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1.
Nat Commun ; 15(1): 372, 2024 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-38191463

RESUMO

Homing-based gene drives are recently proposed interventions promising the area-wide, species-specific genetic control of harmful insect populations. Here we characterise a first set of gene drives in a tephritid agricultural pest species, the Mediterranean fruit fly Ceratitis capitata (medfly). Our results show that the medfly is highly amenable to homing-based gene drive strategies. By targeting the medfly transformer gene, we also demonstrate how CRISPR-Cas9 gene drive can be coupled to sex conversion, whereby genetic females are transformed into fertile and harmless XX males. Given this unique malleability of sex determination, we modelled gene drive interventions that couple sex conversion and female sterility and found that such approaches could be effective and tolerant of resistant allele selection in the target population. Our results open the door for developing gene drive strains for the population suppression of the medfly and related tephritid pests by co-targeting female reproduction and shifting the reproductive sex ratio towards males. They demonstrate the untapped potential for gene drives to tackle agricultural pests in an environmentally friendly and economical way.


Assuntos
Ceratitis capitata , Tecnologia de Impulso Genético , Feminino , Masculino , Animais , Ceratitis capitata/genética , Agricultura , Alelos , Fontes de Energia Elétrica
2.
Sci Rep ; 13(1): 19866, 2023 11 14.
Artigo em Inglês | MEDLINE | ID: mdl-37964160

RESUMO

Tephritid fruit fly pests pose an increasing threat to the agricultural industry due to their global dispersion and a highly invasive nature. Here we showcase the feasibility of an early-detection SEPARATOR sex sorting approach through using the non-model Tephritid pest, Ceratitis capitata. This system relies on female-only fluorescent marker expression, accomplished through the use of a sex-specific intron of the highly-conserved transformer gene from C. capitata and Anastrepha ludens. The herein characterized strains have 100% desired phenotype outcomes, allowing accurate male-female separation during early development. Overall, we describe an antibiotic and temperature-independent sex-sorting system in C. capitata, which, moving forward, may be implemented in other non-model Tephritid pest species. This strategy can facilitate the establishment of genetic sexing systems with endogenous elements exclusively, which, on a wider scale, can improve pest population control strategies like sterile insect technique.


Assuntos
Ceratitis capitata , Tephritidae , Animais , Masculino , Feminino , Ceratitis capitata/genética , Ceratitis capitata/metabolismo , Fenótipo , Controle Biológico de Vetores/métodos
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