RESUMO
Objective: Long-term survivors (LS) of non-small cell lung cancer (NSCLC) without driver alterations, displaying an overall survival (OS) of more than 3 years, comprise around 10% of cases in several series treated with chemotherapy. There are classical prognosis factors for these cases [stage, Eastern Cooperative Oncology Group (ECOG), etc.], but more data are required in the literature. In this multi-center study, we focused on LS of advanced NSCLC with OS above 36 months to perform a clinical-pathological and molecular characterization. Methods: In the first step, we conducted a clinical-pathological characterization of the patients. Afterwards, we carried out a genetic analysis by comparing LS to a sample of short-term survivors (SS) (with an OS less than 9 months). We initially used whole-genome RNA-seq to identify differentiating profiles of LS and SS, and later confirmed these with reverse transcription-polymerase chain reaction (RT-PCR) for the rest of the samples. Results: A total of 94 patients were included, who were mainly men, former smokers, having adenocarcinoma (AC)-type NSCLC with an ECOG of 0-1. We obtained an initial differential transcriptome expression, displaying 5 over- and 33 under-expressed genes involved in different pathways: namely, the secretin receptor, surfactant protein, trefoil factor 1 (TFF1), serpin, Ca-channels, and Toll-like receptor (TLRs) families. Finally, RT-PCR analysis of 40 (20 LS/20 SS) samples confirmed that four genes (surfactant proteins and SFTP) were significantly down-regulated in SS compared to LS by using an analysis of covariance (ANCOVA) model: SFTPA1 (P = 0.023), SFTPA2 (P = 0.027), SFTPB (P = 0.02), and SFTPC (P = 0.047). Conclusions: We present a sequential genetic analysis of a sample of NSCLC LS with no driver alterations, obtaining a differential RNA-seq/RT-PCR profile showing an abnormal expression of SF genes.
Assuntos
Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/patologia , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patologia , Idoso , Sobreviventes de Câncer , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Precursores de Proteínas/genética , Proteína A Associada a Surfactante Pulmonar/genética , Proteína C Associada a Surfactante Pulmonar/genética , Proteínas Associadas a Surfactantes Pulmonares/genética , RNA-Seq , Estudos Retrospectivos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Espanha , Análise de SobrevidaRESUMO
Introducción: El consumo de sustancias psicoactivas (SPA) es un problema mundial de Salud pública. Los consumidores suelen utilizar los servicios de urgencias, más que los no Consumidores. Objetivo: Determinar la prevalencia de consumo de seis sustancias psicoactivas en personas entre 18 y 65 años, que acudieron al servicio de urgencias de un hospital Universitario en Bogotá e identificar sus características demográficas. Método: Estudio De corte transversal, con muestreo estratificado por grupos de edad y sexo de pacientes Adultos que acudieron al servicio de urgencias por patologías no psiquiátricas, a quienes Se les aplicó un instrumento que indagaba datos demográficos, médicos, farmacológicos y Toxicológicos. Luego se realizó un análisis toxicológico con el Breath Scan Test, para la detección de alcohol a partir de la exhalación de aire, y el Screeners KO, para la detección en orina de cannabinoides, cocaína, benzodiacepinas, anfetaminas y opiáceos, en las últimas Seis horas. Resultados: Se realizó el análisis en 473 personas. La prevalencia de consumo De cualquier sustancia fue de 7,8%. La sustancia más detectada fue opiáceos, seguida por Alcohol, cannabis, cocaína, benzodiacepinas y anfetaminas. El reporte verbal de consumon las últimas seis horas sólo fue veraz para alcohol. Conclusiones: La prevalencia de Consumo de SPA en la población general de urgencias es alta. De acuerdo con los Resultados, ocho de cada cien personas que consultan a urgencias han consumido alguna SPA en las últimas seis horas...
Introduction: Psychoactive substance abuse is a world public health problem. Drug users tend to use emergency services more than non-users. Objective: To determine the prevalence and demographic characteristics of the use of six substances in people between the ages of 18 and 65, seen at the emergency department of a teaching hospital in Bogotá. Method: Cross sectional study with stratified sampling by age and gender groups of adult patients who were seen at the emergency department (ED). Data collected included demographic, medical, pharmacological, and toxicological information. Toxicological analysis was performed with Scan Breath Test to detect alcohol and KO Screeners to screen urine for use of cannabinoids, cocaine, enzodiazepines, amphetamines, and opiates in the last six hours. Results: Analyses were performed in 473 patients. The prevalence of any substance was 7.8%. The substance most frequently detected was opiates, followed by alcohol, cannabis, cocaine, benzodiazepines, and amphetamines. Conclusions: The prevalence of psychoactive drug use in the general population of the ED is high. According to these results, 8 of out of 100 people who use emergency services have used a psychoactive Substance in the last six hours. The prevalence of self-report was very low compared with The positive results of the toxicology tests...
