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1.
Genesis ; 46(12): 738-42, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18850594

RESUMO

We have generated a transgenic mouse line,Tg(Stra8-cre)1Reb (Stra8-cre), which expresses improved Cre recombinase under the control of a 1.4 Kb promoter region of the germ cell-specific stimulated by retinoic acid gene 8 (Stra8). cre is expressed only in males beginning at postnatal day (P)3 in early-stage spermatogonia and is detected through preleptotene-stage spermatocytes. To further define when cre becomes active, we crossed Stra8-cre males with Tg(ACTB-Bgeo/GFP)21Lbe (Z/EG) reporter females and compared the expression of enhanced green fluorescent protein (EGFP) with the protein encoded by the zinc finger and BTB domain containing 16 (Zbtb16) gene, PLZF-a marker for undifferentiated spermatogonia. Co-expression of EGFP is observed in the majority of PLZF+ cells. We also tested recombination efficiency by mating Stra8-cre;Z/EG males and females with wild-type mice and examining EGFP expression in the offspring. Recombination is detected in >95% of Z/EG+ pups born to Stra8-cre;Z/EG fathers but in none of the offspring born to transgenic mothers, a verification that cre is not functional in females. The postnatal, premeiotic, male germ cell-specific activity of Stra8-cre makes this mouse line a unique resource to study testicular germ cell development.


Assuntos
Integrases/metabolismo , Regiões Promotoras Genéticas , Espermatócitos/crescimento & desenvolvimento , Espermatogênese/genética , Proteínas Adaptadoras de Transdução de Sinal , Animais , Diferenciação Celular/genética , Células Cultivadas , Embrião de Mamíferos , Feminino , Genes Reporter/genética , Masculino , Camundongos , Camundongos Transgênicos , Proteínas/metabolismo , Recombinação Genética/genética
2.
Dev Biol ; 278(1): 13-21, 2005 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-15649457

RESUMO

Gametes rely heavily on posttranscriptional control for their differentiation. Translational control, alternative splicing, and alternative processing of the 3' end of mRNAs are all common during spermatogenesis. Tenr, which encodes a highly conserved 72-kDa protein, is expressed solely in germ cells of the testis from the mid-pachytene stage until the elongating spermatid stage. TENR contains a double-stranded RNA binding domain, is localized to the nucleus, and is phylogenetically related to a family of adenosine deaminases involved in RNA editing. We show here that targeted mutation of the Tenr gene causes male sterility. Tenr mutant males have a reduced sperm count, and Tenr-/- sperm show a decrease in motility and an increase in malformed heads. Despite their sterility, some epididymal sperm from Tenr mutants have normal morphology. The ability of Tenr mutant sperm to fertilize zona pellucida-free oocytes and to bind to, but not fertilize, zona pellucida-intact oocytes suggests that the normal-appearing sperm are not able to penetrate the zona pellucida. These data suggest that TENR plays an essential function in spermatid morphogenesis.


Assuntos
Infertilidade Masculina/etiologia , Proteínas Associadas aos Microtúbulos/deficiência , Sequência de Aminoácidos , Animais , Sequência de Bases , DNA/genética , Feminino , Marcação de Genes , Técnicas In Vitro , Infertilidade Masculina/genética , Infertilidade Masculina/metabolismo , Infertilidade Masculina/patologia , Masculino , Camundongos , Camundongos Knockout , Proteínas Associadas aos Microtúbulos/genética , Proteínas Associadas aos Microtúbulos/fisiologia , Dados de Sequência Molecular , Mutação , Biossíntese de Proteínas , Edição de RNA , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/fisiologia , Homologia de Sequência de Aminoácidos , Contagem de Espermatozoides , Motilidade dos Espermatozoides/genética , Motilidade dos Espermatozoides/fisiologia , Interações Espermatozoide-Óvulo/genética , Interações Espermatozoide-Óvulo/fisiologia , Espermatogênese/genética , Espermatogênese/fisiologia , Espermatozoides/anormalidades , Testículo/patologia
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