RESUMO
Sucrose, nycodenz, metrizamide and a mixture of equal volumes of sucrose and metrizamide were used as density gradient media for the isolation of retinal rod outer segments. The high osmolarity of sucrose had a strongly negative effect on the nature of the rod outer segments, whereas they were much better preserved using iodinated density gradient media such as nycodenz and metrizamide for their isolation.
Assuntos
Células Fotorreceptoras , Segmento Externo da Célula Bastonete , Animais , Bovinos , Fracionamento Celular/métodos , Centrifugação com Gradiente de Concentração/métodos , Iodo , Microscopia EletrônicaRESUMO
Glucosamine can inhibit the development of viral cytopathogenic effect and the production of infective viral particles of both enveloped and non-enveloped viruses. The extent of antiviral activity is dependent on drug concentration, composition of the culture medium and type of cell host.
Assuntos
Glucose/farmacologia , Vírus/crescimento & desenvolvimento , Animais , Embrião de Galinha , Replicação do DNA/efeitos dos fármacos , Especificidade da Espécie , Células Vero , Replicação Viral/efeitos dos fármacos , Vírus/efeitos dos fármacosRESUMO
Angiotensin I converting enzyme (ACE) was studied in Vero cells, rabbit corneal fibroblasts, and rabbit corneal endothelial cells. The enzyme activity was determined by means of an assay employing hippuryl-glycyl-glycine as a substrate. The hippuric acid end product was separated from the substrate by reversed phase liquid chromatography and measured spectrophotometrically at 228 nm. The enzyme was further characterized by a captopril inhibition study. Significant ACE activity was found in rabbit corneal endothelial cells but not in other types of cells tested. This is the first report of the presence of this enzyme in a specific ocular cell type and suggests that angiotensin II may play a role in normal ocular physiology.
