RESUMO
Herbicides are the most widely used class of pesticides in the world. Their intensive use raises the question of their harmfulness to the environment and human health. These pollutants need to be detected at low concentrations, especially in water samples. Commonly accepted analytical techniques (HPLC-MS, GC-MS, ELISA tests) are available, but these highly sensitive and time-consuming techniques suffer from high cost and from the need for bulky equipment, user training and sample pre-treatment. Biosensors can be used as complementary early-warning systems that are less sensitive and less selective. On the other hand, they are rapid, inexpensive, easy-to-handle and allow direct detection of the sample, on-site, without any further step other than dilution. This review focuses on enzyme- and cell- (or subcellular elements) based biosensors. Different enzymes (such as tyrosinase or peroxidase) whose activity is inhibited by herbicides are presented. Photosynthetic cells such as algae or cyanobacteria are also reported, as well as subcellular elements (thylakoids, chloroplasts). Atrazine, diuron, 2,4-D and glyphosate appear as the most frequently detected herbicides, using amperometry or optical transduction (mainly based on chlorophyll fluorescence). The recent new WSSA/HRAC classification of herbicides is also included in the review.
