Modeling and experiments of O-band transmission over G.654.C optical fiber.

We address the potential application of G.654.C optical fiber for O-band transmission in the wavelength range of 1270 nm to 1330 nm. Fiber samples at the extreme upper end of the cable cutoff manufacturing distribution are chosen for modeling and experimentation. Modeling of multipath interference (MPI) generation in bend conditions representative of cable deployment suggests minimal to negligible penalty and transmission experiments at 100 Gb/s and 400 Gb/s with commercial IMDD transceivers demonstrate longer transmission with increased power margin compared to standard G.652 fiber due to lower O-band attenuation and no adverse impacts from MPI.

Calibration approach for fluorescence lifetime determination for applications using time-gated detection and finite pulse width excitation.

Time-gated techniques are useful for the rapid sampling of excited-state (fluorescence) emission decays in the time domain. Gated detectors coupled with bright, economical, nanosecond-pulsed light sources like flashlamps and nitrogen lasers are an attractive combination for bioanalytical and biomedical applications. Here we present a calibration approach for lifetime determination that is noniterative and that does not assume a negligible instrument response function (i.e., a negligible excitation pulse width) as does most current rapid lifetime determination approaches. Analogous to a transducer-based sensor, signals from fluorophores of known lifetime (0.5-12 ns) serve as calibration references. A fast avalanche photodiode and a GHz-bandwidth digital oscilloscope is used to detect transient emission from reference samples excited using a nitrogen laser. We find that the normalized time-integrated emission signal is proportional to the lifetime, which can be determined with good reproducibility (typically <100 ps) even for data with poor signal-to-noise ratios ( approximately 20). Results are in good agreement with simulations. Additionally, a new time-gating scheme for fluorescence lifetime imaging applications is proposed. In conclusion, a calibration-based approach is a valuable analysis tool for the rapid determination of lifetime in applications using time-gated detection and finite pulse width excitation.