RESUMO
In order to increase the virulence of two commonly used entomopathogens namely Beauveria bassiana and Metarhizium anisopliae for effective mosquito control programs, we developed new combined formulation by the use of immunosuppressive concentration of imidacloprid. Exposure of sublethal concentration (LC10 1.34 ppb) of imidacloprid to second instar larvae for 92 h that differently modulated the larval biochemical markers by decreasing the total protein (20.65% reduction), acetylcholinesterase (35.74% reduction) and increases the ß-carboxylesterase (26.59% increase) and acid phosphatase (30.69% increase) levels while no significant difference was noticed in alkaline phosphatase of C. quinquefasciatus. Further we demonstrated that the imidacloprid exposure brings about reduction in phenoloxidase level (39.48% decrease) and nitric oxide production (37.64% decrease) in larval homogenate over control. The reduction in viability of hemocytes (15.37% decrease) and phagocytic activity of hemocytes (32.18% decrease) was noticed in imidacloprid treatment. Moreover in laboratory condition, exposure of 1 × 107 spores/ml of B. bassiana (984) and M. anisopliae (6060) alone for 92 h cumulative toxicity assay exhibited larval mortality of 36.47% and 47.64% respectively against C. quinquefasciatus. However in the synergistic experimental studies with LC10 of imidacloprid and 1 × 107 spores/ml of B. bassiana or M. anisopliae to the larvae for 92 h cumulative assay brought 60% and 50.59% more insecticidal activity than the respective entomopathogens alone. The substantial increase of larvicidal activity noticed in the synergistic test conditions against larvae of C. quinquefasciatus revealed that the inclusion of sublethal concentration was proved to be useful for effective larval control.
