Clinical Study Regarding Arrhythmogenic Risk Factors and Oxidative Stress Inductibility in Young People.

Rhythm disorders in young people are often reported and when they are persistent, repetitive or with a severity degree, an ethiopathgenical assessment of arrhythmogenic risk factors and their implications is required. PURPOSE: Arrhythmogenic risk factors evaluation and the possibility of determining oxidative stress in the arrhythmic pathology in young people. MATERIAL AND METHODS: The study was conducted on 184 young subjects, aged 16-26 years old; the points of interest were: the presence or absence of cardiac dysrhythmias, the existence of proarrhythmogenic risk factors and determination of oxidative stress status modifications. RESULTS: Of the studied young subjects, 39% presented heart rhythm disturbances, repetitive or persistent (atrial extrasystolic arrhythmia, sinus tachycardia, ventricular extrasystolic arrhythmia, paroxysmal atrial fibrillation, paroxysmal supraventricular tachycardia, associated dysrhythmias, atrial flutter, sinus bradycardia), which have been associated with the following risk factors: coffee consumption 82%, stress 80%, physical effort 72%, energy drinks consumption 72%, hyperlipidic diet 69%, familial predisposition 69%, alcohol intake 53%, frequent sleep deprivation 50%, smoking 31%, overweight 31%. The observed risk factors may be involved in the increasing of oxidative stress level, and, for this reason, the determination of oxidative stress biomarkers is required. The association of arrhythmogenic risk factors, with the expression of oxidative stress markers and the existence of enzymatic genetic polymorphism of redox systems, requires proper monitoring for the further risk of endothelial lesions induction, leading to aterosclerosis. CONCLUSIONS: Arrhythmogenic risk factors and biomarkers of oxidative stress are important, especially in young people cases, for monitoring the cardiovascular risk, for primary prevention and early treatment.

[Effect of some biotic elicitors on flavonoids production in Digitalis lanata cell cultures]. / Efectul unor elicitori biotici asupra productiei de flavone in culturi celulare de Digitalis lanata.

UNLABELLED: A method to improve the secondary metabolites production in plant cell cultures is the use of elicitors (biotic or abiotic). In this paper we investigated the effect of two biotic elicitors, Botrytis and Sclerotinia, on the flavonoids production, an important category of secondary metabolites, in Digitalis lanata suspension cultures. MATERIAL AND METHOD: Were used two cell lines: line 11 and line 13C 100 (aged 10 days). Were used two concentrations of elicitors (1 ml and 2 ml/100 ml suspension, respectively). Flavones were determined by a spectrophotometric method, according to Romanian Pharmacopoeia no. X (Cynarae Folium monography). RESULTS: For both cell lines and for both elicitors, flavonoids accumulation grew with elicitation time and elicitor concentration. The highest flavonoids production took place after 96 hours elicitation time for the highest elicitor .concentration (2ml/100ml suspension). So, line 11 accumulated 1000 mg% flavonoids with Botrytis and 999.81 mg% with Sclerotinia. Line 13C 100 accumulated 1051,65 mg% flavonoids with Botrytis and 1025,43 mg% with Sclerotinia. For all experiments, Botrytis (both concentration) induced a higher flavonoids accumulation for line 11 than Sclerotinia. Line 13C 100 responses to elicitor action is stronger for Sclerotinia for higher elicitor concentration than for same concentration of Botrytis.

Plant regrowth from potato shoot tips cryopreserved by a combined vitrification-droplet method.

Shoot tips obtained from in vitro potato plants (three cultivars) were successfully cryopreserved by the combined vitrification-droplet method and subsequently regenerated shoots. The effect of apex size, sucrose concentration, preculture duration and cold hardening treatments on viability of cryopreserved shoot tips was studied. The excised shoot tips were incubated, precultured and dehydrated with concentrated PVS2 cryoprotective solution at room temperature, prior to a direct plunge in liquid nitrogen. After rapid rewarming in Murashige-Skoog (MS) liquid medium, shoot tips were plated on semisolid MS medium (3.5 g/l agar) supplemented with 0.4 mg l(-1) gibberellic acid, 0.5 mg l(-1) zeatin, 0.2 mg l(-1) indole-3-acetic acid and 30 g l(-1) sucrose for regrowth. Cryopreserved shoot tips resumed growth within 20 days and regenerated shoots within 30 days. The highest regrowth levels of apices after cryopreservation were 55% recovery for cv. Désirée, 51% for cv. Ostara and 46% for cv. Santé.