Determining global population distribution: methods, applications and data.

Evaluating the total numbers of people at risk from infectious disease in the world requires not just tabular population data, but data that are spatially explicit and global in extent at a moderate resolution. This review describes the basic methods for constructing estimates of global population distribution with attention to recent advances in improving both spatial and temporal resolution. To evaluate the optimal resolution for the study of disease, the native resolution of the data inputs as well as that of the resulting outputs are discussed. Assumptions used to produce different population data sets are also described, with their implications for the study of infectious disease. Lastly, the application of these population data sets in studies to assess disease distribution and health impacts is reviewed. The data described in this review are distributed in the accompanying DVD.

Estimating mortality, morbidity and disability due to malaria among Africa's non-pregnant population.

The contribution of malaria to morbidity and mortality among people in Africa has been a subject of academic interest, political advocacy, and speculation. National statistics for much of sub-Saharan Africa have proved to be an unreliable source of disease-specific morbidity and mortality data. Credible estimates of disease-specific burdens are required for setting global and national priorities for health in order to rationalize the use of limited resources and lobby for financial support. We have taken an empirical approach to defining the limits of Plasmodium falciparum transmission across the continent and interpolated the distributions of projected populations in 1995. By combining a review of the literature on malaria in Africa and models of acquired functional immunity, we have estimated the age-structured rates of the fatal, morbid and disabling sequelae following exposure to malaria infection under different epidemiological conditions.

PIP: This research seeks to estimate mortality, morbidity, and disability due to malaria among Africa's nonpregnant population. It uses an empirical approach to define Plasmodium falciparum transmission limits across the continent. And, the distributions of projected populations in 1995 are interjected. The review of literature on malaria in Africa and models of acquired functional immunity served as a basis for the researchers to predict the age-structured rates of the fatal, morbid and disabling consequences following malaria infection. These estimates were tabulated and analyzed. The results indicated that among populations exposed to stable endemic malaria in sub-Saharan Africa, approximately 987,466 people might have died in 1995 due to malaria infection. On the other hand, over 207.5 million clinical attacks of malaria may have occurred.

A preliminary continental risk map for malaria mortality among African children.

Approaches to global public health are increasingly driven by an understanding of regional patterns of disease-specific mortality and disability. Current estimates of disease risks associated with Plasmodium falciparum in sub-Saharan Africa remain poorly defined. Through the integration of high-resolution population and climate probability models of P. falciparum transmission, geographical information systems have been used to define the spatial limits of populations exposed to the risk of infection in Africa. These estimates were combined with a range of annual malaria-specific mortality rates, derived from a variety of epidemiological approaches, among children aged 0-4 years. The best estimates of malaria-attributable mortality using this approach ranged between 0. 43 million and 0.68 million deaths per annum among an exposed population of approximately 66 million children in 1990. Despite the limitations of modelled transmission and population distributions, these empirical approaches to probabilities of infection risk and epidemiological data on mortality provide a novel approach to present and projected burdens of malaria mortality, as discussed here by Bob Snow, Marlies Craig, Uwe Deichmann and Dave le Sueur.

World population in a grid of spherical quadrilaterals.

"We report on a project that converted subnational population data to a raster of cells on the earth. We note that studies using satellites as collection devices yield results indexed by latitude and longitude. Thus it makes sense to assemble the terrestrial arrangement of people in a compatible manner. This alternative is explored here, using latitude/longitude quadrilaterals as bins for population information.... The results to date of putting world boundary coordinates together with estimates of the number of people are described. The estimated 1994 population of 219 countries, subdivided into 19,032 polygons, has been assigned to over six million five minute by five minute quadrilaterals covering the world."

[The cellulase enzyme system during growth and development of Acanthamoeba castellanii (author's transl)]. / Das Cellulaseenzymsystem während Wachstum und Entwicklung von Acanthamoeba castellanii.

It could be shown that extracts of growing cultures of Acanthamoeba castellanii contained a cellulose degrading system. Reducing sugars are split off by one component of this system at an optimum of pH 4, another enzyme changes the viscosity at an optimum of pH 6, and a third component is a beta-glucosidase with an optimum at pH 3.5. At pH 4 the cellulose degradation products are cellobiose and glucose; at pH 6 higher molecular weight oligosaccharides are produced. During the development from trophozoites to cysts in a nutrient-free medium, the activities of both cellulases decline: Prior to the start of cellulose synthesis only 30%, and in cysts only 10% of the original existing activities are detectable. The biological function of the cellulase enzyme system is discussed together with a consideration of the fact that excystment takes place without digestion of the cyst wall in which the cellulose is deposited.