when inoculated into 3-day-old mice. One of these viruses encoded NS1 that lacked the second glycosylation site, the other encoded a Ser --> Ile change at the -3 position of the prM/E cleavage site. A third virus encoding a mutation in NS1 within a hydrophilic region grew as well as the parental virus. No virus was detected for the remaining five mutations.


Assuntos
Vírus da Dengue/crescimento & desenvolvimento , Regulação Viral da Expressão Gênica/fisiologia , Genes Virais , Proteínas não Estruturais Virais/fisiologia , Animais , Vírus da Dengue/genética , Camundongos , Mutagênese Sítio-Dirigida , Mutação Puntual
10.
Virology ; 249(2): 231-7, 1998 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-9791015

RESUMO

Mushroom bacilliform virus (MBV) is often found in cultivated mushrooms (Agaricus bisporus) with La France disease. MBV has a 4-kb ssRNA genome of positive-sense encoding four major open reading frames (ORFs). The arrangement of ORFs at the 5' end of the genome and the deduced amino-acid sequences of two of the putative gene products (protease and RNA-dependent RNA polymerase) show remarkable similarity to some plant viruses, particularly subgroup II luteoviruses. We show that this similarity extends to the translation strategy at the 5' end of the genome, the presence of a genome-linked protein (VPg), and the location of the VPg downstream of the protease motifs in the polypeptide encoded by ORF2.


Assuntos
Agaricus/virologia , Vírus de RNA/genética , RNA Viral/genética , Proteínas do Core Viral/genética , Sequência de Aminoácidos , Sequência de Bases , DNA Complementar/genética , DNA Viral/genética , Genoma Viral , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Fases de Leitura Aberta , Iniciação Traducional da Cadeia Peptídica , Vírus de Plantas/enzimologia , Vírus de Plantas/genética , Biossíntese de Proteínas , Vírus de RNA/enzimologia , Homologia de Sequência de Aminoácidos , Serina Endopeptidases/genética
11.
J Gen Virol ; 79 ( Pt 3): 437-46, 1998 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9519821

RESUMO

A genomic-length cDNA clone corresponding to the RNA of dengue virus type 2 (DEN-2) New Guinea C strain (NGC) was constructed in a low copy number vector. The cloned cDNA was stably propagated in Escherichia coil and designated pDVWS501. RNA transcripts produced in vitro from the cDNA using T7 RNA polymerase yielded infectious virus (MON501) upon electroporation into BHK-21 cells. When compared with parental NGC virus, MON501 replicated to similar levels in Aedes albopictus C6/36 cells and showed similar neurovirulence in suckling mice. In contrast, a second genomic-length cDNA clone (pDVWS310) used as an intermediate in the construction of pDVWS501 produced virus (MON310) that replicated well in C6/36 cells but was not neurovirulent in mice. MON310 contained the prM and E genes of the non-neurovirulent PUO-218 strain in an NGC background. There were seven amino acid differences between the prM and E proteins of MON310 and MON501. The differences were generally conservative, with the exception of E residue 126, which was Glu in MON310 and Lys in MON501. To examine the role of this residue in mouse neurovirulence, substitutions of Glu --> Lys and Lys --> Glu were made in MON310 and MON501, respectively. The properties of these mutants clearly demonstrated that Lys at E residue 126 is a major determinant of DEN-2 mouse neurovirulence.


Assuntos
Encéfalo/virologia , Vírus da Dengue/genética , Vírus da Dengue/patogenicidade , Proteínas do Envelope Viral/genética , Animais , Animais Recém-Nascidos , Sequência de Bases , Células Cultivadas , Insetos , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Reação em Cadeia da Polimerase , RNA Viral/análise , Recombinação Genética , Homologia de Sequência do Ácido Nucleico , Transcrição Gênica , Virulência/genética
13.
Arch Virol ; 141(7): 1231-46, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-8774684

RESUMO

The complete nucleotide sequence of an Australian strain of bean yellow mosaic virus (BYMV-S) has been determined from cloned viral cDNAs. The BYMV-S genome is 9 547 nucleotides in length excluding a poly(A) tail. Computer analysis of the sequence revealed a single long open reading frame (ORF) of 9168 nucleotides, commencing at position 206 and terminating with UAG at position 9374-6. The ORF potentially encodes a polyprotein of 3056 amino acids with a deduced Mr of 347 409. The 5' and 3' untranslated regions are 205 and 174 nucleotides in length respectively. Alignment of the amino acid sequence of the BYMV-S polyprotein with those of other potyviruses identified nine putative proteolytic cleavage sites. The predicted consensus cleavage site of the BYMV NIa protease was found to differ from that described for other potyviruses. Processing of the BYMV polyprotein at the designated proteolytic cleavage sites would result in a typical potyviral genome arrangement. The amino acid sequences of the putative BYMV encoded proteins were compared to the homologous gene products of twelve individual potyviruses to identify overall and specific regions of amino acid sequence homology.


Assuntos
Fabaceae/virologia , Plantas Medicinais , Potyvirus/genética , RNA Viral/química , Sequência de Aminoácidos , Sequência de Bases , Genoma Viral , Dados de Sequência Molecular , Proteínas Virais/metabolismo
14.
Virology ; 202(2): 904-11, 1994 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-8030251

RESUMO

Mushroom bacilliform virus (MBV) is found in association with spherical virus-like particles in cultivated mushrooms (Agaricus bisporus) afflicted with La France disease. MBV possesses a monopartite ssRNA genome of positive sense and differs from the majority of characterized mycoviruses, which contain segmented dsRNA genomes. We have cloned and sequenced the MBV genome and determined that its length is 4009 nucleotides. The MBV genome contains four major and three minor open reading frames and has 5' and 3' noncoding regions of 60 and 250 nucleotides, respectively. The putative RNA-dependent RNA polymerase and the coat protein display homology with corresponding proteins encoded by certain plant viruses, particularly luteoviruses and carmoviruses.


Assuntos
Agaricus , Vírus de Plantas/genética , Vírus de RNA/genética , RNA Polimerase Dependente de RNA/genética , Sequência de Aminoácidos , Sequência de Bases , Capsídeo/genética , Primers do DNA/química , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Fases de Leitura Aberta , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Proteínas Virais/genética
15.
J Gen Virol ; 73 ( Pt 5): 1303-8, 1992 May.
Artigo em Inglês | MEDLINE | ID: mdl-1588327

RESUMO

The nucleotide sequence of RNA 1 of a German isolate of barley yellow mosaic virus has been determined and compared with a Japanese isolate of the same virus. The sequence identity is 93.6% at the nucleotide level and 96% at the amino acid level. Similar values have been found for the polyproteins of the RNA 2 of both isolates (95%). Both isolates show an RNA 1-encoded protein arrangement similar to that of potyviruses such as tobacco etch virus. In contrast, the polyproteins of the small RNAs (RNA 2) do not show such a similarity to the polyproteins of other potyviruses. However, there is a striking difference between the two isolates in the generally highly conserved active site of the RNA-dependent RNA polymerase. The German isolate exactly matches the consensus sequences for previously described potyviral RNA-dependent RNA polymerases, whereas the Japanese isolate does not.


Assuntos
Hordeum/microbiologia , Vírus do Mosaico/genética , Vírus de RNA/genética , RNA Viral/genética , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , DNA de Cadeia Simples/genética , Alemanha , Japão , Dados de Sequência Molecular , Homologia de Sequência do Ácido Nucleico
16.
J Gen Virol ; 72 ( Pt 4): 989-93, 1991 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-2016598

RESUMO

The complete nucleotide sequence of RNA 2 of a German isolate of barley yellow mosaic virus (BaYMV) has been determined. The RNA is 3585 nucleotides in length excluding a 3'-terminal poly(A) tail. The viral plus and minus strands in all three reading frames contained only one large open reading frame which started at positions 156 to 158 and terminated with a UAG codon at positions 2828 to 2830, thus encoding an Mr 98,000 polypeptide. Comparisons with sequences of other viruses revealed that the amino terminus of the polypeptide has homology with the proteolytic domain of the helper component proteinase of several potyviruses. It was determined that the 98K protein is a polyprotein composed of an amino-terminal 28K protein and a 70K protein.


Assuntos
Vírus do Mosaico/genética , RNA Viral/genética , Sequência de Aminoácidos , Sequência de Bases , Códon/genética , Hordeum/microbiologia , Dados de Sequência Molecular , Sondas de Oligonucleotídeos , Fases de Leitura Aberta , Biossíntese de Proteínas , RNA Viral/isolamento & purificação , Homologia de Sequência do Ácido Nucleico , Transcrição Gênica
17.
Plant Mol Biol ; 8(1): 77-85, 1987 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24302526

RESUMO

Near-isogenic cultivars of Hordeum vulgare which differ for the Mlp gene for resistance to Erysiphe graminis f.sp. hordei were inoculated with race 3 of this pathogen and in vitro translation products of mRNA populations compared by 2-dimensional gel electrophoresis and fluorography. This revealed the presence of new mRNA species in infected leaves compared to non-inoculated controls. These new mRNA species were more abundant in resistant leaves than susceptible leaves. A cDNA library was prepared from poly(A)(+)RNA isolated from infected leaves carrying the Mlp gene for resistance (cvMlp). The library was screened by differential hybridization using [(32)P]-labelled cDNA prepared from poly(A)(+)RNA of both control and infected leaves. Six cDNA clones showing greater hybridization to cDNA prepared from infected leaves were selected. These six cDNA clones hybridized to DNA isolated from barley leaves but not to DNA from conidia of the fungus. In Northern blot analysis of RNA from infected leaves the six cDNA clones each hybridized to mRNA species of different size. Translation products for three of the cDNA clones corresponded to infection-related translation products identified on 2-dimensional fluorograms. The cDNA clones were used to study the kinetics of host mRNA induction during infection of the near-isogenic cultivars of barley. The host mRNA species corresponding to the cDNA clones were induced prior to 24 h after inoculation during the primary penetration processes. In addition the mRNAs corresponding to four of the cDNA clones increased to greater amounts in cvMlp than in the near-isogenic susceptible cultivar (cvmlp) over a 2-d period following inoculation. These results suggest that the Mlp gene has a regulatory role in host gene expression resulting in enhanced expression of several host mRNA species following infection by the powdery mildew fungus.

18.
Plant Mol Biol ; 4(5): 275-83, 1985 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24310877

RESUMO

Pairs of susceptible and resistant, near-isogenic cultivars ofHordeum vulgare which differ for the Mla, Mlk and Mlp genes for resistance toErysiphe graminis f. sp.hordei were inoculated with race 3 of this pathogen and patterns of protein synthesis associated with primary infection mapped using pulse-labelling with L-[(35)S]methionine and 2-dimensional electrophoresis. Extraction of proteins with buffer containing detergent revealed the enhanced synthesis of 5 and 8 polypeptides at 25 and 30 h respectively after inoculation of barley carrying the Mla gene (cvMla). The enhanced synthesis of these same polypeptides together with 11 additional polypeptides was observed at 48 h and 72 h after inoculation of barley carrying either the Mlp (cvMlp) or Mlk (cvMlk) genes. The labelling of several major constitutive polypeptides was suppressed in cvMla at 24 h after inoculation; the labelling of six of these polypeptides was also suppressed in both cvMlp and cvMlk but not until 48 and 72 h after inoculation. These results indicate that changes occur in the synthesis of some common polypeptides following infection of cultivars carrying different resistance genes but the timing and extent of these changes varies with the resistance gene in the host.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...