Assuntos
Técnicas de Laboratório Clínico , Diarreia/prevenção & controle , Laboratórios , Técnicas Microbiológicas , Diarreia/microbiologia , Surtos de Doenças/prevenção & controle , Inspeção de Alimentos , Humanos , Testes de Sensibilidade Microbiana , Programas Nacionais de Saúde , Abastecimento de Água/normasAssuntos
Eucariotos/isolamento & purificação , Helmintíase Animal , Helmintos/isolamento & purificação , Mamíferos/parasitologia , Infecções Protozoárias em Animais , Animais , Animais Selvagens , Quirópteros/parasitologia , Reservatórios de Doenças , Filariose/epidemiologia , Filariose/veterinária , Haplorrinos , Malásia , Microfilárias/isolamento & purificação , Nematoides/isolamento & purificação , Ratos , Sciuridae/parasitologiaRESUMO
A new and more reliable way of quantitating lactate dehydrogenase activity, using NADH as a standard in a colorimetric assay, is proposed. Adopting the scheme, we obtained a CV of 2.6% and precision (95% limits) of +/-5.4% for enzyme activities ranging from 110 to 600 U/l. Comparative study against the modified kinetic method of Amador et al. showed excellent correlation, r = 0.99, p less than 0.001. A normal range of 75 to 203 U/l was determined for the proposed scheme.
Assuntos
L-Lactato Desidrogenase/sangue , NAD , Colorimetria/métodos , Estudos de Avaliação como Assunto , Humanos , Indicadores e Reagentes , Metilfenazônio Metossulfato , Sais de TetrazólioRESUMO
A critical assessment of the accuracy and practicability of five alumina-trihydroxyindole methods for the estimation of urinary catecholamines, including our proposed method, was undertaken. The recovery of noradrenaline added to urine obtained by these methods was quantitated against different types of standards, and varied from 22.9 per cent to 104.8 per cent. The major analytical problems (fluorescence suppression and loss of catecholamines during column chromatography) were evaluated and related to the recovery of the respective procedures. The improved method we developed is simple, rapid and reliable. The low fluorescence suppression and column losses of noradrenaline of this method, averaging 2.9 per cent and 5.3 per cent, respectively, resulted in a mean recovery of 92.0 per cent against an external standard. Both the chromatography step and fluorescence development are simple and short, and a batch of ten cases can be completed within 3 hours.
