RESUMO
In Puerto Rico, an island threatened by climate warming, only one of two species of frogs that share part of their distribution has undergone a recent range contraction to higher elevations. We questioned if differences in their physiological response to temperature and dehydration might explain this distributional change. We studied a lowland and a highland population of Eleutherodactylus coqui, a widespread generalist, and E. portoricensis, an endangered species that is currently found only above 600 m. We compared various physiological aspects: operative temperature; temperature selection; critical temperatures; and their response to jumping performance tests at various thermal and hydric regimes. Results revealed that E. portoricensis had the highest CTmin and lowest CTmax and selected a cooler range of temperatures from the experimental gradient. Jumping performance increased with temperature for the three populations until attaining maximum performance. Afterwards, performance dropped drastically until reaching CTmax. Dehydration had a negative effect on performance for both species, particularly on maximum performance. This effect was greatest for E. portoricensis, followed by high-elevation E. coqui. The significantly greater thermo-hydric physiological limitations of E. portoricensis may explain its recent range contraction, potentially, as a response to climate warming. Low-elevation E. coqui had the lowest operative warming tolerance and was the only population to select temperatures like those encountered in their environment, indicating it may be narrowly adapted to local thermal conditions and thus, also vulnerable to climate change. Our results point towards plasticity in the response of E. coqui to varying climatic conditions, and present evidence of different physiological responses between closely related species at the same locality. This work highlights the importance of studying the combined effects of temperature and hydration to understand the response of ectotherms to warming environments and presents further evidence that desiccation may be a limiting factor determining which species may survive.
