1.
Direct pentaplex PCR assay: An adjunct panel for meat species identification in Asian food products.
Food Chem
; 271: 767-772, 2019 Jan 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-30236743
RESUMO
A direct pentaplex PCR assay was developed for the identification of meat from sources other than those declared on the packaging. Species-specific primers were designed, based on the mitochondrial cytochrome oxidase I (COI) gene. The assay amplified specific DNA fragments from dog (230â¯bp), duck (283â¯bp), buffalo (363â¯bp), goat (396â¯bp), and sheep (477â¯bp). The proposed method is capable of identifying target species accurately and is reproducible, sensitive and robust for use with real-world foods and food products. In total, 26 of 117 meat and commercial food products tested were shown to contain DNA from species not declared on the label.