1.
J Biol Chem
; 257(23): 13911-4, 1982 Dec 10.
Artigo
em Inglês
| MEDLINE
| ID: mdl-6292215
RESUMO
Treatment of leukotriene A4 (LTA4) methyl ester with sodium hydroxide in aqueous methanol at 4 degrees C afforded LTA4, the presence of which was inferred from the UV spectrum of the compound, its rate of reaction with water, and the identity of the hydration products obtained. The half-life of LTA4 in water (pH 7.4, room temperature) was increased from 14 to 500 s by 1 mg/ml of bovine serum albumin. This stabilized (chiral) LTA4 was converted to LTB4 by an epoxide hydrolase activity in the 100,000 x g supernatant fraction from sonified rat basophilic leukemia cells. Neither the ester of LTA4 nor the biologically incorrect enantiomer of LTA4 was metabolized to LTB4 under these conditions.
Assuntos
Ácidos Araquidônicos/metabolismo , Leucemia Experimental/enzimologia , Leucotrieno B4/biossíntese , Animais , Ácidos Araquidônicos/biossíntese , Basófilos/enzimologia , Quimiotaxia , Estabilidade de Medicamentos , Epóxido Hidrolases/metabolismo , Humanos , Cinética , Leucotrieno A4 , Neutrófilos/fisiologia , Ratos , Soroalbumina Bovina
2.
Biochem Biophys Res Commun
; 102(3): 963-9, 1981 Oct 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-7306200