The Museum of the Department of Public Health and Infectious Diseases, Sapienza University of Rome.

This paper illustrates in detail the birth of the Museum of Public Health of the Sapienza University of Rome, which has been one of the most successful achievements of Prof Carmine Melino in the last few years of his academic career. Backed by a very thin group of enthousiastic coworkers and colleagues, he recuperated all the instruments which had been used by the research groups active since 1880 at the former Institute of Hygiene, to which he added samples of the different pieces of laboratory furniture, ancient reagents, etc. The goal was not to simply collect, restore and maintain the documents of the Institute's past, but to rebuild a vintage laboratory, as it was inhabited by the hygienists of the past and to describe the kinds of research being performed during a period more than a century long. Beginning from the days when Hygiene became a scientific discipline, he tried to demonstrate that only the transformation of Hygiene into an experimental discipline made it possible the numberless achievements, including the improvements of the environmental conditions, the reduction of infectious diseases and the successful fight against the chronic, degenerative diseases of the present times.

[The scientific contributions by the Roman School of Hygiene on the microbiological quality of the surface waters of Rome and her County from 1890 to 2010. A systemic review]. / La produzione scientifica della Scuola Romana di Igiene sulla qualità microbiologica dei corpi idrici superficiali di Roma e Provincia dal 1890 al 2010. Una review sistematica.

Research on quality of surface waters has been performed also in Italy during the development of the large urban areas, and in Rome this has been the duty of the Istituto di Igiene of the Sapienza University since 1890. Using MedLine--and also traditional consultation for papers printed before 1968--we identified 100 articles printed in the period 1890-2010. Thirty of them met the inclusion criteria (to have been written by researchers belonging to the Rome universities and to contain microbiological informations about the surface waters of Rome). The majority of papers identified (46.6%) were produced during the years Sixties and Seventies of the 20th century, and 30% in the twenty years to follow (1980-1999). The most frequent microbiological descriptors were "Total coliforms" and "Streptococci". The waterbodies most investigated were the Tiber river and the coastal waters around Fiumicino, where the Tiber flows into the Tyrrhenian sea. The quality of surface waters has always been a central interest of the research performed by the Hygienists of the Roman School. The good quality of the past research and the renovated interest of International Organizations and of the European Union should encourage the public health researchers toward a strategic field of investigation which has strong interconnections with the protection of the individual and community health and also with the protection of the environment.

Innate immunity in wooless lamb to larvae of Amblyomma cajennense tick (Fabricius, 1787) (Acari: Ixodidae).

The Cayenne tick Amblyomma cajennense infests preferably horses in its adult form but other mammal species in its immature stages and is the main vector of Rickettsia rickettsii, the Brazilian spotted fever pathogen. As wooless lambs are often raised on pasture together with horses, an experiment was performed to investigate their possible acquisition of resistance to A. cajennense after experimental infestations. Seven naïve wooless lambs were infested thrice at 60 days interval with immature and adult instars of A. cajennense from a laboratory colony, the tick biotic potential being determined and biopsies of tick bite lesions taken to investigate the inflammatory cell component. Nearly 100% of larvae died in all infestations, while nymphs and adults fed normally throughout re-infestations. Microscopic features of adult tick bite lesions revealed predominance of neutrophils (38%) and eosinophils (36.8%), respectively, in the first and second infestations. In the third infestation, 43.6% of MN cells were found and about 31% of eosinophils. On the other hand, nymph bite lesions revealed in all infestations a predominance of eosinophils, increasing from 36% in the first infestation to 50.5% in the third one. It is concluded that wooless lambs present remarkable innate resistance against larvae of A. cajennense, but marked susceptibility to the other tick instars despite the migration of great number of eosinophils to the tick lesion.

Localization of antigenic sites in unfed nymphs of Amblyomma triste Koch 1844 (Acari: Ixodidae) ticks by immunohistochemistry.

The reactivity of sera collected from guinea pigs after three infestations with Amblyomma triste nymphs on histological sections of the same tick species was investigated through immunohistochemistry to identify potential target cells and tissues. Six guinea pigs were infested thrice, at 30 day intervals, with 30 nymphs of A. triste per animal per infestation. Blood samples were collected from the guinea pigs 15 days after each infestation for serum separation; normal serum was obtained before the first infestation as control. Unfed A. triste nymphs' histological sections were submitted to indirect immunohistochemistry technique by using normal or hyperimmune guinea pig serum as primary antibody and a goat IgG-alkaline phosphatase-APase conjugate as secondary antibody. A weak to moderate APase activity was observed in cells of salivary glands, midgut and haemolymph of unfed nymphs incubated with hyperimmune serum.

Efficacy of the Bm86 antigen against immature instars and adults of the dog tick Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae).

The Bm86 antigen has been used to control ticks of the Boophilus genera in integrated programs that also include the use of acaricides. Because of recent phylogenetic studies have lead to the inclusion of all Boophilus species within the Rhipicephalus genera, we aimed to investigate the efficacy of the Bm86 antigen on the biotic potential of Rhipicephalus sanguineus. Domestic dogs were vaccinated with Bm86 and challenged with the three instars of R. sanguineus. Male and female mongrel dogs were divided into two groups of four animals each, comprising non-vaccinated and vaccinated animals. Immunized dogs were given two doses of an experimental formulation containing 50mug of recombinant Bm86, at 21 days interval while the other group was given placebo, consisting of the same preparation without Bm86. Each dog was challenged 21 days after the last dose with 250 larvae, 100 nymphs and 55 adults (25 females and 30 males) released inside feeding chambers (one per instar) glued to their shaved flank. The effect of the vaccination was evaluated by determining biological parameters of ticks including the yield rates of larvae, nymphs and adult females. Adult females engorged weight, egg mass weight, efficiency rate of conversion to eggs (ERCE) and hatchability. In addition, sera were collected from dogs at 0, 21, 36, 45 and 75 days after the vaccination and used for the detection of specific antibodies by ELISA. Collection rates of larvae, nymphs and adult females fed on vaccinated dogs were significantly (p<0.05) reduced by 38%, 29% and 31%, respectively, as compared with non-vaccinated controls. Significant reductions were also observed in weight of engorged females and egg mass, in ERCE, but not in the hatch rate of ticks fed on immunized dogs. ELISA data revealed a marked and significant increase in optical densities of sera from vaccinated animals after the second dose of Bm86. We concluded that the Bm86 antigen used as a vaccine for dogs reduced the viability and biotic potential of the R. sanguineus.

[The association of asbestos and cigarette smoke in lung cancer. Note 2]. / L'associaione asbesto-fumo di sigaretta nel cancro polmona- re. Nota 2.

This work follows up the previous edited in the year 2004, and deals with the still serious and discussed problem of work risks even though the use of asbestos has been forbidden since 10 years, and the tobacco's smoke since one year. The Authors discuss the question related to the genetic point of view as a first and multiplying action caused at the some time by the tobacco's smoke and asbestos dust in the working places. This work report an up-to-date list of law about environmental prevention.

Leptin secretion in horses: effects of dexamethasone, gender, and testosterone.

Five experiments were performed to evaluate the effects of dexamethasone (DEX), gender, and testosterone on plasma leptin concentrations in horses. In experiment 1, plasma leptin, insulin, glucose, and IGF-1 concentrations were increased (P < 0.01) in stallions following five daily injections of DEX (125 microg/kg BW). In experiment 2, leptin concentrations increased (P < 0.01) in mares, geldings, and stallions following a single injection of DEX, and the response was greater (P < 0.01) in mares and geldings than in stallions. The gender effect was confounded by differences in body condition scores and diet; however, based on stepwise regression analysis, both BCS and gender were significant sources of variation in the best fit model for pre-DEX leptin concentrations (R(2) = 0.65) and for maximum leptin response to DEX (R(2) = 0.75). In experiment 3, in which mares and stallions were pair-matched based on age and body condition and fed similar diets, mares again had higher (P < 0.01) leptin concentrations than stallions after DEX treatment as used in experiment 2. In experiment 4, there was no difference (P > 0.1) in plasma leptin response in mares following four single-injection doses of DEX from 15.6 to 125 microg/kg BW. In experiment 5, treatment of mares with testosterone propionate every other day for 5 days did not alter (P > 0.1) plasma leptin concentrations or the leptin response to DEX. In conclusion, multiple injections of DEX increase leptin concentrations in stallions, as does a single injection in mares (as low as 15.6 microg/kg BW), geldings and stallions. The greater leptin levels observed in mares and geldings relative to stallions were due partially to their greater body condition and partially to the presence of hyperleptinemic individuals; however, even after accounting for body condition and diet, mares still had greater leptin concentrations than stallions after DEX administration. Elevation of testosterone levels in mares for approximately 10 days did not alter leptin concentrations in mares.

[Topical notes about work diseases rules]. / Nuovo elenco delle malattie professionali a denuncia obbligatoria.

The Authors explain the text of the Decree April 27 2004 related to the new list of work diseases to be reported as by law enacted, instead of the previous Decree April 18 1973. The Decree follows up the latest UE Recommendation 2003/670 in order to bring up to date the European list of work diseases. The Authors examine several topical points of this decree, wishing for Italy a new up to date list for work diseases as from the last DPR April 13, 1994 no 336.

Characterization of the nociceptin receptor (ORL-1) agonist, Ro64-6198, in tests of anxiety across multiple species.

RATIONALE: Previous studies have demonstrated behaviors indicative of anxiolysis in rats pretreated with the nociceptin receptor (opioid receptor like-1, ORL-1) agonist, Ro64-6198. OBJECTIVES: The aim of this study was to examine the effects of Ro64-6198 in anxiety models across three species: rat, guinea pig, and mouse. In addition, the receptor specificity of Ro64-6198 was studied, using the ORL-1 receptor antagonist, J-113397, and ORL-1 receptor knockout (KO) mice. Finally, neurological studies examined potential side effects of Ro64-6198 in the rat and mouse. RESULTS: Ro64-6198 (3-10 mg/kg) increased punished responding in a rat conditioned lick suppression test similarly to chlordiazepoxide (6 mg/kg). This effect of Ro64-6198 was attenuated by J-113397 (10 mg/kg), but not the mu opioid antagonist, naltrexone (3 mg/kg). In addition, Ro64-6198 (1-3 mg/kg) reduced isolation-induced vocalizations in rat and guinea pig pups. Ro64-6198 (3 mg/kg) increased the proportion of punished responding in a mouse Geller-Seifter test in wild-type (WT) but not ORL-1 KO mice, whereas diazepam (1-5.6 mg/kg) was effective in both genotypes. In rats, Ro64-6198 reduced locomotor activity (LMA) and body temperature and impaired rotarod, beam walking, and fixed-ratio (FR) performance at doses of 10-30 mg/kg, i.e., three to ten times higher than an anxiolytic dose. In WT mice, Ro64-6198 (3-10 mg/kg) reduced LMA and rotarod performance, body temperature, and FR responding, but these same measures were unaffected in ORL-1 KO mice. Haloperidol (0.3-3 mg/kg) reduced these measures to a similar extent in both genotypes. These studies confirm the potent, ORL-1 receptor-mediated, anxiolytic-like effects of Ro64-6198, extending the findings across three species. Ro64-6198 has target-based side effects, although the magnitude of these effects varies across species.

Transgenic overexpression of neuromedin U promotes leanness and hypophagia in mice.

Recent work has shown that neuromedin U (NmU), a peptide initially identified as a smooth muscle contractor, may play a role in regulating food intake and energy homeostasis. To further evaluate this putative function, we measured food intake, body weight, energy expenditure and glucose homeostasis in transgenic mice that ubiquitously overexpress murine proNmU. NmU transgenic mice were lighter and had less somatic and liver fat, were hypophagic, and had improved insulin sensitivity as judged by an intraperitoneal insulin tolerance test. Transgenic mice had higher levels of hypothalamic NPY, POMC and MCH mRNA. There was no difference in O2 consumption between genotypes; however, NmU transgenic mice displayed a modest increase in respiratory quotient during food deprivation and refeeding. There were no behavioral disturbances in the NmU transgenic mice that could account for the results (e.g. changes in locomotor activity). When placed on a high-fat diet, transgenic mice remained lighter than wild-type mice and ate less, but gained weight at a rate similar to wild-type mice. Despite the increased weight gain with high-fat feeding, glucose tolerance was significantly improved in the transgenic mice. These findings support the hypothesized role of NmU as an endogenous anorexigenic peptide.

[Child labour]. / Il lavoro minorile.

The authors emphasize the violation of children's and adolescents' rights as a result of the exploitation of child labour. Besides the legal aspect, they pointed out the medical features related to the delicate growing process of the child in the phases of development and adaptation of the main organs to hard work. Currently the problem is being supervised by those states that recognize the right for minors to be protected against any kind of physical, mental, spiritual and moral risk.

[Pregnancy and work]. / Gravidanza e lavoro.

In the last decades there has been an increase of women's presence in the working field. That has been a consequence of the use of an advanced technology, of a different organization of work, of the rules about men/women equality, as wellas of the women's emancipation. As a result women have been exposed to a greater amount of risks both for their health and for their reproductive function. In this work, we highlighted the pathogenic effects resulting from the interaction of different substances present in the working place with the female organism, with special interest in the effects on pregnancy. Furthermore, we laid stress on the rules and on the guidelines that safeguard women's right to work as well as their right to be mothers both at a national and at an european level.

[The Pharaohs: slave merchants or organizers of an efficient welfare system? Re-writing history through new documentation and research]. / I Faraoni: schiavisti o inventori di una innovativa organizzazione del lavoro? Riscrittura della storia con l'ausilio di nuovi documenti, studi e interpretazioni.

The Authors have studied documents dating from the era of the Pharaohs, that is to say: the papyruses of Ebers and Smith, and have come to the conclusion that the recent claims by some archaeologists that the Egyptian workmen who built the Pyramids, were protected by health regulations and not treated as slaves, is true. This is a new interpretation of Egyptian history, because up to now, it has always been thought that the workmen were slaves. The construction of the Pharaoh's tomb was a team work including members of the upper classes (architects, physicians) and the lower classes (masons, workmen) and was the demonstration of a contribution and a symbol of recognition from all members of the team to the head of the society (the Pharaoh), who was their employer and took their interests into consideration e.g. health regulations. The Authors have also deduced from other sources of information that a public health system and welfare organization, comparable to our present day Legislative Decree 626/94, did in fact exist in the ancient world.

[Within the foundation of the Rome Institute of Experimental Hygiene in the year 1885: the climate of Rome]. / In margine all'inaugurazione dell'Istituto d'Igiene Sperimentale di Roma nel 1885: il clima di Roma.

To celebrate Prof.rs Vittorio Del Vecchio and Salvatore Ugo D'Arca, respectively the sixth and the seventh director of the Institute of Hygiene of the University of Rome, the Authors present and discuss the conferences on The climate of Rome given by Prof. Corrado Tommasi-Crudeli, founder and first director of such an Institute back in 1885. Particularly, the Authors illustrate the six tables shown by the speaker, which represent the scientific basis for the reclamation of Roman "Agro", affected by malaria at that time.

Osteotomía acetabular por doble abordaje en decúbito lateral: bases anatómicas y técnica quirúrgica / Dual osteotomy of the acetabulum: anatomic bases of a new technique

Las osteotomías periacetabulares de reorientación del cótilo tienen una reputación de ser difíciles desde el punto de vista técnico. Los autores presentan una técnica de osteotomías juxta acetabular que respeta la columna posterior del acetábulo, al igual que la técnica de GANZ. La osteotomía es de realización más simple, gracias a una instalación que permite realizar dos abordajes simultáneos. Los trazos de osteotomías son rectilíneos y controlables en permanencia, a través del intensificador de imágenes, y no constituyen ningún riesgo para el aporte vascular esencial de la arteria obturatriz y sus ramas. Su otro interés viene representado por la posibilidad de corregir fácilmente la retroversión parasitaria asociada al descenso de todo el cótilo. Esto permite evitar las malas posiciones que afectan el centro de la articulación y las amplitudes acetabulares

[Circadian variations in the onset of acute myocardial infarction]. / Variazione circadiana dell'insorgenza dell'infarto miocardico acuto.

BACKGROUND: Several studies have observed a circadian pattern in the onset of acute myocardial infarction (AMI), with a peak incidence in the morning hours. It has been suggested that different circadian rhythms may exist in various subgroups of patients. METHODS: This study sought to determine whether the circadian incidence of AMI varied by sex, age, cardiovascular risk factors, previous history of ischemic accidents, the site of AMI, and the short-term outcome. These possibilities were examined in a population of 597 consecutive patients with AMI, admitted to the coronary care unit. 548 patients have been included in the study, 442 men (80.6%) and 106 women (19.4 %); mean age 64.5 years. RESULTS: A peak incidence of AMI was found between 06.01 a.m. and 12.00 a.m. (32.4%; p<0.0002). This peak was present in patients 65 years old (33.2%; p<0.005), in men (32.5%; p<0.0002) but not in women, in smokers (32.1%; p<0.0005) and in those that did not smoke (33.0%; p<0.04), in patients with hypercholesterolemia (34.9%; p<0.006 ) and without hypercholesterolemia (31.1%; p<0.03). A circadian rhythm was absent in diabetics, hypertensives and in patients with a history of previous cardiovascular events. Regarding the site of AMI, inferior AMI showed an increased incidence between 06.01 a.m. and 12.00 a.m. (36.2%; p<0.002), while the circadian distribution of anterior AMI, as well as non-Q wave AMI, did not show this incidence. Finally, higher mortality was reported in patients with an AMI onset at night (22.3%). CONCLUSIONS: These results give further clues in understanding the external and inner factors acting in the morning hours as triggers for AMI.

Revisión y simplifición del abordaje anterior mínimo de la columna lumbar: fundamentos anatómicos del abordaje y presentación de una serie de casos / Revision and simplification of the anterior lumbar surgery: anatomic basis of minimal approach to lumbar spine

En este estudio se evaluaron los pacientes que requirieron amputaciones por Este artículo describe el abordaje retroperitoneal anterior mínimo, el cual proporciona acceso a cualquier nivel discal y vertebral entre T12 y S1. Se realiza una técnica de disección retroperitoneal que facilita la movilización renal y duodeno-pancreática para acceder a la cara anterior izquierda de toda la columna lumbar y de la unión tóraco-lumbar. Se realizaron disecciones anatómicas cuidadosas en cadáveres frescos y preservados para determinar la topografía y las relaciones anatómicas de interés y así desarrollar un abordaje seguro y fácilmente reproducible. Se ha prestado especial atención a la descripción de las venas lumbares y las anastomosis entre la vena renal izquierda y el sistema hemiácigos para la exposición de la cara anterolateral izquierda de T12 y L1. Se reporta una serie de 94 pacientes con lesiones ocasionadas por traumas o procesos degenerativos. Para todos los niveles lumbares, incluso en pacientes con antecedente de cirugía intraperitoneal, el abordaje retroperitoneal mínimo resultó ser seguro para los riñones, uréteres, bazo, plexo hipogástrico y unión duodeno-pancreática. Se reportan mejores resultados cosméticos, disminución del tiempo quirúrgico, escaso sangrado intraoperatorio y facilidad para la decorticación y colocación de injertos. El abordaje anterior retroperitoneal mínimo de la columna, desarrollado a partir de los abordajes retroperitoneales clásicos, ofrece ventajas significativas sobre las técnicas endoscópicas, las cuales requieren equipos sofisticados y son técnicamente demandantes. La exposición de todos los niveles lumbares, así como las maniobras de reducción y colocación de injertos, pueden realizarse con facilidad, sin ocasionar daño muscular

Effect of transient early hyperthyroidism on onset of puberty in Suffolk ram lambs.

The thyroid function and sexual development of eight 6-week-old Suffolk ram lambs were studied. The lambs were divided into either control or treatment groups and housed indoors. From 6 to 12 weeks of age, four lambs in the treatment group received 15 mg kg(-1) body weight per day of 6-propyl 2-thiouracil orally to suppress normal thyroid function. During the same period, thyroxine and tri-iodothyronine were injected s.c. at the rate of 8 and 16 microg kg(-1) body weight per day, respectively, to induce a hyperthyroid state. Four control lambs received sham injection and oral excipient. Concentrations of thyroxine, tri-iodothyronine, FSH, testosterone and insulin-like growth factor I were determined in blood collected by indwelling jugular catheters once a week, every 20 min from 09:00 to 15:20 h. Scrotal circumference was recorded each week. Semen collection was attempted by electro-ejaculation between weeks 17 and 36. Lambs were castrated at week 36 and testicular histology was examined. During the treatment period only, the concentration of thyroid hormones was higher in treated lambs than in controls (P < 0.05). From week 6 to week 9 only, concentrations of FSH in treated lambs were lower than in controls (P < 0.05). Insulin-like growth factor I concentrations were lower in treated lambs than in controls from week 10 to week 13 (P < 0.05). Frequency of testosterone pulses was higher (P < 0.01) in the treated lambs but concentrations were similar in the control and treated lambs throughout the experiment. Scrotal circumference was greater in treated lambs from week 26 to week 36 (P < 0.05). Treated lambs produced viable spermatozoa earlier than did control lambs. At week 36, sperm concentration in treated lambs was higher than in controls (P < 0.01) but semen volumes were similar (P > 0.1). Diameter of the seminiferous tubules in treated lambs was larger than in controls (P < 0.05). In conclusion, transient neonatal hyperthyroidism decreased FSH and insulin-like growth factor I concentrations temporarily, increased testosterone pulses and sperm production and advanced puberty in Suffolk ram lambs.

[Corrado Tommasi-Crudeli, hygienist]. / Corrado Tommasi-Crudeli, Igienista.

To celebrate the centennary of the death of Prof. Corrado Tommasi-Crudeli, the founder and first director of the "Istituto di Igiene Sperimentale" in Rome (1885), the authors remember this eminent scientist who spent his life not only working in scientific experimental research but also was a member of the Parliament and promotor of important laws regarding social and health problems. It should be remembered that, prior to 1870, Hygiene was not a discipline in itself, but was part of Medicine and taught in theoretical courses such as Public Health Inspection and Legal Medicine. On the basis of the discoveries by Pettenkofer in Munich (1883) and Koch in Berlin (1885), the Prof. Tommasi-Crudeli created the foundations of Hygiene in Rome as an independent subject, strictly linked to experiments thus realizing a theoretic/practical discipline. From that time on words, Hygiene has evolved and grown, to become a specialised subject whose objectives are epidemiological studies, prevention of diseases and promotion of health, and whose important contributions to other branches such as Microbiology, Parasitology and Medical Statistics have helped them to become independent disciplines.

Receptor-type protein-tyrosine phosphatase mu is expressed in specific vascular endothelial beds in vivo.

We investigated the localization of receptor-type protein-tyrosine phosphatase mu (RPTPmu) in tissues by immunofluorescence. RPTPmu immunoreactivity was found almost exclusively within vascular endothelial cells. RPTPmu was more abundant in the arterial tree than in the venous circulation. This pattern of expression was opposite to that of the von Willebrand factor and demonstrated a lack of difference in expression of VE-cadherin. RPTPmu was undetectable in the endocardium. In agreement with previous work on nonendothelial cell lines, RPTPmu was exclusively at the lateral aspects of endothelial cells in vivo and at cell-cell contacts as well as ex vivo in two- or three-dimensional endothelial cell cultures, and expression levels were upregulated by cell density. RPTPmu was detected in few other cells: bronchial and biliary epithelia and cardiocytes (intercalated discs). Our results identify RPTPmu as a new marker of endothelial cell heterogeneity and suggest a possible role in endothelial-specific functions, involving cell-cell contact.