Rapid diagnosis of cytomegalovirus by indirect immunofluorescence assay with monoclonal antibody F6b in a commercially available kit.

Anti-human cytomegalovirus (CMV) monoclonal antibody (MAb) F6b produced by clone 95/12 was used for the rapid diagnosis (16 to 24 h) of CMV isolates in 308 clinical specimens and compared with classic isolation. MAb F6b gave 100% correlation with isolation. When this MAb was used for direct diagnosis with 212 urine and bronchoalveolar specimens, detection of CMV varied from 50 to 75% compared with viral isolation.

[Value of rapid diagnosis of Plasmodium falciparum using indirect monoclonal immunofluorescence]. / Intêret du diagnostic rapide de Plasmodium falciparum par immunofluorescence indirecte monoclonale.

Development and use of murine specific monoclonal antibodies associated with an indirect immunofluorescent assay allows a rapid detection of P. falciparum antigens. This work compares the results obtained by interpretation of Giemsa staining technique on blood smears, to the use of a commercial reagent (Monofluo Kit P. falciparum). This monoclonal indirect immunofluorescent assay (I.F.I.M.) provides the clinician with a precious aid to diagnosis as well by its specificity and sensitivity, better than conventional staining techniques; particularly in pauciparasitaemia induced by prime-infestation or partial anti-malarial drug resistance.