RESUMO
The expression of lactoferrin (LF), ceruloplasmin (CP) and secretory component (SC) in various thyroid diseases was investigated using an immunohistochemical method. LF is an iron-binding protein overexpressed in certain human adenocarcinomas. CP is implicated in carcinogenesis and SC is part of the local immune defense system. Sections of 15 follicular adenomas, 5 follicular carcinomas (FC), 16 papillary carcinomas (PC), 15 goitres and 2 cases of lymphocytic thyroiditis were re-examined and investigated immunohistologically by a streptavidin-biotin method. A positive immunostain reaction for CP and LF was observed in all cases of PC and FC. A positive immunostain reaction for SC was observed in 13/16 cases of PC and 4/5 cases of FC. A negative immunostain reaction for CP, LF and SC was observed in medullary cancers, in follicular adenomas and in the cases with goitre. We conclude that the immunostain expression of LF, CP and SC is a valuable diagnostic aid in the differential diagnosis between benign and malignant thyroid tumors. The expression of SC indicates the presence of a local immunodefensive system.
Assuntos
Adenocarcinoma Folicular/enzimologia , Adenoma/enzimologia , Carcinoma Papilar/enzimologia , Ceruloplasmina/análise , Lactoferrina/análise , Componente Secretório/análise , Doenças da Glândula Tireoide/enzimologia , Neoplasias da Glândula Tireoide/enzimologia , Adenocarcinoma Folicular/patologia , Adenoma/patologia , Carcinoma Papilar/patologia , Diagnóstico Diferencial , Regulação Neoplásica da Expressão Gênica , Humanos , Imuno-Histoquímica , Doenças da Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/patologiaRESUMO
UNLABELLED: The aim of this study was to investigate the presence of a local immunological defense mechanism of the secretory IgA class in the female genital tract. MATERIAL AND METHODS: We studied by a streptavidin-biotin method the secretory component (SC) and IgA distribution in paraffin-embedded sections of 90 formalin-fixed specimens. We studied 10 normal and 5 neoplastic cervical specimens, 20 normal, 10 hyperplastic endometrial specimens and 10 endometrial adenocarcinomas, 5 normal ovarian tubes and 30 ovarian epithelial neoplasms, serous and mucinous. A polyclonal SC and (Dako) and a mab IgA (Dako) was used and the reaction was scored from 1-3. RESULTS: Normal cervical mucosa and atrophic endometria were negative, while the basal portion of the endometrium, focally the proliferative glands, most of the secretory glands and most of the hyperplastic glands were positive for SC. IgA showed a similar distribution and a perivascular stromal reaction. Adenocarcinomas were positive for SC, but the intensity of the reaction was dependent on the differentiation of the tumors. Mucous and most serous neoplasms were negative for SC. IgA showed a similar reaction. CONCLUSION: There is evidence that the female genital tract has a local defensive immune system that may be hormone dependent. SC is a valuable marker of glandular differentiation.
Assuntos
Neoplasias dos Genitais Femininos/imunologia , Genitália Feminina/imunologia , Imunoglobulina A Secretora/metabolismo , Componente Secretório/metabolismo , Adenocarcinoma/imunologia , Estudos de Casos e Controles , Colo do Útero/imunologia , Cistadenocarcinoma Seroso/imunologia , Cistadenoma Seroso/imunologia , Neoplasias do Endométrio/imunologia , Tubas Uterinas/imunologia , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Ovarianas/imunologia , Ovário/imunologia , Neoplasias do Colo do Útero/imunologiaRESUMO
The development of stromal sarcomas on the foci of endometriosis is extremely rare and the differential diagnosis from other tumors of myogenic, vascular, hemopoietic or epithelial origin may present great diagnostic difficulties. We investigated the clinicopathological and immunohistochemical characteristics of 4 cases of endometrial stromal sarcoma that developed on endometriotic foci of the uterus, vagina and omentum. Thye were classed as high grade (1/4) or low grade (3/4) malignant potential tumors, according to their mitotic activity. Immunohistochemically these tumors gave a positive reaction to vimentin, but were negative to desmin, smooth muscle actin, factor VIII, EMA and LCA. These characteristics permit their identification and a proper therapeutic approach.
Assuntos
Neoplasias do Endométrio/patologia , Endometriose/patologia , Omento/patologia , Neoplasias Peritoneais/patologia , Sarcoma do Estroma Endometrial/patologia , Neoplasias Uterinas/patologia , Neoplasias Vaginais/patologia , Biópsia por Agulha , Diagnóstico Diferencial , Intervalo Livre de Doença , Neoplasias do Endométrio/complicações , Neoplasias do Endométrio/cirurgia , Endometriose/complicações , Feminino , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Omento/cirurgia , Neoplasias Peritoneais/complicações , Neoplasias Peritoneais/cirurgia , Prognóstico , Sarcoma do Estroma Endometrial/complicações , Sarcoma do Estroma Endometrial/cirurgia , Resultado do Tratamento , Neoplasias Uterinas/complicações , Neoplasias Uterinas/cirurgia , Neoplasias Vaginais/complicações , Neoplasias Vaginais/cirurgiaRESUMO
We report an unusual case of mandibular sarcoma which developed in the field of irradiation of a patient treated successfully 10 years previously for nasopharyngeal carcinoma (NC). Although second malignant neoplasms are not rare among survivors of childhood cancer, this event is not included among the type of failures and complications of treatment of NC occurring either in children.
