RESUMO
Two hundred and seventy-seven multidrug resistant clinical isolates [K. pneumoniae, (N = 87); E coli, (N = 30); Salmonella typhimurium (N = 100); P. aeruginosa, (N = 30); S. aureus, (N = 30)] from hospitalized patients specimens, were tested in vitro for sensitivity to Ciprofloxacin. Application of the disk diffusion test and determination of the minimal inhibitory concentration by the microdilution method indicated that, almost all isolates were sensitive to the drug. Overall, S. aureus and P. aeruginosa were the less sensitive organisms. Ciprofloxacin-resistant mutants occurred at frequencies of > or = 10(-5)/CFU.
Assuntos
Ciprofloxacina/farmacologia , Infecções por Enterobacteriaceae/microbiologia , Enterobacteriaceae/efeitos dos fármacos , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Brasil , Resistência Microbiana a Medicamentos/genética , Enterobacteriaceae/genética , Enterobacteriaceae/isolamento & purificação , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Humanos , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/isolamento & purificação , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/isolamento & purificação , Infecções por Salmonella/microbiologia , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/genética , Salmonella typhimurium/isolamento & purificação , Staphylococcus aureus/genética , Staphylococcus aureus/isolamento & purificaçãoRESUMO
A review was made of 138 sputa, 4 bronchial washings and 4 bronchial aspirates from 64 patients with pulmonary tuberculosis. The cytologic findings that may indicate granulomatous involvement were epithelioid cells, giant cells, lymphocytes and a necrotic background. Cell-block preparations stained by the Ziehl-Neelsen technique gave a positivity for acid-fast bacilli similar to that of bacterioscopy. This study emphasizes the potential diagnostic value of inflammatory alterations, even when the main objective of pulmonary cytology is the diagnosis of neoplasia; the finding of epithelioid cells and giant cells in a necrotic background may indicate a specific granulomatous involvement.