Assessment modalities of non-ionizing three-dimensional images for the quantification of facial morphology, symmetry, and appearance in cleft lip and palate: a systematic review.

The use non-ionizing three-dimensional (3D) imaging in cleft lip and palate is an emerging field, but properly designed methods for the assessment of these images are not well established. Therefore, the aim of this study was to review the current literature on the modalities of assessment of non-ionizing 3D images for the quantification of facial morphology, symmetry, and appearance in cleft lip and palate, and to assess the method error of these modalities. A systematic literature search was conducted using MEDLINE (PubMed), Cochrane Central Register of Controlled Trials, Web of Science, and Embase. Cross-sectional studies with prospective or retrospective data collection, using non-ionizing 3D imaging, with a subjective or objective outcome assessment and including at least 10 cleft lip and/or palate patients were considered eligible. Overall, 1767 unique studies were identified and 33 met the inclusion criteria. The images were objectively assessed using elementary measurements and comprehensive statistical methods for superimposition, shape description, and structuring. Subjective assessment was performed using a Likert-type scale or visual analogue scale. It can be concluded that non-ionizing 3D images are widely used in cleft research, but 3D analysis of the images is often methodologically suboptimal. Researchers must fully utilize the content of 3D images to quantify morphology, symmetry, and appearance.

Articulation and oromyofunctional behavior in children seeking orthodontic treatment.

OBJECTIVES: The purpose of this controlled study is to document articulation and oromyofunctional behavior in children seeking orthodontic treatment. In addition, relations between malocclusions, articulation, and oromyofunctional behavior are studied. MATERIALS AND METHODS: The study included 56 children seeking orthodontic treatment. The control group, consisting of 54 subjects matched for age and gender, did not undergo orthodontic intervention. To determine the impact of the occlusion on speech, the Oral Health Impact Profile was used. Speech characteristics, intelligibility and several lip and tongue functions were analyzed using consensus evaluations. RESULTS: A significant impact of the occlusion on speech and more articulation disorders for/s,n,l,t/were found in the subjects seeking orthodontic treatment. Several other phenomena were seen more often in this group, namely more impaired lip positioning during swallowing, impaired tongue function at rest, mouth breathing, open mouth posture, lip sucking/biting, anterior tongue position at rest, and tongue thrust. Moreover, all children with a tongue thrust showed an anterior tongue position at rest. CONCLUSIONS: Children seeking orthodontics have articulatory and oromyofunctional disorders. To what extent a combined orthodontic and logopaedic treatment can result in optimal oral health (i.e. perfect dentofacial unit with perfect articulation) is subject for further multidisciplinary research.

Pulpal regeneration and root development after subcutaneous transplantation of cryopreserved immature teeth in rats.

The purpose of this in vivo study was to investigate revascularization and root growth after autotransplantation of cryopreserved immature teeth. Immature molar teeth were extracted in 4-week-old Wistar rats. In the test group, teeth were cryopreserved for 1 week and transplanted subcutaneously to the abdomen. In the control group, teeth were transplanted subcutaneously immediately after extraction. Material was collected in test and control animals at intervals of 1, 2, 4 and 10 weeks post-transplantation and histological and microradiographical examination was performed. Results showed that during the first weeks after transplantation, pulpal repair was similar in both groups although degenerated pulpal tissue was replaced slower in cryopreserved teeth and some differences in types of hard tissue formation were found between test and control teeth. After 10 weeks, the differences in the regenerated pulpal tissue between cryopreserved and control teeth observed during the first weeks were no longer detectable. No root growth was detected microradiographically 10 weeks after transplantation in any of the transplanted teeth. The presence of dentin-like tissue in the pulp cavity of some autotransplanted cryopreserved teeth, suggests survival of pulpal tissue after cryopreservation.

Apical root resorption of upper incisors during the torquing stage of the tip-edge technique.

The purpose of this study was to investigate whether treatment with the Tip-Edge appliance resulted in more apical root resorption (ARR) of the central and lateral incisors during the torquing (third stage) than the non-torquing phases (first two stages) of orthodontic treatment. The three stages of this orthodontic technique make it possible to examine the amount of root shortening during torque separately from other types of tooth movement. The ARR ratio was calculated in 31 Caucasian patients (20 females, 11 males), after the non-torquing stage of treatment and after the torquing phase, by analysing periapical radiographs taken at the beginning of treatment (T1), before the start of the torquing stage (T2), and at the end of treatment (T3). At T1, the mean age was 13 years 6 months (+/- 3 years 3 months). The mean, standard deviation and range of the ARR ratios were calculated and compared (P < 0.001). T-tests were performed to determine levels of significance, at different stages of treatment, between teeth with and without ARR (ARR ratio = 1). Root shortening at T3 was observed for 70 per cent of the central and 76 per cent of the lateral incisors. At T2, ARR was 48 and 53 per cent, respectively. Compared with T2, 38 per cent of the central incisors and 55 per cent of the lateral incisors showed ARR during the application of torque. At T3, the finding for both was 22 per cent. The mean ARR ratio for the central and lateral incisors was the same after the non-torquing stage of treatment but was significantly different from a tooth with no root resorption. After the torquing stage, the ARR ratio for the central incisors was 0.96 and for lateral incisors 0.92. At the end of treatment, the ratio was 0.89 and 0.85, respectively. This study revealed that both the central and the lateral incisors showed comparable amounts of ARR during the torquing and non-torquing stage of Tip-Edge treatment.

Stability of upper incisor inclination changes in Class II division 2 patients.

The aim of this study was to evaluate the changes in incisor inclination following orthodontic treatment in Class II division 2 patients, and to assess the long-term stability after retention. Dental casts and cephalograms of 61 Class II division 2 patients (31 males and 30 females, mean age 13.4 years) with a closed lip seal were available before (T1), at the end of treatment (T2), and 3.5 years (mean) post-treatment (T3). A method of measuring upper incisor inclination on dental casts was developed for the purpose of the investigation. This involved placing markers on the study casts and then taking radiographs which were measured to establish the upper incisor inclination. Statistical analysis of the data included the calculation of mean values, standard deviation, standard errors, and ranges for each variable. Spearman ranked correlation coefficients were computed for selected model variables and scattergrams plotted. To investigate the influence of the chosen retention therapy, a Mann-Whitney U-test was used. The mean change in incisor inclination during orthodontic treatment was 15.2 degrees. There was a mean relapse of 2.2 degrees between T2 and T3, but this was not considered clinically relevant. The amount of relapse was independent of the type of the retention appliance (Hawley type retainer or lingual retainer). The relationship between long-term stability of the incisor inclination and between the lip line relative to the upper incisors was also investigated, based on cephalometric data. A mean decrease in lip line height of 0.6 mm was seen at T2 and, although the observed change was statistically significant, it would not be clinically relevant.

[Orthodontics based on a new scientific method of evaluating results: "evidence-based"]. / L'orthodontie basé sur une nouvelle méthode scientifique d'evaluation des résultats: l'"evidence-based".

In the orthodontic literature, randomized clinical trials are used to establish standards for the comparison of different clinical approaches. The balancing effect produced in a randomized study makes it possible to attribute the differences in results achieved clearly to treatment and not to variations in the biological characteristics of the patients involved. Recently, most randomized studies in orthodontics have been devoted to testing the hypothesis that some orthodontic appliances exert orthopedic effects that modify dento-facial growth. The authors propose definitions of "orthopedic effects" and of "dento-alveolar changes" and discuss the difficulties encountered in measuring them. They also review clinical studies published over the past 10 years on the correction of Class II malocclusions, on the problems of expansion and also report on Class IIl disorders. In addition they assess the problems associated with these clinical studies such as the ethical requirement of assuring that control groups receive treatment as appropriate for their maladies as that delivered to patients under study, the high cost of carrying them out, the relatively long delay before results can be evaluated, the difficulties of measuring all the different parameters involved, and the need to assemble large enough samples. To sum up, one can conclude that, because of all these impediments, randomized clinical studies have not as yet delivered the hope for benefit of collecting enough "evidence based" information for the orthodontic profession to deliver better treatment to its patients.

A 2-year outcome audit of a versatile orthodontic bone anchor.

This study examined complications leading to, or possibly leading to, treatment failure, related to the use of the orthodontic bone anchor (OBA). The OBA is a potential means of providing absolute anchorage and consists of a base-plate fixed with mono-cortical screws, a neck piercing the soft tissues, and a coronal part with conventional orthodontic hooks, tubes or slots. The investigation took the form of a single centre prospective registry at a supra-regional teaching hospital. Eighteen patients (average age 21 years) had one to four OBAs placed between January 2000 and February 2002. Altogether 35 OBAs were placed. Follow-up took place until April 2004. Reasons for placing the OBAs were noted together with any associated complications during the follow-up period. Twenty-three OBAs have been removed so far, four prematurely (one of them before it was taken into use, due to a change of treatment plan enforced by loss of the contralateral OBA). Nineteen were removed as planned after completion of the intended tooth movements. Common (but minor) complications included granulations, acute gingivitis and gingival recession. Light mobility of the OBA was also noted in some cases, but without clinical repercussions. The OBA can be loaded directly, at the level of the orthodontic archwire or more occlusally. It can be placed at any site at the circumference of the jaws, given good quality and thickness of the bony wall. Conventional biomechanical techniques can be applied. However, the failure rate (premature loss of OBA) of 8.6% is considered high, and has necessitated changes in the hardware and protocol.

[Orthodontics: interface between biology and biomechanics]. / Orthodontie: raakvlak tussen biologie en biomechanica.

As far as craniofacial development is concerned, the orthodontist is mainly interested in growth of the jaws, craniofacial growth patterns and physiologic backgrounds of bone remodelling after tooth displacement. In biomechanics, the principle "action is equal to reaction" is one of the most important issues to deal with. The art in orthodontics is to distribute the reaction forces or to use them on purpose in an attempt to achieve one of the treatment goals. Reaction forces can be neutralized intra-orally. In this respect the location of the center of resistance of the tooth or a group of teeth needs to be defined carefully in order to have a good understanding of the biomechanical principles of tooth displacement. The action forces can be neutralized extra-orally. When no reaction forces on other teeth are applied, there will be no side effects of the reaction forces on other teeth. Research on the biomechanical principles of the headgear does offer some clinical information. Recently in orthodontics, the use of implants has been introduced. It can be used to neutralize reaction forces intra-orally. Moreover, the implants can be used primarily as anchorage during orthodontic tooth movement and later on to replace missing teeth. Recent animal studies at our department have shown that non-axial loading of implants with forces higher than 5 Newton can be used.

Anterior tooth morphology and its effect on torque.

This study was undertaken to determine the variation in crown-root angle (CRA) of the upper incisors and canines as well as the variation in their labial contour. In addition, the influence of the variability of the labial contour and of different bracket heights on torque was evaluated. Proximal radiographs were taken of 160 extracted maxillary teeth (81 incisors and 79 canines). They were digitized and analysed with Jasc Paint Shop Pro 7TM and Mathcad 2001 Professional. The incisal edge, the centre of the cemento-enamel junction (CEJ), and the root apex were digitized to define the crown and root long axis. For all teeth the CRA was measured. At several heights of the labial surface a tangent was determined, enabling measurement of the inclination of the labial surface. The CRA had great variability, ranging from 167 to 195 degrees for the canines (mean value 183 degrees) and from 171 to 195 degrees for the incisors (average 184 degrees). The mean inclinations of the labial surfaces for the incisors varied greatly. Between 4 and 4.5 mm from the incisal edge the standard deviations (SD) were the smallest and between 2 and 4.5 mm from the incisal edge the labial surface angle differed by approximately 10 degrees. For the canines the mean inclinations of the buccal surface also varied. This angle differed by around 10 degrees between 2 and 4.5 mm from the incisal edge, but the SD were much larger than for the incisors. It can be concluded that placement of a bracket on a tooth at varying heights, still within a clinically acceptable range, results in important differences in the amount of root torque.

[Tooth transplantation: an extra dimension in orthodontics]. / Tandtransplantaties: een extra-dimensie in de orthodontie.

In the first part of the presentation, the content of orthodontics has been briefly explained by means of some clinical cases. The main part of the presentation consisted of discussing patient treatment by means of autogenous tooth transplantation with special emphasis on its indications and contra-indications. When a tooth transplantation is carried out properly, a success percentage of 90%, even forty years after treatment, can be expected. This high percentage has been reported in a recent study. The success of tooth transplantation is mainly due to the timing of the transplantation and the non-traumatic handling during surgery. According to the literature, the ideal moment for tooth transplantation is when half to three quarters of the root has been formed. One of the indications for tooth transplantation is the transplantation of a tooth in the cleft of a cleft palate patient. Two cleft palate patients have been presented in which tooth transplantation was carried out after bone grafting in the alveolar cleft. Finally some findings of a recent study, carried out at our department, dealing with cryopreservation of teeth before transplantation, has been presented. According to the findings of this study, cryopreservation, after removal of the pulp tissue does not inhibit the normal ingrowth of new pulp tissue after transplantation.

Location of the centre of resistance of the upper dentition and the nasomaxillary complex. An experimental study.

The purpose of this study was to investigate the initial displacement of the upper dentition and the nasomaxillary complex as a result of different directions of force application, and to determine the initial centres of resistance for both the upper dentition and the nasomaxillary complex. A macerated human skull with a well-aligned upper arch was used as one experimental model and Araldit 208 as a substitute for the periodontal ligament (PDL). Specifically designed 'antenna-headgear' was developed in an attempt to create different points of force application to simulate high-pull and horizontal traction, and orthopaedic force magnitudes of 8 N were applied to the upper dentition and the nasomaxillary complex. Double exposure holography was used to measure the initial displacement. Reproducibility of the technique was tested and found to be reliable. According to the registered fringe patterns, the force application transmitted by the headgear resulted in complex displacement of facial bones. Pure translation of the maxilla and the upper dentition was observed when the force vector passed by in the area of the key-ridge. No obvious difference was found between the centre of resistance of the upper dentition and the nasomaxillary complex. The location of two different centres of resistance could not be confirmed by measuring initial displacements on this macerated human skull.

[Are removable appliances obsolete?]. / Is uitneembare apparatuur verleden tijd?

Removable orthodontic appliances have some limitations compared to fixed appliances. However, a removable appliance can be used during each period of craniofacial growth and dental development. A number of clinical applications for the removable appliance during the mixed and permanent dentition is explained. The use of functional orthopedic appliances and removable retention appliances are also described. It is concluded that the removable appliance can still play a favorable part in the treatment of malocclusions in each developmental stage.

Stability of implants as anchorage for orthopedic traction.

The aim of this animal study was to investigate the stability of osseointegrated fixtures when used as anchorage for orthopedic traction with extreme force magnitude. Three Brånemark fixtures were placed in the left zygomatic arch and three in the right of five adult dogs. An orthopedic nonaxial force of 5 N was applied using an intraoral coil system. The initial displacement immediately after force application was measured by means of speckle interferometry. After 2 months of continuous loading, bone adaptation and mineralization around all implants were analyzed. All the loaded implants were immobile. Significant marginal bone loss at the abutment-fixture interface (<1 mm) was observed around each loaded fixture implant. Bone remodeling was significantly more pronounced at the tension side of the implants, irrespective of fixture length. Radiographical and histological analyses showed bone with normal trabecular pattern around the implants.

Formation of hyodeoxycholic acid from muricholic acid and hyocholic acid by an unidentified gram-positive rod termed HDCA-1 isolated from rat intestinal microflora.

From the rat intestinal microflora we isolated a gram-positive rod, termed HDCA-1, that is a member of a not previously described genomic species and that is able to transform the 3alpha,6beta, 7beta-trihydroxy bile acid beta-muricholic acid into hyodeoxycholic acid (3alpha,6alpha-dihydroxy acid) by dehydroxylation of the 7beta-hydroxy group and epimerization of the 6beta-hydroxy group into a 6alpha-hydroxy group. Other bile acids that were also transformed into hyodeoxycholic acid were hyocholic acid (3alpha, 6alpha,7alpha-trihydroxy acid), alpha-muricholic acid (3alpha,6beta, 7alpha-trihydroxy acid), and omega-muricholic acid (3alpha,6alpha, 7beta-trihydroxy acid). The strain HDCA-1 could not be grown unless a nonconjugated 7-hydroxylated bile acid and an unidentified growth factor produced by a Ruminococcus productus strain that was also isolated from the intestinal microflora were added to the culture medium. Germfree rats selectively associated with the strain HDCA-1 plus a bile acid-deconjugating strain and the growth factor-producing R. productus strain converted beta-muricholic acid almost completely into hyodeoxycholic acid.

Initial orthopaedic displacement compared with longitudinal displacement of the maxilla after a forward force application. An experimental study in dogs.

The aim of this study was to compare the initial orthopaedic displacement of the maxilla in vivo and the longitudinal changes after a forward force application. The sample consisted of five 1-year-old dogs. An anterior force of 5 N on the maxilla was applied by a coil spring system pushing between Brånemark implants and a maxillary splint. The initial displacement of the maxilla after force application was measured by means of speckle interferometry. The longitudinal displacement of the maxilla after a force application during 8 weeks was measured by superimposing standardized lateral cephalograms. The initial, as well as the longitudinal, displacement of the maxilla of the dogs was in a forward direction with some counterclockwise rotation. There was no statistical difference between the initial and longitudinal displacement. The biological response after force application during 8 weeks can be predicted by the initial orthopaedic displacement.

Tauroconjugation of cholic acid stimulates 7 alpha-dehydroxylation by fecal bacteria.

We examined the effect of the type of cholic acid conjugation (taurine-conjugated, glycine-conjugated, or unconjugated cholic acid) on cholic acid 7 alpha-dehydroxylation by intestinal flora. Cholic acid 7 alpha-dehydroxylation in fecal cultures, in cultures of a defined limited flora consisting of a mixture of seven bacterial species isolated from the intestinal tract, and in a binary culture of a 7 alpha-dehydroxylating Clostridium species plus a cholic acid-deconjugating Bacteroides species was studied. We found that tauroconjugation of cholic acid significantly (P < 0.05) increased bacterial 7 alpha-dehydroxylation of cholic acid into deoxycholic acid from 34 to 55% in fecal cultures, from 45 to 60% in defined limited fecal cultures, and from 75 to 100% in binary cultures. Equimolar concentrations of free taurine did not stimulate 7 alpha-dehydroxylation in fecal cultures or in the defined limited flora, but free taurine did stimulate 7 alpha-dehydroxylation in the binary culture. In the binary culture of Clostridium species strain 9/1 plus Bacteroides species strain R1, the minimal flora capable of increased 7 alpha-dehydroxylation of taurocholic acid, strain R1 deconjugated taurine and rapidly reduced it to H2S. Bacteroides species strain R1 did not grow unless taurine or another appropriate reducible sulfur source was present. Clostridium species strain 9/1 did not grow or 7 alpha-dehydroxylate unless H2S or another source of reduced sulfur was present. We conclude that the increased 7 alpha-dehydroxylation of tauroconjugated cholic acid depends on the reduction of taurine to H2S, which is a necessary growth factor for the 7 alpha-dehydroxylating bacteria.

Skeletal and dento-alveolar changes as a result of headgear activator therapy related to different vertical growth patterns.

The purpose of this study is to evaluate the dental and skeletal effect of the headgear activator according to Van Beek (1981). Special emphasis is put on the changes in vertical position of the incisors after treatment. The material for this study consists of pre- and post-treatment cephalograms of 43 girls and 35 boys with severe Class II malocclusions. Changes due to treatment were compared with two control groups: the series of Riolo et al. (1984), and 50 Austrian children with Class II malocclusions who did not receive treatment during the observation period. The headgear activator was found to be an effective treatment tool in the correction of Class II division 1 malocclusions. The observed skeletal changes were limited to minor growth stimulation of the mandible, while an orthopaedic effect on the maxilla could not be established. The dento-alveolar changes due to headgear activator treatment were most obvious in the upper incisor area resulting in an intrusion and a retroclination of these teeth. Moreover, vertical control of the lower anterior facial height in open bite growth patterns was observed in the sample investigated.

[Dental autograft: a supplementary dimension in dental practice]. / L'autogreffe de dents: une dimension supplémentaire dans la pratique dentaire.

This article deals with the possibilities and limitations of autogenous transplantations. In properly selected cases (trauma, missing teeth,...), this procedure might be a valuable alternative treatment. The indications for transplantation, the surgical technique, the recipient site and the choice of the transplant are discussed. The most important parameters which influence the success of a transplantation are: the stage of root formation, the atraumatic removal and insertion of the germ, the final position of the transplant and the post-operative fixation. Clinical examples will illustrate some indications.

Isolation and identification of intestinal steroid-desulfating bacteria from rats and humans.

We isolated 12 strictly anaerobic steroid-3-sulfate-desulfating strains from the intestinal floras of rats and humans. Two strains (S1 and S2) of the same atypical Clostridium species and an atypical Lactobacillus strain (termed R9) were obtained from rats. The human isolates were identified as Eubacterium cylindroides (two strains, H1 and H2), Peptococcus niger (two strains, H4 and H89), and Clostridium clostridiiforme. We also isolated, from different human fecal samples, four strains of phenotypically similar asaccharolytic Bacteroides strains, H6.2a, H6.2b, H65, and H175. Aryl steroid sulfatase activity for estrogen sulfates was present in all isolates. Alkyl steroid sulfatase activity for both 3 alpha- and 3 beta-sulfates was found only in P. niger H4. The same P. niger strain and Clostridium strains S1 and S2 also possessed bile acid sulfatase activity.