Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 8 de 8
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Magn Reson Imaging Clin N Am ; 29(4): 643-653, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34717851

RESUMO

Neuroimaging with ultra-high field magnets (≥7T) provides superior signal-to-noise, spatial resolution and tissue contrast; but also greater safety concerns, longer scanning times, and increased distortion and field inhomogeneity. Brain and spinal cord anatomic microstructure and function imaged in greater detail offers improved lesion detection, delineation, and characterization. The ongoing development of novel imaging contrasts and translation of cutting-edge sequences will aid more accurate, sensitive, and precise diagnosis, interventional planning, and follow-up for a variety of pathologic conditions.


Assuntos
Imageamento por Ressonância Magnética , Neuroimagem , Encéfalo/diagnóstico por imagem , Criança , Humanos , Medula Espinal/diagnóstico por imagem
2.
NMR Biomed ; 28(6): 633-41, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25871439

RESUMO

Quantification of (31)P NMR spectra is commonly performed using line-fitting techniques with prior knowledge. Currently available time- and frequency-domain analysis software includes AMARES (in jMRUI) and CFIT respectively. Another popular frequency-domain approach is LCModel, which has been successfully used to fit both (1)H and (13)C in vivo NMR spectra. To the best of our knowledge LCModel has not been used to fit (31)P spectra. This study demonstrates the feasibility of using LCModel to quantify in vivo (31)P MR spectra, provided that adequate prior knowledge and LCModel control parameters are used. Both single-voxel and MRSI data are presented, and similar results are obtained with LCModel and with AMARES. This provides a new method for automated, operator-independent analysis of (31)P NMR spectra.


Assuntos
Algoritmos , Encéfalo/metabolismo , Espectroscopia de Ressonância Magnética/métodos , Modelos Biológicos , Compostos de Fósforo/metabolismo , Isótopos de Fósforo/farmacocinética , Animais , Simulação por Computador , Humanos , Imageamento por Ressonância Magnética/métodos , Camundongos , Modelos Químicos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
3.
Magn Reson Med ; 73(1): 13-20, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24436256

RESUMO

PURPOSE: To investigate the effect of Carr-Purcell (CP) pulse trains on transverse relaxation times, T2, of tissue water and metabolites (both noncoupled and J-coupled spins) in the rat brain at 9.4 Tesla (T) using LASER, CP-LASER, and T2ρ-LASER sequences. METHODS: Proton NMR spectra were measured in rat brain in vivo at 9.4T. Spectra were acquired at multiple echo times ranging from 18 to 402 ms. All spectra were analyzed using LCModel with simulated basis sets. Signals of metabolites as a function of echo time were fitted using a mono-exponential function to determine their T2 relaxation times. RESULTS: Measured T2 s for tissue water and all metabolites were significantly longer with CP-LASER and T2ρ-LASER compared with LASER. The T2 increased by a factor of ∼ 1.3 for noncoupled and weakly coupled spins (e.g., N-acetylaspartate and total creatine) and by a factor of ∼ 2 (e.g., glutamine and taurine) to ∼ 4 (e.g., glutamate and myo-inositol) for strongly coupled spins. CONCLUSION: Application of a CP pulse train results in a larger increase in T2 relaxation times for strongly coupled spins than for noncoupled (singlet) and weakly coupled spins. This needs to be taken into account when correcting for T2 relaxation in CP-like sequences such as LASER.


Assuntos
Encéfalo/metabolismo , Interpretação de Imagem Assistida por Computador/métodos , Imageamento por Ressonância Magnética/métodos , Espectroscopia de Ressonância Magnética/métodos , Imagem Molecular/métodos , Algoritmos , Animais , Encéfalo/anatomia & histologia , Masculino , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Processamento de Sinais Assistido por Computador , Distribuição Tecidual
4.
Magn Reson Med ; 72(1): 20-5, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23900976

RESUMO

PURPOSE: The gain in quantification precision that can be expected in human brain (1) H MRS at very high field remains a matter of debate. Here, we investigate this issue using Monte-Carlo simulations. METHODS: Simulated human brain-like (1) H spectra were fitted repeatedly with different noise realizations using LCModel at B0 ranging from 1.5T to 11.7T, assuming a linear increase in signal-to-noise ratio with B0 in the time domain, and assuming a linear increase in linewidth with B0 based on experimental measurements. Average quantification precision (Cramér-Rao lower bound) was then determined for each metabolite as a function of B0 . RESULTS: For singlets, Cramér-Rao lower bounds improved (decreased) by a factor of ∼ B0 as B0 increased, as predicted by theory. For most J-coupled metabolites, Cramér-Rao lower bounds decreased by a factor ranging from B0 to B0 as B0 increased, reflecting additional gains in quantification precision compared to singlets owing to simplification of spectral pattern and reduced overlap. CONCLUSIONS: Quantification precision of (1) H magnetic resonance spectroscopy in human brain continues to improve with B0 up to 11.7T although peak signal-to-noise ratio in the frequency domain levels off above 3T. In most cases, the gain in quantification precision is higher for J-coupled metabolites than for singlets.


Assuntos
Química Encefálica , Espectroscopia de Ressonância Magnética/métodos , Humanos , Campos Magnéticos , Método de Monte Carlo , Razão Sinal-Ruído
5.
Magn Reson Med ; 67(4): 891-7, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21748799

RESUMO

Accurate quantification of (1) H NMR spectra often requires knowledge of the relaxation times to correct for signal losses due to relaxation and saturation. In human brain, T(2) values for singlets such as N-acetylaspartate, creatine, and choline have been reported, but few T(2) values are available for J-coupled spin systems. The purpose of this study was to measure the T(2) relaxation times of J-coupled metabolites in the human occipital lobe using the LASER sequence. Spectra were acquired at multiple echo times and were analyzed with an LCModel using basis sets simulated at each echo time. Separate basis spectra were used for resonances of protons belonging to the same molecule but having very different T(2) values (e.g., two separate basis spectra were used for the singlet and multiplet signal in N-acetylaspartate). The T(2) values for the N-acetylaspartate multiplet (149 ± 12 ms), glutamate (125 ± 10 ms), myo-inositol (139 ± 20 ms), and taurine (196 ± 28 ms) were successfully measured in the human visual cortex at 4 T. These measured T(2) relaxation times have enabled the accurate and absolute quantification of cerebral metabolites at longer echo times.


Assuntos
Espectroscopia de Ressonância Magnética/métodos , Córtex Visual/metabolismo , Adulto , Ácido Aspártico/análogos & derivados , Ácido Aspártico/metabolismo , Colina/metabolismo , Creatina/metabolismo , Ácido Glutâmico/metabolismo , Humanos , Inositol/metabolismo , Processamento de Sinais Assistido por Computador , Taurina/metabolismo
6.
Neurochem Res ; 36(11): 1962-8, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21660589

RESUMO

Determination of oxidative metabolism in the brain using in vivo ¹³C NMR spectroscopy (¹³C MRS) typically requires repeated blood sampling throughout the study to measure blood glucose concentration and fractional enrichment (input function). However, drawing blood from small animals, such as young rats, placed deep inside the magnet is technically difficult due to their small total blood volume. In the present study, a custom-built animal holder enabled temporary removal of the animal from the magnet for blood collection, followed by accurate repositioning in the exact presampling position without degradation of B0 shimming. ¹³C label incorporation into glutamate C4 and C3 positions during a 120 min [1,6-¹³C2] glucose infusion was determined in 28-day-old rats (n = 4) under α-chloralose sedation using localized, direct-detected in vivo ¹³C MRS at 9.4T. The tricarboxylic acid cycle activity rate (V(TCA)) determined using a one-compartment metabolic modeling was 0.67 ± 0.13 µmol/g/min, a value comparable to previous ex vivo studies. This methodology opens the avenue for in vivo measurements of brain metabolic rates using ¹³C MRS in small animals.


Assuntos
Encéfalo/metabolismo , Glucose/metabolismo , Espectroscopia de Ressonância Magnética/métodos , Animais , Isótopos de Carbono , Ciclo do Ácido Cítrico , Espectroscopia de Ressonância Magnética/instrumentação , Oxirredução , Ratos , Ratos Sprague-Dawley
7.
J Magn Reson ; 206(1): 74-80, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20598925

RESUMO

In vivo proton NMR spectroscopy allows non-invasive detection and quantification of a wide range of biochemical compounds in the brain. Higher field strength is generally considered advantageous for spectroscopy due to increased signal-to-noise and increased spectral dispersion. So far (1)H NMR spectra have been reported in the human brain up to 7 T. In this study we show that excellent quality short echo time STEAM and LASER (1)H NMR spectra can be measured in the human brain at 9.4 T. The information content of the human brain spectra appears very similar to that measured in the past decade in rodent brains at the same field strength, in spite of broader linewidth in human brain. Compared to lower fields, the T(1) relaxation times of metabolites were slightly longer while T(2) relaxation values of metabolites were shorter (<100 ms) at 9.4 T. The linewidth of the total creatine (tCr) resonance at 3.03 ppm increased linearly with magnetic field (1.35 Hz/T from 1.5 T to 9.4 T), with a minimum achievable tCr linewidth of around 12.5 Hz at 9.4 T. At very high field, B(0) microsusceptibility effects are the main contributor to the minimum achievable linewidth.


Assuntos
Química Encefálica , Espectroscopia de Ressonância Magnética/métodos , Adulto , Animais , Creatina/análise , Campos Eletromagnéticos , Humanos , Espectroscopia de Ressonância Magnética/instrumentação , Pessoa de Meia-Idade , Lobo Occipital/química , Ratos , Adulto Jovem
8.
Magn Reson Med ; 55(2): 279-86, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16345037

RESUMO

Most in vivo 13C NMR spectroscopy studies in the brain have been performed using 1H decoupling during acquisition. Decoupling imposes significant constraints on the experimental setup (particularly for human studies at high magnetic field) in order to stay within safety limits for power deposition. We show here that incorporation of the 13C label from 13C-labeled glucose into brain amino acids can be monitored accurately using localized 13C NMR spectroscopy without the application of 1H decoupling. Using LCModel quantification with prior knowledge of one-bond and multiple-bond J(CH) coupling constants, the uncertainty on metabolites concentrations was only 35% to 91% higher (depending on the carbon resonance of interest) in undecoupled spectra compared to decoupled spectra in the rat brain at 9.4 Tesla. Although less sensitive, 13C NMR without decoupling dramatically reduces experimental constraints on coil setup and pulse sequence design required to keep power deposition within safety guidelines. This opens the prospect of safely measuring 13C NMR spectra in humans at varied brain locations (not only the occipital lobe) and at very high magnetic fields above 4 Tesla.


Assuntos
Encéfalo/metabolismo , Espectroscopia de Ressonância Magnética/métodos , Animais , Ácido Aspártico/metabolismo , Isótopos de Carbono , Estudos de Viabilidade , Ácido Glutâmico/metabolismo , Glutamina/metabolismo , Método de Monte Carlo , Ratos , Processamento de Sinais Assistido por Computador
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...