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1.
C R Acad Sci III ; 314(5): 227-30, 1992.
Artigo em Francês | MEDLINE | ID: mdl-1600463

RESUMO

The secondary vitellin was chromatographically purified and characterized in the crab, Carcinus maenas. It was a lipoglycoprotein of molecular weight 500,000. After dissociation, we observed two major sub units (82,000 and 72,000, respectively), and many lighter minor fractions. These results are compared with those described for some other Crustacea.


Assuntos
Crustáceos/química , Proteínas do Ovo/análise , Animais , Cromatografia em Gel , Imunoeletroforese Bidimensional , Peso Molecular
2.
Biol Bull ; 179(3): 366-373, 1990 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29314955

RESUMO

The polychaete annelids Alvinella pompejana, Alvinella caudata, and Paralvinella grasslei are strictly associated with deep sea hydrothermal vents. Each species possesses an extracellular hemoglobin, Hb, which has been studied and compared to that of a common intertidal polychaete, the lugworm Arenicola marina. The four Hbs exhibit very similar quaternary structures and spectral properties, and only small differences appeared in the gross polypeptide compositions after reduction and sodium dodecyl sulfate denaturation of the native molecules. Conversely, by a comparison of the effects of pH (6.6-7.6) and temperature (10-40{deg}C) on their intrinsic O affinities, Bohr factors, cooperativities, and apparent heats of oxygenation, lugworm Hb can be differentiated from that of the alvinellids, and the Hb of A. pompejana from that of A. caudata. The known biology of the lugworm and a further analysis of the data suggest several hypotheses concerning the in vivo O2 transport function of the alvinellid Hbs, the in vivo blood pH value in the two alvinellid species, their respective range of optimal temperature, and their ability to create a differentiated and stable external microenvironment.

3.
Ann Parasitol Hum Comp ; 63(2): 165-8, 1988.
Artigo em Francês | MEDLINE | ID: mdl-3421646

RESUMO

The blood composition of B. glabrata is analyzed by electrophoresis. Its proteic composition differs in relation with the blood sampling method. Most of the supplementary fractions also have been found in the gut, some proteins possibly come from the hepatopancreas, whereas others could have their origin in the albumen gland. The sampling method must be selected according to these observations.


Assuntos
Biomphalaria/análise , Hemolinfa/análise , Proteínas/análise , Animais , Eletroforese em Gel de Poliacrilamida
4.
Ann Parasitol Hum Comp ; 56(4): 441-8, 1981.
Artigo em Francês | MEDLINE | ID: mdl-7325530

RESUMO

The injection of crude extracts of Sacculina to healthy crabs induces, after a variable lapse of time, qualitative and quantitative changes in their proteinogramm and a delay in their moulting cycle. These changes are the same as those observed in infested specimens. The injection of haemolymph from parasitized crabs gives similar effects. So, the Sacculina could be able to produce effect from afar upon organs not invaded by the roots of the parasite.


Assuntos
Braquiúros/parasitologia , Crustáceos/fisiologia , Hemolinfa/fisiologia , Proteínas/metabolismo , Animais , Braquiúros/fisiologia , Interações Hospedeiro-Parasita
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