Association between exposure to airborne trichloramine and health effects in indoor swimming pool workers.

OBJECTIVE: The main objective of this study was to comprehensively investigate the association between trichloramine (TCA) exposure and respiratory health effects in swimming pool workers. METHODOLOGY: In this study, air sampling was performed for TCA concentrations at fixed locations (static measurements) and on individual workers (personal measurements) in six indoor public swimming pools during periods of high swimmer attendance over the winter school break. Health effects were evaluated using questionnaires and fractional exhaled nitric oxide (FENO) tests performed before and after the working day. RESULTS: In these swimming pools, the environmental TCA concentration ranged from 0.11 to 0.88 mg/m³. Worker exposure ranged from 0.05 to 0.72 mg/m³ for personal measurements. Furthermore, in each swimming pool, the average worker exposure to TCA exceeded the recommended occupational exposure limit of 0.35 mg/m³. Personal TCA measurements were consistently lower than static measurements performed around the pool, with a reduction ranging from 21% to 49%. This can be explained by the time that the workers spend in the pool area, office, and break room. The most common respiratory health effects self-reported by the workers were coughing, shortness of breath, and sneezing with prevalence rates of 38%, 37%, and 35%, respectively. This study demonstrated an association between TCA exposure and eye irritation. Analysis of the FENO tests revealed that individuals with preexisting asthma or allergies exhibited sustained FENO elevation. CONCLUSION: The findings suggest that occupational exposure to TCA in indoor swimming pools is a matter of concern. Implementing and improving workplace safety measures is crucial for safeguarding the respiratory health of swimming pool workers.

Estimating airborne trichloramine levels in indoor swimming pools using the well-mixed box model.

Exposure to airborne disinfection by-products, especially trichloramine (TCA), could cause various occupational health effects in indoor swimming pools. However, TCA concentration measurements involve specialized analysis conducted in specific laboratories, which can result in significant costs and time constraints. As an alternative, modeling techniques for estimating exposures are promising in addressing these challenges. This study aims to predict airborne TCA concentrations in indoor swimming pools using a mathematical model, the well-mixed box model, found in the IHMOD tool, freely available on the American Industrial Hygiene Association website. The model's predictions are compared with TCA concentrations measured during various bather load scenarios. The research involved conducting 2-hr successive workplace measurements over 16- to 18-hr periods in four indoor swimming pools in Quebec, Canada. TCA concentrations were estimated using the well-mixed box model, assuming a homogeneous mixing of air within the swimming pool environment. A novel approach was developed to estimate the TCA generation rate from swimming pool water, incorporating the number of swimmers in the model. Average measured concentrations of TCA were 0.24, 0.26, 0.14, and 0.34 mg/m3 for swimming pools 1, 2, 3, and 4, respectively. The ratio of these measured average concentrations to their corresponding predicted values ranged from 0.51 to 1.30, 0.67 to 1.04, 0.57 to 1.14, and 0.68 to 1.49 for the respective swimming pools. In a worst-case scenario simulating the swimming pool at full capacity (maximum bathers allowed), TCA concentrations were estimated as 0.23, 0.36, 0.14, and 0.37 mg/m3 for swimming pools 1, 2, 3, and 4. Recalculated concentrations by adjusting the number of swimmers so as not to exceed the recommended occupational limit concentration of 0.35 mg/m3 gives a maximum number of swimmers of 63 and 335 instead of currently 80 and 424 for swimming pools 2 and 4, respectively. Similarly, for swimming pools 1 and 3, the maximum number of swimmers could be 173 and 398 (instead of the current 160 and 225, respectively). These results demonstrated that the model could be used to estimate and anticipate airborne TCA levels in indoor swimming pools across various scenarios.

Assessment of waste workers occupational risk to microbial agents and cytotoxic effects of mixed contaminants present in the air of waste truck cabin and ventilation filters.

Workers in the waste-processing industry are potentially exposed to high concentrations of biological contaminants, leading to respiratory and digestive problems and skin irritations. However, few data on the exposure of waste collection truck (WCT) drivers are available. The goal was to document the microbial risk of the waste collection truck (WCT) workers while in the vehicle cab. Long-period sampling using the truck air filters (CAF) and short time ambient air sampling in the cab were used. The potential release of microbial particles from CAFs was also investigated since it could contribute to the microbial load of the cabin air. A combination of analytical methods also helped assess the complex mixture of the biological agents. Aspergillus sections Fumigati and Flavi, E. coli, Enterobacter spp. and Legionella spp. were detected in the CAF of trucks collecting three types of waste. The highest levels of bacteria and fungi were found in the CAF from organic WCT. The highest endotoxin concentrations in CAF were 300 EU/cm2. Most of the CAF showed cytotoxic effects on both lung cells and hepatocytes. Only one mycotoxin was detected in a CAF. The maximal concentrations in the ambient WCT air varied according to the type of waste collected. The highest proportion (84%) of the air samples without cytotoxic effects on the lungs cells was for the recyclable material WCTs. The results revealed the potential microbial risk to workers from a complex mixture of bio-contaminants in the cabs of vehicles collecting all types of waste. The sustained cytotoxic effect indicates the potential adverse health-related impact of mixed contaminants (biological and non-biological) for the workers. Overall, this study highlights the benefits of using complementary sampling strategy and combined analytical methods for a the assessment of the microbial risk in work environments and the need to implement protective measures for the workers.Implications: Exposure to microbial agents is a well-known occupational hazard in the waste management sector. No previous study had evaluated the cytotoxicity of ambient air and ventilation filters to document worker exposure to a combination of contaminants during waste collection. This research confirms the usefulness of ventilation filters for long-term characterization of exposure to infectious agents, azole-resistant fungi, coliform bacteria and mycotoxin. Overall, this study highlights the importance of using several sampling and analysis methods for a comprehensive assessment of microbial risk in work environments, as well as the need to implement appropriate protective measures for collection workers.

Complementary sampling strategy and combined analytical methods are helpful in risk assessment.Air filter analysis (long-term sampling) assesses the presence of airborne biological contaminants over a long period.The type of waste collected influences the microbiological hazard of the workers.Waste collection workers are potentially exposed to infectious and mycotoxin-producing fungi.Cytotoxic assays revealed that waste collection workers are potentially.

Full-scale multisampling and empirical modeling of DBPs in water and air of indoor pools.

Disinfection by-products (DBPs) are formed in the water in swimming pools due to reactions between disinfectants (chlorine, bromine, ozone) and the organic matter introduced by bathers and supply water. High concentrations of DBPs are also reported in the air of indoor swimming pools. Based on a robust multisampling program, the levels and variations of DBPs in the air (trichloramine [TCAM] and trihalomethanes [THMs]) and water (THM) were assessed, as well as their precursors (total organic carbon, water temperature, pH, free, and total chlorine) and proxies (CO2 and relative humidity) in four indoor chlorinated swimming pools. High-frequency sampling was conducted during one high-attendance day for each pool. This study focused on parameters that are easy to measure in order to develop models for predicting levels of THMs and TCAM in the air. The results showed that the number of bathers had an important impact on the levels of THMs and TCAM, with a two-to-three-fold increase in air chloroform (up to 110 µg/m3) and a two-to-four-fold increase in TCAM (up to 0.52 mg/m3) shortly after pools opened. The results of this study for the first time showed that CO2 and relative humidity can serve as proxies for monitoring variations in airborne THMs and TCAM. Our results highlight the good predictive capacity of the developed models and their potential for use in day-to-day monitoring. This could help optimize and control DBPs formation in the air of indoor swimming pools and reduce contaminant exposure for both pool employees and users.

Strategies to Assess Occupational Exposure to Airborne Nanoparticles: Systematic Review and Recommendations.

In many industrial sectors, workers are exposed to manufactured or unintentionally emitted airborne nanoparticles (NPs). To develop prevention and enhance knowledge surrounding exposure, it has become crucial to achieve a consensus on how to assess exposure to airborne NPs by inhalation in the workplace. Here, we review the literature presenting recommendations on assessing occupational exposure to NPs. The 23 distinct strategies retained were analyzed in terms of the following points: target NPs, objectives, steps, "measurement strategy" (instruments, physicochemical analysis, and data processing), "contextual information" presented, and "work activity" analysis. The robustness (consistency of information) and practical aspects (detailed methodology) of each strategy were estimated. The objectives and methodological steps varied, as did the measurement techniques. Strategies were essentially based on NPs measurement, but improvements could be made to better account for "contextual information" and "work activity". Based on this review, recommendations for an operational strategy were formulated, integrating the work activity with the measurement to provide a more complete assessment of situations leading to airborne NP exposure. These recommendations can be used with the objective of producing homogeneous exposure data for epidemiological purposes and to help improve prevention strategies.

Comparison of sampling collection strategies for assessing airborne trichloramine levels in indoor swimming pools.

Since 1995, Hery's trichloramine sampling procedure has been widely used to determine trichloramine exposure in indoor swimming pools. This method consists of pumping air at a 1 L/min flow rate for 2 h through a Teflon prefilter and two quartz fiber filters. Modified Hery methods have been reported using different sampling pump flow rates and types of prefilters. It is possible that the prefilter type or sample collection pump flow rate influenced the results of these studies. This study is designed to evaluate the effects of different cassette assemblies and sampling flow rates on the levels of measured trichloramine. Laboratory tests were performed using a trichloramine production setup designed for this study. Workplace measurements were carried out at four indoor swimming pools. Different prefiltering strategies were used: no prefilter, glass prefilter or Teflon prefilter in the sampling cassette, and an original separable prefilter cassette is presented in this study. Laboratory tests indicated that at trichloramine concentrations higher than 1 mg/m3, the percentage of trichloramine captured on the first filter could be less than 75%, which demonstrated possible loss of the material during sampling. An investigation of the prefilter effect on the sampling strategy using different cassette assemblies revealed that using a separable cassette assembly prevented overestimations of trichloramine levels. Furthermore, there were no significant differences between trichloramine concentrations measured at flow rates (from 0.5 to 2 L/min) in swimming pools.

Estimating evaporation rates and contaminant air concentrations due to small spills of non-ideal aqueous organic solvent mixtures in a controlled environment.

Although small spills of non-ideal organic solvent mixtures are ubiquitous undesirable events in occupational settings, the potential risk of exposure associated with such scenarios remains insufficiently investigated. This study aimed to examine the impact of non-ideality on evaporation rates and contaminant air concentrations resulting from small spills of organic solvent mixtures. Evaporation rate constants alphas (α) were experimentally measured for five pure solvents using a gravimetric approach during solvent evaporation tests designed to simulate small spills of solvents. Two equations were used for estimating contaminants' evaporation rates from aqueous mixtures assuming either ideal or non-ideal behavior based on the pure-chemical alpha values. A spill model also known as the well-mixed room model with exponentially decreasing emission rate was used to predict air concentrations during various spill scenarios based on the two sets of estimated evaporation rates. Model predictive performance was evaluated by comparing the estimates against real-time concentrations measured for the same scenarios. Evaluations for 12 binary non-ideal aqueous mixtures found that the estimated evaporation rates accounting for the correction by the activity coefficients of the solvents (median = 0.0318 min-1) were higher than the evaporation rates estimated without the correction factor (median = 0.00632 min-1). Model estimates using the corrected evaporation rates reasonably agreed with the measured values, with a median predicted peak concentrations-to-measured peak concentrations ratio of 0.92 (0.81 to 1.32) and a median difference between the predicted and the measured peak times of -5 min. By contrast, when the non-corrected evaporation rates were used, the median predicted peak concentrations-to-measured peak concentrations ratio was 0.31 (0.08 to 0.75) and the median difference between the predicted and the measured peak times was +33 min. Results from this study demonstrate the importance of considering the non-ideality effect for accurately estimating evaporation rates and contaminant air concentrations generated by solvent mixtures. Moreover, this study is a step further in improving knowledge of modeling exposures related to small spills of organic solvent mixtures.

Microbial contamination and metabolite exposure assessment during waste and recyclable material collection.

Waste workers are exposed to bioaerosols when handling, lifting and dumping garbage. Bioaerosol exposure has been linked to health problems such as asthma, airway irritant symptoms, infectious, gastrointestinal and skin diseases, and cancer. Our objective was to characterize the exposure of urban collectors and drivers to inhalable bioaerosols and to measured the cytotoxic effect of air samples in order to evaluate their health risk. Personal and ambient air sampling were conducted during the summer of 2019. Workers from 12 waste trucks collecting recyclables, organic waste or compost were evaluated. Bacteria and fungi were cultured, molecular biology methods were used to detect microbial indicators, cytotoxic assays were performed and endotoxins and mycotoxins were quantified. Domestic waste collectors were exposed to concentrations of bacteria and endotoxins above the recommended limits, and Aspergillus section Fumigati was detected at critical concentrations in their breathing zones. Cytotoxic effects were observed in many samples, demonstrating the potential health risk for these workers. This study establishes evidence that waste workers are exposed to microbial health risks during collection. It also demonstrates the relevance of cytotoxic assays in documenting the general toxic risk found in air samples. Our results also suggest that exposures differ depending on the type of waste, job title and discharge/unloading locations.

[Occupational medicine, witness to social evolution]. / La médecine du travail : témoin de l'évolution sociale.

Occupational medicine, witness to the industrial and economic evolution of societies, has observed over time the link between diseases and occupational exposures. Nowadays, the transformation of societies brings out new risks, modulated by technical and organizational changes, and our knowledge of their impact on health is constantly evolving. The role of the occupational physician has not changed: he must question and observe in order to know and understand. Its purpose is the same, to prevent workers from falling ill because of their work. It remains essential to pay particular attention to the search for professional factors that may have influenced the disease since the identification and management of the cause is often the best treatment.

La médecine du travail, témoin de l'évolution industrielle et économique des sociétés, s'est intéressée au fil du temps au lien entre les maladies et les expositions professionnelles. De nos jours, la transformation des sociétés fait émerger de nouveaux risques, modulés par les changements techniques et organisationnels, et nos connaissances de leurs impacts sur la santé sont en constante évolution. Le rôle du médecin du travail n'a pas changé : il doit questionner et observer pour connaître et comprendre. Son but reste le même, éviter que les travailleurs ne tombent malades à cause de leur travail. Il demeure primordial d'accorder une attention particulière à la recherche de facteurs professionnels ayant pu influencer la maladie puisque l'identification et la gestion de la cause représentent bien souvent le meilleur traitement.

Predicting first-order evaporation rate constant alpha (α) from small spills of organic solvents in a controlled environment.

Exposures to vapors generated by small spills of organic solvents are common in the occupational hygiene practice. In these scenarios, contaminant mass release is exponentially decreasing, driven by an evaporation rate constant alpha (α). Knowing α is fundamental for adequately modeling peak concentrations and/or short-term exposures that occur and for achieving efficient occupational risk analysis and management. The purpose of this study was to measure alpha experimentally using a gravimetric approach in a controlled environment during solvent evaporation tests designed to simulate small spills of solvents. The effects of several factors on α were evaluated. Equations based on regression models derived from the experimental data were proposed for predicting α. Predictions were externally validated against experimental data. A total of 183 tests was performed. Data analyses found that alpha (α) values increased with vapor pressure, spill surface area-to-spill volume ratio, and air speed across the spill. Larger α were associated with petri dish containers compared to watch glasses. Three regression models were created for predicting α. They had four variables in common, namely vapor pressure, molecular weight, air speed above the liquid, and surface tension of the liquid. The fifth variable was either spill volume, spill surface area, or spill surface area-to-spill volume ratio. The R2 of the regression models were equal to 0.98. External validation showed mean relative errors of -32.9, -32.0, and -25.5%, respectively, with associated standard deviations of the relative errors of 17.7, 33.3, and 26.0%, respectively, and associated R2 of 0.92, 0.65, and 0.87, respectively. The proposed equations can be used for estimating α in exposure scenarios similar to those evaluated in this study. Moreover, these models constitute a step further in the improvement of knowledge on estimating evaporation rates for small spills of organic solvents.

Temporal and spatial variations in the levels of prominent airborne disinfection by-products at four indoor swimming pools.

Exposure to airborne disinfection by-products, especially trichloramine and trichloromethane, may cause various adverse health effects for the workers and users of indoor swimming pools. This study aims to evaluate the spatial and temporal variations in trichloramine and trichloromethane concentrations within and between swimming pools. Workplace measurements were carried out at four indoor swimming pools in Quebec (Canada) during the cold season. To fully represent daily operating conditions, sampling started 2 hr before the swimming pool opened and continued until 2 hr after closing. To quantify trichloramine and trichloromethane concentrations, 304 air samples have been collected. Temperature, humidity, and CO2 were measured-simultaneously every 2 hr. The results showed that both trichloramine and trichloromethane concentrations varied significantly in time. The observed daily variations in trichloramine and trichloromethane concentrations suggest that the common practice of collecting a single 2-hr air sample does not represent daily pool trichloramine and trichloromethane contamination levels and, consequently, does not represent the true exposure and health risks for workers that are present for a full 8-hr shift. This study recommends a new 8-hr sampling strategy or a full-shift strategy using a cassette with three impregnated filters as a valid and cost-effective solution for comparing time-weighted average (TWA) concentrations to permissible trichloramine exposure limits.

Assessment of the Oxidative Potential and Oxidative Burden from Occupational Exposures to Particulate Matter.

Oxidative potential (OP) is a toxicologically relevant metric that integrates features like mass concentration and chemical composition of particulate matter (PM). Although it has been extensively explored as a metric for the characterization of environmental particles, this is still an underexplored application in the occupational field. This study aimed to estimate the OP of particles in two occupational settings from a construction trades school. This characterization also includes the comparison between activities, sampling strategies, and size fractions. Particulate mass concentrations (PM4-Personal, PM4-Area, and PM2.5-Area) and number concentrations were measured during three weeks of welding and construction/bricklaying activities. The OP was assessed by the ascorbate assay (OPAA) using a synthetic respiratory tract lining fluid (RTLF), while the oxidative burden (OBAA) was determined by multiplying the OPAA values with PM concentrations. Median (25th-75th percentiles) of PM mass and number concentrations were 900 (672-1730) µg m-3 and 128 000 (78 000-169 000) particles cm-3 for welding, and 432 (345-530) µg m-3 and 2800 (1700-4400) particles cm-3 for construction. Welding particles, especially from the first week of activities, were also associated with higher redox activity (OPAA: 3.3 (2.3-4.6) ρmol min-1 µg-1; OBAA: 1750 (893-4560) ρmol min-1 m-3) compared to the construction site (OPAA: 1.4 (1.0-1.8) ρmol min-1 µg-1; OBAA: 486 (341-695) ρmol min-1 m-3). The OPAA was independent of the sampling strategy or size fraction. However, driven by the higher PM concentrations, the OBAA from personal samples was higher compared to area samples in the welding shop, suggesting an influence of the sampling strategy on PM concentrations and OBAA. These results demonstrate that important levels of OPAA can be found in occupational settings, especially during welding activities. Furthermore, the OBAA found in both workplaces largely exceeded the levels found in environmental studies. Therefore, measures of OP and OB could be further explored as metrics for exposure assessment to occupational PM, as well as for associations with cardiorespiratory outcomes in future occupational epidemiological studies.

Kinetic time courses of inhaled silver nanoparticles in rats.

Silver nanoparticles (Ag NPs) are priority substances closely monitored by health and safety agencies. Despite their extensive use, some aspects of their toxicokinetics remain to be documented, in particular following inhalation, the predominant route of exposure in the workplace. A same experimental protocol and exposure conditions were reproduced two times (experiments E1 and E2) to document the kinetic time courses of inhaled Ag NPs. Rats were exposed nose-only to 20 nm Ag NPs during 6 h at a target concentration of 15 mg/m3 (E1: 218,341 ± 85,512 particles/cm3; E2, 154,099 ± 5728 particles/cm3). The generated aerosol showed a uniform size distribution of nanoparticle agglomerates with a geometric mean diameter ± SD of 79.1 ± 1.88 nm in E1 and 92.47 ± 2.19 nm in E2. The time courses of elemental silver in the lungs, blood, tissues and excreta were determined over 14 days following the onset of inhalation. Excretion profiles revealed that feces were the dominant excretion route and represented on average (± SD) 5.1 ± 3.4% (E1) and 3.3 ± 2.5% (E2) of the total inhaled exposure dose. The pulmonary kinetic profile was similar in E1 and E2; the highest percentages of the inhaled dose were observed between the end of the 6-h inhalation up to 6-h following the end of exposure, and reached 1.9 ± 1.2% in E1 and 2.5 ± 1.6% in E2. Ag elements found in the GIT followed the trend observed in lungs, with a peak observed at the end of the 6-h inhalation exposure and representing 6.4 ± 4.9% of inhaled dose, confirming a certain ingestion of Ag NPs from the upper respiratory tract. Analysis of the temporal profile of Ag elements in the liver showed two distinct patterns: (i) progressive increase in values with peak at the end of the 6-h inhalation period followed by a progressive decrease; (ii) second increase in values starting at 72 h post-exposure with maximum levels at 168-h followed by a progressive decrease. The temporal profiles of Ag elements in lymphatic nodes, olfactory bulbs, kidneys and spleen also followed a pattern similar to that of the liver. However, concentrations in blood and extrapulmonary organs were much lower than lung concentrations. Overall, results show that only a small percentage of the inhaled dose reached the lungs-most of the dose likely remained in the upper respiratory tract. The kinetic time courses in the gastrointestinal tract and liver showed that part of the inhaled Ag NPs was ingested; lung, blood and extrapulmonary organ profiles also suggest that a small fraction of inhaled Ag NPs progressively reached the systemic circulation by a direct translocation from the respiratory tract.

Environmental and Occupational Short-Term Exposure to Airborne Particles and FEV1 and FVC in Healthy Adults: A Systematic Review and Meta-Analysis.

BACKGROUND: No study has compared the respiratory effects of environmental and occupational particulate exposure in healthy adults. METHODS: We estimated, by a systematic review and meta-analysis, the associations between short term exposures to fine particles (PM2.5 and PM4) and certain parameters of lung function (FEV1 and FVC) in healthy adults. RESULTS: In total, 33 and 14 studies were included in the qualitative synthesis and meta-analyses, respectively. In environmental studies, a 10 µg/m3 increase in PM2.5 was associated with an FEV1 reduction of 7.63 mL (95% CI: -10.62 to -4.63 mL). In occupational studies, an increase of 10 µg/m3 in PM4 was associated with an FEV1 reduction of 0.87 mL (95% CI: -1.36 to -0.37 mL). Similar results were observed with FVC. CONCLUSIONS: Both occupational and environmental short-term exposures to fine particles are associated with reductions in FEV1 and FVC in healthy adults.

Characterization of Occupational Exposures to Engineered Nanoparticles During the Finishing Process of a Hardwood Floor Manufacturing Plant.

Engineered nanomaterials (ENMs) have an enormous economic impact. In the surface coating industry, titanium dioxide (TiO2) and silicon dioxide (SiO2) nanoparticles are commonly incorporated into varnishes, paints, and finishing products. These ENMs are used for UV-active properties and self-cleaning activities, as well as for the durability and resistance they provide. However, several health concerns are associated with the inhalation of some ENMs. In this case study, occupational exposures to engineered nanoparticles were investigated in an industrial hardwood floor manufacturing plant during the finishing task of an automated spraying equipment. A combination of air and surface sampling was carried out during two workdays. Airborne and deposited particles were measured using a combination of real-time and filter-based sampling methods and analyzed by microscopy and spectrometry. Results indicate that the wood floor coating process generates airborne TiO2 and SiO2 nanoparticles which include individual particles in the nanoscale range (lower than 100 nm) and agglomerated particles of several hundred nanometers containing individual nanoparticles. Finishing activities significantly increased total particle number concentrations (45 620 and 117 880 particles cm-3) and surface-specific mass concentrations (154 µm2 cm-3). Concentrations of TiO2 ranged from 13 to 97 µg m-3 for personal measurements and from 36 to 55 µg m-3 for ambient measurements in the finishing location. Characterization of the deposited particles indicated the dispersion of the engineered airborne nanoparticles from the finishing location to the packaging area. Using a multimetric approach, this study shows high evidence that the worker was exposed to engineered TiO2 and SiO2 nanoparticles during the finishing process of the wood floor production facility. In addition, this study indicates that workers outside the finish spray area could be exposed to airborne engineered TiO2 and SiO2 nanoparticles coming from the finishing process.

Modeling occupational exposure to solvent vapors using the Two-Zone (near-field/far-field) model: a literature review.

The Two-Zone model is used in occupational hygiene to predict both near-field and far-field airborne contaminant concentrations. A literature review was carried out on 21 scientific publications in which the Two-Zone model was used to assess occupational exposure to solvent vapors. Data on exposure scenarios, solvents, generation/emission rates, near- and far-field parameters, and model performance were collected and analyzed. Over the 24 exposure scenarios identified, 18 were evaluated under controlled conditions, 5 under normal workplace activities, and 1 was reported based on literature data. The scenarios involved a variety of tasks which consisted, mostly, of cleaning metal parts, spraying solvents onto surfaces, spilling liquids, and filling containers with volatile substances. Twenty-eight different solvents were modeled and the most commonly tested were benzene, toluene, and acetone. Emission rates were considered constant in 16 scenarios, exponentially decreasing in 6 scenarios, and intermittent in 2 scenarios. Four-hundred-and-forty-six (446) predicted-to-measured concentration ratios were calculated across the 21 studies; 441 were obtained in controlled conditions, 4 under normal workplace activities, and 1 was calculated based on the literature data. For controlled studies, the Two-Zone model predictive performance was within a factor of 0.3-3.7 times the measured concentrations with 93% of the values between 0.5 and 2. The model overestimated the measured concentrations in 63% of the evaluations. The median predicted concentration for the near-field was 1.38 vs. 1.02 for the far-field. Results suggest that the model might be a useful tool for predicting occupational exposure to vapors of solvents by providing a conservative approach. Harmonization in model testing strategies and data presentation is needed in future studies to improve the assessment of the predictability of the Two-Zone model. Moreover, this review has provided a database of exposure scenarios, input parameter values, and model predictive performances which can be useful to occupational hygienists in their future modeling activities.

Characterization and Quantification of Ultrafine Particles and Carbonaceous Components from Occupational Exposures to Diesel Particulate Matter in Selected Workplaces.

Questions still exist regarding which indicator better estimates worker's exposure to diesel particulate matter (DPM) and, especially for ultrafine particles (UFP), how exposure levels and the characteristics of the particles vary in workplaces with different exposure conditions. This study aimed to quantify and characterize DPM exposures in three workplaces with different exposure levels: an underground mine, a subway tunnel, and a truck repair workshop. The same sampling strategy was used and included measurements of the particle number concentration (PNC), mass concentration, size distribution, transmission electron microscopy (TEM), and the characterization of carbonaceous fractions. The highest geometric means (GMs) of PNC and elemental carbon (EC) were measured in the mine [134 000 (geometric standard deviation, GSD = 1.5) particles cm-3 and 125 (GSD = 2.1) µg m-3], followed by the tunnel [32 800 (GSD = 1.7) particles cm-3 and 24.7 (GSD = 2.4) µg m-3], and the truck workshop [22 700 (GSD = 1.3) particles cm-3 and 2.7 (GSD = 2.4) µg m-3]. This gradient of exposure was also observed for total carbon (TC) and particulate matter. The TC/EC ratio was 1.4 in the mine, 2.5 in the tunnel and 8.7 in the workshop, indicating important organic carbon interference in the non-mining workplaces. EC and PNC were strongly correlated in the tunnel (r = 0.85; P < 0.01) and the workshop (r = 0.91; P < 0.001), but a moderate correlation was observed in the mine (r = 0.57; P < 0.05). Results from TEM showed individual carbon spheres between 10 and 56.5 nm organized in agglomerates, while results from the size distribution profiles showed bimodal distributions with a larger accumulation mode in the mine (93 nm) compared with the tunnel (39 nm) and the truck workshop (34 nm). In conclusion, the composition of the carbonaceous fraction varies according to the workplace, and can interfere with DPM estimation when TC is used as indicator. Also, the dominance of particles <100 nm in all workplaces, the high levels of PNC measured and the good correlation with EC suggest that UFP exposures should receive more attention on occupational routine measurements and regulations.

Comparison between personal sampling methodologies for evaluating diesel particulate matter exposures in mines: submicron total carbon corrected for the adsorption of vapor-phase organic carbon vs. respirable total carbon.

In the mining industry, personal measurements of elemental and total carbon are frequently used as surrogates of diesel particulate matter (DPM) exposure, and the respirable or submicron fractions are usually measured. However, vapor-phase organic carbon (OC) can be adsorbed in the filters, interfering with total carbon results. This study presents a comparative evaluation between the submicron fraction of DPM concentrations corrected for the adsorption of the vapor-phase OC (dynamic blank), and the respirable fraction of DPM corrected for a field blank. Respirable and submicron fractions of total carbon (TCR and TC1) and elemental carbon (ECR and EC1) concentrations were sampled in parallel, in the workers' breathing zone, in an underground gold mine. A total of 20 full-shift personal samples were taken for each size fraction. Field blanks were collected each day for both the submicron and respirable fractions, while dynamic blank correction was also applied for the submicron fraction. TCR presented a larger and statistically different geometric mean concentration compared to TC1 (98 µg/m3 vs. 72 µg/m3; p = 0.01), while the concentrations of ECR and EC1 were not statistically different (58 µg/m3 vs. 54 µg/m3; p = 0.74). Average TCR/ECR ratio was 1.7, while the TC1/EC1 ratio was 1.3. In addition, 93% of EC had an aerodynamic size lower than 1 µm, while the proportion of TC particles in the submicron fraction was lower (73%). Finally, a similar quantity of OC was found when analyzing the dynamic and field blanks of the filters with the submicron fraction selective size (24 µg and 22 µg, respectively). In conclusion, the correction for the vapor phase OC by the dynamic blank was not a significant correction in our study design compared to the field blank samples. This study suggests that the differences in TC may be explained by the different aerodynamic fractions of DPM collected. In addition, elemental carbon measurements did not seem to be extensively affected by the aerodynamic size of the particles collected.

Diesel engine exhaust exposure in underground mines: Comparison between different surrogates of particulate exposure.

Exposure to diesel particulate matter (DPM) is frequently assessed by measuring indicators of carbon speciation, but these measurements may be affected by organic carbon (OC) interference. Furthermore, there are still questions regarding the reliability of direct-reading instruments (DRI) for measuring DPM, since these instruments are not specific and may be interfered by other aerosol sources. This study aimed to assess DPM exposure in 2 underground mines by filter-based methods and DRI and to assess the relationship between the measures of elemental carbon (EC) and the DRI to verify the association of these instruments to DPM. Filter-based methods of respirable combustible dust (RCD), EC, and total carbon (TC) were used to measure levels of personal and ambient DPM. For ambient measurements, DRI were used to monitor particle number concentration (PNC; PTrak), particle mass concentration (DustTrak DRX and DustTrak 8520), and the submicron fraction of EC (EC1;Airtec). The association between ambient EC and the DRI was assessed by Spearman correlation. Geometric mean concentrations of RCD, respirable TC (TCR) and respirable elemental EC (ECR) were 170 µg/m3, 148 µg/m3, and 83 µg/m3 for personal samples, and 197 µg/m3, 151 µg/m3, and 100 µg/m3 for ambient samples. Personal measurements had higher TCR:ECR ratios compared to ambient samples (1.8 vs. 1.50) and weaker association between ECR and TCR. Among the DRI, the measures of EC1 by the Airtec (ρ = 0.86; P < 0.001) and the respirable particles by the DustTrak 8520 (ρ = 0.74; P < 0.001) showed the strongest association with EC, while PNC showed a weak and non-significant association with EC. In conclusion, this study provided important information about the concentrations of DPM in underground mines by measuring several indicators using filter-based methods and DRI. Among the DRI, the Airtec proved to be a good tool for estimating EC concentrations and, although the DustTrak showed good association with EC, interferences from other aerosol sources should be considered when using this instrument to assess DPM.

Assessment of workers' exposure to microorganisms when using biological degreasing stations.

Biological degreasing stations (BDSs) are used by mechanics. These BDSs use a water-based solution with a microbial degradation process. Occupational exposure during the use of BDSs has not been reported and few studies have identified the bacteria present. The objectives were to measure the concentration of microorganisms during BDSs' use and monitor the bacterial community in the liquid over time. Five mechanical workshops were studied. Six 30-min samples were taken at each workshop over one year. Bioaerosols in the ambient air samples were collected with Andersen impactors near the BDS Bioaerosols in the workers' breathing zone (WBZ) were collected on filters. Fresh bio-degreasing fluids were collected from unopened containers, and used bio-degreasing fluids were collected in the BDS. The results show that the use of BDSs does not seem to increase bioaerosols concentrations in the WBZ (concentrations lower than 480 CFU/m3) and that the bacterial communities (mainly yeasts, Bacillus subtilis and Pseudomonas aeruginosa) in the bio-degreasing fluids change through time and differ from the original community (B. subtilis). This study established that workers using BDSs were exposed to low levels of bioaerosols. No respiratory protection is recommended based on bioaerosols concentrations, but gloves and strict personal hygiene practices are essential.