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1.
Front Biosci (Landmark Ed) ; 26(3): 431-443, 2021 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-33049676

RESUMO

The communication between neuroendocrine and immune system maintains a bidirectional complex network. Both systems jointly act during a parasite infection to maintain homeostasis and to eliminate such pathogens. Parasites interfere with the synthesis, secretion, metabolism, action, and elimination of endogenous hormones, as well as with the immune system in the host. Here, we aim to address as how parasite colonization disrupts the normal homeostasis of endocrine organs of the host, likely due to the exacerbated immune response, or by the impact of the parasite directly affecting endocrine tissues.


Assuntos
Sistema Endócrino/fisiologia , Interações Hospedeiro-Parasita , Sistema Imunitário/fisiologia , Fenômenos Fisiológicos do Sistema Nervoso , Animais , Humanos
2.
Birth Defects Res ; 112(17): 1326-1340, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32770630

RESUMO

As a result of human socio-economic activity, industrial wastes have increased alarmingly. Plastic pollution is globally distributed across the world due to its properties of buoyancy and durability. Two broad classes of plastic-related chemicals are of critical concern for human health-bisphenol-A or BPA, and additives used in the synthesis of plastics, which are known as phthalates. Our exposure to them is ubiquitous because they are used in the production of materials that we use daily such as polycarbonate plastics, epoxy resins, flooring, automotive parts, medical devices, dental sealants, and children's toys. Since these compounds are not covalently bound to the products, they easily leach from them, leading to high human exposure. Both, BPA and phthalates, are endocrine-disruptor compounds (EDCs) with steroidogenic activity, and can bind to different receptors, such as estrogen, androgen, PPAR-γ, and AhR. These pathways are part of the complex regulatory neuroendocrine network, since its cellular components not only express neuroendocrine receptors, but synthesize and respond to several hormones and other endocrine ligands. On the other hand, the effects of BPA and phthalates on neuroendocrine diseases have been poorly studied and the available data are inconclusive. This can be attributed to the enormous variety of animal models and the different doses used in experiments or levels found in humans. However, what is clear is that exposure to both EDCs during critical life stages induces many changes in the neuroendocrine system of exposed humans that are correlated with different reproductive and neurological diseases.


Assuntos
Disruptores Endócrinos , Plásticos , Animais , Disruptores Endócrinos/toxicidade , Humanos , Microplásticos , Sistemas Neurossecretores/química , Plásticos/toxicidade , Reprodução
3.
Environ Res ; 184: 109381, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32199324

RESUMO

Bisphenol A, a very widespread environmental pollutant and endocrine disruptor compound, can interact with several steroid receptors, particularly with estrogen ones. In different studies, it has observed that the endocrine disruption during critical periods of development can trigger alterations in the immune response during the adult life. Male Wistar rats were exposed indirectly to BPA at a dose of 250 µg/kg day during the perinatal period (from day 5 of pregnancy until day 21 postnatal), At the 60 days of age, the adulthood, animals were infected with larvated eggs of the Toxocara canis, and were sacrificed at 7 days post-infection. Parasitic loads in the lung and in the liver were analyzed by artificial digestion. Furthermore, immune cell subpopulations (macrophages, NK cells, Tγδ, total T cells, T helper, T cytotoxic, and B lymphocytes) present in spleen, peripheral and mesenteric lymph nodes were analyzed by flow cytometry. The expression of Th1 and Th2 cytokines at the splenic level was determined by real-time quantitative PCR. Finally, the titers of specific antibodies against to the parasite were analyzed by ELISA. The BPA treatment administrated in the perinatally stage favors a significant increase of the percentage of Toxocara canis larvae in the lungs and liver in the adulthood. Additionally, the exposure to this compound caused a dramatically decrease in the production of specific antibodies against to this parasite, downregulating together Th2 cytokines (IL-4, IL-5 and IL-13), meanwhile upregulated Th1 cytokines (IFN-γ and TNF-α). Perinatal exposure to BPA affects the performance of the immune response during adult life, modifying both cytokines and antibodies production by these cells, which favors the susceptibility to infections, specifically toxocariosis.


Assuntos
Parasitos , Toxocara canis , Adulto , Animais , Compostos Benzidrílicos/toxicidade , Feminino , Humanos , Masculino , Fenóis , Gravidez , Ratos , Ratos Wistar
4.
Parasit Vectors ; 11(1): 161, 2018 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-29523160

RESUMO

BACKGROUND: We have previously reported that progesterone (P4) has a direct in vitro effect on the scolex evagination and growth of Taenia solium cysticerci. Here, we explored the hypothesis that the P4 direct effect on T. solium might be mediated by a novel steroid-binding parasite protein. METHODS: By way of using immunofluorescent confocal microscopy, flow cytometry analysis, double-dimension electrophoresis analysis, and sequencing the corresponding protein spot, we detected a novel PGRMC in T. solium. Molecular modeling studies accompanied by computer docking using the sequenced protein, together with phylogenetic analysis and sequence alignment clearly demonstrated that T. solium PGRMC is from parasite origin. RESULTS: Our results show that P4 in vitro increases parasite evagination and scolex size. Using immunofluorescent confocal microscopy, we detected that parasite cells showed expression of a P4-binding like protein exclusively located at the cysticercus subtegumental tissue. Presence of the P4-binding protein in cyst cells was also confirmed by flow cytometry. Double-dimension electrophoresis analysis, followed by sequencing the corresponding protein spot, revealed a protein that was previously reported in the T. solium genome belonging to a membrane-associated progesterone receptor component (PGRMC). Molecular modeling studies accompanied by computer docking using the sequenced protein showed that PGRMC is potentially able to bind steroid hormones such as progesterone, estradiol, testosterone and dihydrodrotestosterone with different affinities. Phylogenetic analysis and sequence alignment clearly demonstrated that T. solium PGRMC is related to a steroid-binding protein of Echinoccocus granulosus, both of them being nested within a cluster including similar proteins present in platyhelminths such as Schistocephalus solidus and Schistosoma haematobium. CONCLUSION: Progesterone may directly act upon T. solium cysticerci probably by binding to PGRMC. This research has implications in the field of host-parasite co-evolution as well as the sex-associated susceptibility to this infection. In a more practical matter, present results may contribute to the molecular design of new drugs with anti-parasite actions.


Assuntos
Interações Hospedeiro-Parasita , Progesterona/metabolismo , Receptores de Progesterona/genética , Taenia solium/crescimento & desenvolvimento , Taenia solium/genética , Animais , Eletroforese em Gel Bidimensional , Citometria de Fluxo , Humanos , Microscopia Confocal , Microscopia de Fluorescência , Modelos Moleculares , Simulação de Acoplamento Molecular , Filogenia , Alinhamento de Sequência , Análise de Sequência de DNA , Suínos , Taenia solium/efeitos dos fármacos
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