A survey of free-range egg farmers in the United Kingdom: Knowledge, attitudes and practices surrounding antimicrobial use and resistance.

Antimicrobial resistance (AMR) is a significant global challenge affecting human health and attention has been drawn to practices of all stakeholders involved in antimicrobial prescription and administration, including in the livestock sector. This survey of free-range egg farmers (n = 117) was conducted to investigate knowledge, attitudes and practices surrounding antimicrobial use, and identify farmer-led solutions towards responsible antimicrobial use. Most participants proved knowledgeable of AMR and selected treatments based on principles of responsible medicine use. 'Worms' and 'infectious diseases' were the most common reasons for medicine use. Farms with a higher number of poultry houses, younger flock ages at depopulation and farms visited by a vet less than once a year or 3-4 times a year (compared to annually or twice a year) were more likely to select 'ANTIBIOTICS ONLY', as opposed to 'BOTH ANTIBIOTICS AND ANTIPARASITICS' or 'ANTIPARASITICS ONLY' as their most frequently used medicines. Participants from farms with a younger flock age at depopulation, from company-owned farms, and participants purchasing medicines from agricultural merchants instead of veterinary practices were less likely to be taking measures to reduce or replace antimicrobial use. Participants from larger farms and those that had less contact with their vet were less likely to think that they could reduce or replace the amount of antibiotics used. Survey results provided evidence for the important role of veterinarians in guiding antimicrobial stewardship through engagement, collaboration and education. Discussion groups in which farmers share best practices could assist the free-range egg industry in further promoting responsible antimicrobial use.

A survey of dairy cow farmers in the United Kingdom: knowledge, attitudes and practices surrounding antimicrobial use and resistance.

Antimicrobial resistance (AMR) is among the most pressing challenges facing humanity. This survey of dairy cow farmers (n=372) was conducted to explore knowledge, attitudes and practices surrounding veterinary medicine use and to identify farmer-led solutions to reducing, replacing and refining antimicrobial use. Antimicrobials were the most commonly reported veterinary medicines used. Twenty-two per cent of the antibiotics used by dairy farmers contained a highest priority critically important active ingredient. Mastitis was rated as the most important health and welfare challenge and was the most common reason for medicine use. Frequency of veterinary contact was associated with a decrease in the use of antibiotic footbaths, more 'responsible' treatment choices and increased knowledge of AMR. Purchasing medicine from a vet practice rather than elsewhere was associated with an increased likelihood of disposing of waste milk responsibly. These findings highlight the important role of veterinarians in guiding responsible medicine use. Ninety per cent of participants stated that they were trying to reduce their antimicrobial use. Farmers suggested a wide array of alternative treatments and potential interventions for managing herd health. Findings from this project could be used to target education and training surrounding best practice, supporting the important role that farmers play in protecting public health.

State-dependent judgement bias in Drosophila: evidence for evolutionarily primitive affective processes.

Affective states influence decision-making under ambiguity in humans and other animals. Individuals in a negative state tend to interpret ambiguous cues more negatively than individuals in a positive state. We demonstrate that the fruit fly, Drosophila melanogaster, also exhibits state-dependent changes in cue interpretation. Drosophila were trained on a Go/Go task to approach a positive (P) odour associated with a sugar reward and actively avoid a negative (N) odour associated with shock. Trained flies were then either shaken to induce a purported negative state or left undisturbed (control), and given a choice between: air or P; air or N; air or ambiguous odour (1 : 1 blend of P : N). Shaken flies were significantly less likely to approach the ambiguous odour than control flies. This 'judgement bias' may be mediated by changes in neural activity that reflect evolutionarily primitive affective states. We cannot say whether such states are consciously experienced, but use of this model organism's versatile experimental tool kit may facilitate elucidation of their neural and genetic basis.

A Screen-Peck Task for Investigating Cognitive Bias in Laying Hens.

Affect-induced cognitive judgement biases occur in both humans and animals. Animals in a more negative affective state tend to interpret ambiguous cues more negatively than animals in a more positive state and vice versa. Investigating animals' responses to ambiguous cues can therefore be used as a proxy measure of affective state. We investigated laying hens' responses to ambiguous stimuli using a novel cognitive bias task. In the 'screen-peck' task, hens were trained to peck a high/low saturation orange circle presented on a computer screen (positive cue-P) to obtain a mealworm reward, and to not peck when the oppositely saturated orange circle was presented (negative cue-N) to avoid a one second air puff. Ambiguous cues were orange circles of intermediate saturation between the P and N cue (near-positive-NP; middle-M; near-negative-NN), and were unrewarded. Cue pecking showed a clear generalisation curve from P through NP, M, NN to N suggesting that hens were able to associate colour saturation with reward or punishment, and could discriminate between stimuli that were more or less similar to learnt cues. Across six test sessions, there was no evidence for extinction of pecking responses to ambiguous cues. We manipulated affective state by changing temperature during testing to either ~20°C or ~29°C in a repeated measures cross-over design. Hens have been shown to prefer temperatures in the higher range and hence we assumed that exposure to the higher temperature would induce a relatively positive affective state. Hens tested under warmer conditions were significantly more likely to peck the M probe than those tested at cooler temperatures suggesting that increased temperature in the ranges tested here may have some positive effect on hens, inducing a positive cognitive bias.