Involvement of adrenal medulla grafts in the open field behavior.

Immunohistochemical and behavioral techniques were used to study the effects of adrenal medulla grafts, implanted in striatum after bilateral kainic acid (KA) lesions of this structure, on the open field behavior of mice. KA-induced behavioral changes in leaning, grooming and locomotor activity of the open field test were significantly improved after grafting of the adrenal medulla, and in some respects, fully restored. Immunohistochemical identification showed that grafts contained neuron-like cells with a tyrosine hydroxylase (TH), phenylethanolamine N-methyltransferase, gamma-aminobutyric acid (GABA), choline acetyltransferase (ChAT), and enkephalin-like immunostainings. A likely interpretation of this complex pattern of results is that adrenal medullary grafts may restore the deficits of GABAergic neurons which in turn reverse the abnormalities in emotionality and locomotion. Neurobiologically, these behavioral improvements probably involve GABAergic and catecholaminergic factors of adrenal medulla grafts, although other neuroactive substances, such as acetylcholine and enkephalins, cannot be excluded.

[Right subclavian catheterization. Misplaced insertion into the subclavian artery and the ascending aorta]. / Cathétérisme sous-clavier droit. Fausse-route dans l'artère sous-clavière et l'aorte ascendante.

The accidental subclavian artery puncture is usually obvious. We report a case of unrecognized arterial catheterisation. The catheter had been inserted during anaesthesia after return of dark and non pulsatile blood, and not controlled by a chest radiograph. During surgery, the injection of 40 mL isotonic saline containing 4 g of piperacillin for antibiotic prophylaxis resulted in a transient circulatory collapse associated with ECG tracing of myocardial ischaemia. Postoperative chest radiograph showed that the catheter was in a midsternal position, at the level of the ascending aorta. The intracoronary penetration of piperacillin was considered as the cause for the transient cardiocirculatory changes. The various diagnostic tools of the intra-arterial location of the catheter are discussed. All inadvertent subclavian artery catheterisations published in the literature have been carried out with multi-lumen catheters. The latter can contribute to the failure to recognize the arterial puncture and catheter insertion because of the use of a small bore needle (Seldinger's technique) and infusion with electrical pumps.

Neurobiology of consciousness: an overview.

The aim of this review is to connect the phenomenology of consciousness to its neurobiology. A survey of the recent literature revealed the following points. (1) Comprehensive descriptions of consciousness, of its subjective as well as of its objective aspects, are both possible and necessary for its scientific study. An intentionality-modeling structure (an unified and stable ego refers to objects or to itself in the framework of a stable, reproducible, predictable world) accounts for the main features. (2) The material basis of consciousness can be clarified without recourse to new properties of matter or to quantum physics. Current neurobiology appears to be able to handle the problem. In fact, the neurobiology of consciousness is already in progress, and has achieved substantial results. At the system level, its main sources of data are: the neurophysiology of sleep-wakefulness, brain imaging of mental representations, attention and working memory, the neuropsychology of frontal syndrome, and awareness-unawareness dissociations in global amnesia and different forms of agnosia. At an intermediate level of organization, the mechanisms of consciousness may be the formation of a certain kind of neural assembly. (3) Further research may focus on neuropsychology and neurophysiology of object perception and recognition as a natural model of intentionality, perception of time, body schema, interhemispheric communications, 'voluntary' acts and mental images. The synthetic and dynamic views provided by brain imaging may be decisive for discovering the neural correlates of the integrative aspects of consciousness. (4) The neurobiological approach may, beyond the finding of cellular and molecular mechanisms, improve the general concepts of consciousness, overcome their antinomies and, against epiphenomenalism, definitely establish the reality of consciousness.

A 'Neural Sampling Theory (NST)' of learning and memory mechanisms.

The purpose of the Neural Sampling Theory (NST) is to propose a plausible neurobiological explanation for some general properties of learning and memory (LM) phenomena, based on the parallelism and redundancy of the nervous system organization; on the psychological side, the NST is inspired by the Stimulus Sampling and Encoding Variability theories. The sampling process which is its core, is not purely random; it depends on temporal and intensity factors. The NST may be implemented at different levels of the nervous system: synapse, neuron, assembly of neurons. Moreover, it may be incorporated in other formal models and improve their degree of neural realism. For instance it allows to give a more realistic representation of the connection weight in the connectionist models and of the noisy character of the nervous system.

An introduction to the biology of consciousness.

This paper summarizes the main steps in a scientific study of consciousness. From a survey of the recent literature, it appears that: (1) there is a clear tendency to consider consciousness as a scientific object; (2) consistent subjective and objective descriptions of consciousness are possible; an intentional-modeling structure accounts for its main features; (3) from the evolutionary biology standpoint, conscious cognitive activities, as based on models of the self, the world and the alter-ego, have a functional value; (4) the material basis of consciousness can be clarified without recourse to new properties of the matter or to quantum physics. Current neurobiology, based on classical macrophysics, appears able to handle the problem. In this scope, the neurobiology of sleep-wakefulness and attention, and neuropsychology, have already achieved substantial advances.

Cholinergic differentiation factor/leukemia inhibitory factor enhances functional effects of adrenal medulla grafts after hippocampal lesions in rats.

We recently found that adrenal medulla grafts implanted into the hippocampus of rats survived for several months and significantly decreased the deficits produced by hippocampal lesions in the radial maze test [Jousselin-Hossaja et al. (1994) Neuroscience 59, 275-284]. These grafts contained choline acetyltransferase immunopositive chromaffin cells and received cholinergic innervation. In the experiments reported here, adrenal medulla grafts implanted in lesioned hippocampus were treated with cholinergic differentiation factor/leukemia inhibitory factor. In the presence of this factor, the number of chromaffin cells with cholinergic phenotypes increased as well as the beneficial effects of the grafts on the performances of rats in the radial maze. These results suggest that the functional effects of adrenal medulla grafted into the hippocampus set into play cholinergic mechanisms. The cholinergic differentiation factor/leukemia inhibitory factor may also have facilitated the survival and recovery of cholinergic neurons in the host tissue. However, due to the large range of action of this cytokine and the richness of the adrenal medulla contents, non cholinergic factors are also probably involved. Our results may help to elucidate the functions of the cholinergic differentiation factor/leukemia inhibitory factor since they provide the first indication that its intracerebral injection may have behavioral effects. Moreover, our data confirm the possibility of improving the efficiency of adrenal medulla implants in the central nervous system by appropriate treatments, not only by facilitating survival but also by selectively amplifying some potential factors of the graft. This might greatly enlarge the field of this grafting technique for analysing the normal functioning of the brain and for repairing it.

Effects of adrenal medulla grafts on memory capacities of rats after hippocampal lesions.

Behavioral and immunocytochemical techniques were used to study the effects of adrenal medulla grafts implanted in hippocampus--after lesion of this structure--on the memory capacities of rats. Performances of the grafted rats in the radial maze test were significantly improved and, in some aspects, fully restored. On the other hand, grafts had no significant effects on a one-trial spatial recognition test and impaired object recognition. Immunocytochemical identification showed that the grafts contained chromaffin cells with a choline acetyltransferase stainings while, in parallel, phenylethanolamine-N-methyltransferase stainings seemed to be decreased. Cholinergic innervation was established between the graft and the host hippocampus. A likely interpretation of this complex pattern of results is that the functional effects of the grafts depended on the arousal level induced by the behavioral task. At the neurobiological level, these effects probably set into play an interaction between opioid, catecholaminergic and cholinergic factors. Our results may contribute to the clarification of the problem of specificity of functional effects of intracerebral grafts as well as the problem of hippocampal role in learning and memory.

Relationships between arousal and cognition-enhancing effects of oxiracetam.

Cognition-enhancing effects of no-otropic drugs are currently ascribed to an increase in arousal level. In order to test this hypothesis, we studied the effects of three doses of oxiracetam (25, 50 and 100 mg/kg IP) on a radial maze task and on slow wave sleep (SWS) latency in a familiar environment. The 25- and 100-mg/kg doses, but not the 50-mg/kg, significantly improved performance in the memory task. On the other hand, SWS latency was significantly increased by 50 and 100 mg/kg, with the effect of the 25-mg/kg dose going in the same direction but only approaching significance. These results give only a partial support to the "arousal factor hypothesis." Other factors are probably involved in the promnesic effects of oxiracetam.

Spatial but not object recognition is impaired by aging in rats.

Four groups of male Wistar rats (4, 8, 18, and 24 months) were submitted to object recognition and spatial recognition tests. Object recognition was not significantly affected by aging even at a longer retention interval. On the contrary, spatial recognition was significantly impaired in 18- and 24-month groups. The existence of two dissociable neural systems, respectively involved in object and spatial recognition, is discussed.

The Roman strains of rats as a psychogenetic tool for pharmacological investigation of working memory: example with RU 41656.

This study examined the effects of RU 41656, a dopaminergic D2 agonist, on the differential working memory performances and on the differential activities of the neurochemical systems of the Roman high (RHA) and Roman low (RLA) avoidance strains of rats. Compared with RLA, RHA performed worse in three tests of working memory (spontaneous alternation, radial maze and object recognition) and had higher levels of exploratory locomotor activity. Hippocampal and frontal cortex choline acetyltransferase (ChAT) activities were lower in RHA. Frontal cortex DA and DOPAC levels, hippocampal and striatal 5-HT and NA levels were higher in RHA. RU 41656 induced a significant improvement in working memory performance of RHA, whereas in RLA it had no effect. It decreased exploratory locomotor activity in both strains. ChAT activity in hippocampus was not affected by RU 41656 in either strain, whereas in frontal cortex it was increased in RHA but not in RLA. Hippocampal NA levels were decreased by RU 41656 in RHA but not in RLA. These results confirm previous data concerning the promnesic effect of RU 41656 and extend the finding that the Roman strains are a psychogenetic model for the behavioural, neurochemical and psychopharmacological study of the working memory in rats.

Selective fimbria and thalamic lesions differentially impair forms of working memory in rats.

Several series of experiments were designed to compare the effects of selective lesions of the fimbria or of thalamic nuclei on three different tasks involving working memory in rats: object recognition, place recognition, and the radial arm maze test. The main effects of fimbria lesions were as follows: they produced deficits in the radial maze; object recognition was spared or even facilitated, whereas place recognition was impaired. Electrolytic lesions of either centromedian-parafascicularis (CM-Pf) or dorsomedialis (DM) nuclei produced highly significant deficits in the radial maze test but spared object and place recognition. Ibotenate lesions of the CM-Pf had no effect on any test, which means that the critical structure in the effects of the electrolytic lesions of the CM-Pf was the fasciculus retroflexus (FR). These data may contribute two main points to animal models of hippocampal and thalamic amnesia: (1) different forms of working memory in rats might have different neural bases and (2) the FR may be involved in learning and memory processes.