Persistent Organic Pollutants in albacore tuna (Thunnus alalunga) from Reunion Island (Southwest Indian Ocean) and South Africa in relation to biological and trophic characteristics.

The contamination of albacore tuna (Thunnus alalunga) by Persistent Organic Pollutants (POPs), namely polychlorinated biphenyls (PCBs) and dichlorodiphenyl-trichloroethane (DDT), was investigated in individuals collected from Reunion Island (RI) and South Africa's (SA) southern coastlines in 2013, in relation to biological parameters and feeding ecology. The results showed lower PCB and DDT concentrations than those previously reported in various tuna species worldwide. A predominance of DDTs over PCBs was revealed, reflecting continuing inputs of DDT. Tuna collected from SA exhibited higher contamination levels than those from RI, related to higher dietary inputs and higher total lipid content. Greater variability in contamination levels and profiles was identified in tuna from RI, explained by a higher diversity of prey and more individualistic foraging behaviour. PCB and DDT contamination levels and profiles varied significantly in tuna from the two investigated areas, probably reflecting exposure to different sources of contamination.

A physiologically based pharmacokinetic model for capreomycin.

The emergence of multidrug-resistant tuberculosis (MDR-TB) has led to a renewed interest in the use of second-line antibiotic agents. Unfortunately, there are currently dearths of information, data, and computational models that can be used to help design rational regimens for administration of these drugs. To help fill this knowledge gap, an exploratory physiologically based pharmacokinetic (PBPK) model, supported by targeted experimental data, was developed to predict the absorption, distribution, metabolism, and excretion (ADME) of the second-line agent capreomycin, a cyclic peptide antibiotic often grouped with the aminoglycoside antibiotics. To account for interindividual variability, Bayesian inference and Monte Carlo methods were used for model calibration, validation, and testing. Along with the predictive PBPK model, the first for an antituberculosis agent, this study provides estimates of various key pharmacokinetic parameter distributions and supports a hypothesized mechanism for capreomycin transport into the kidney.

Virulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutases.

Periplasmic Cu, Zn-cofactored superoxide dismutase (SodC) protects Gram-negative bacteria from exogenous oxidative damage. The virulent Salmonella typhimurium strain ATCC 14028s has been found to contain two discrete periplasmic Cu, Zn-SOD enzymes that are only 57% identical at the amino acid level. SodCI is carried by a cryptic bacteriophage, and SodCII is closely related to the Cu, Zn-superoxide dismutase of Escherichia coli. All Salmonella serotypes appear to carry the sodCII locus, but the phage-associated sodCI gene is found only in certain strains belonging to the most highly pathogenic serotypes. Expression of either sodC locus appears to be enhanced during stationary phase, but only sodCII is regulated by the alternative sigma factor sigmas (RpoS). Mutants lacking both sodC genes are less lethal for mice than mutants possessing either sodC locus alone, indicating that both Cu, Zn-SOD enzymes contribute to Salmonella pathogenicity. The evolutionary acquisition of an additional sodC gene has contributed to the enhanced virulence of selected Salmonella strains.

One-year follow-up after tympanostomy tube insertion for recurrent acute otitis media.

In a prospective study, 126 children were followed for 1 year after the placement of tympanostomy tubes especially for recurrent acute otitis media. About 7 in 10 children remained free of middle ear infection and 1 in 10 showed frequent (i.e. 3 or more) episodes of otorrhea during the follow-up period. However, the most conclusive observation was that the otorrhea episodes were not equally distributed over the different ages: they were markedly more frequent in the group younger then 2 years old, although the prestudy infection rate was similar in all age groups. Furthermore, the incidence of middle ear infection was positively correlated with upper respiratory infection but not with other variables such as prestudy spontaneous perforations, contents of the middle ear, presence of the tube at the end of the study, and swimming habits. It seems that middle ear infection with ventilation tubes in place is influenced by age and upper respiratory infection as it is for acute otitis media in the population as a whole.

Influence of caloric and rotation testing upon blood pressure and pulse rate.

Vestibular stimulation is able to produce neurovegetative effects, which are disagreable and annoying. By an overflow of the vestibular stimulus to the neurovegetative centres changes in blood pressure and pulse rate are more frequently induced than are subjective neurovegetative symptoms as nausea, pallor etc. There is some correlation between both manifestations, but also some independency in occurrence.