Down-regulation of CatSper 1 and CatSper 2 genes by lead and mercury.

In the study of the expression of CatSper genes, consideration of the effects of environmental metal toxicity is very important. Therefore, in this study, the effects of lead acetate and mercury chloride exposure on expression of CatSper genes, sperm parameters, histology of testis and prooxidant antioxidant balance (PAB) values of serum were investigated. A total of 28 mice was divided into four groups. The control group did not receive injections. The sham group received normal saline intraperitoneally. Lead and mercury groups were injected 60 and 1.25 mg/kg/daily lead acetate and mercury chloride respectively intraperitoneally for 2 weeks. After 35 days, the sperm analysis and histology of left testis were performed. In addition, serum was obtained to measure the PAB values. The right testis was used for molecular analysis of real-time PCR. Administration with either lead acetate or mercury caused significant damage to the seminiferous tubules as well as a reduction in sperm parameters compared to the control group. The relative expression of CatSper 1 and CatSper 2 in the lead group was lower than that of the control group (-0.01 ±â€¯0.24, -0.007 ±â€¯0.52 vs. 1 ±â€¯0.50, P = 0.34). The relative expression of CatSper 1 and CatSper 2 was significantly lower in the mercury group compared to the control ones (-0.24 ±â€¯2.28, -4.49 ±â€¯4.86 vs. 1 ±â€¯0.50, P = 0.21). PAB values significantly increased in lead or mercury exposed- mice compared to the control ones (0.93 ±â€¯0.17, 1.54 ±â€¯0.17 vs. 0.51 ±â€¯0.11; P ≤ 0.000). The results of this study showed that administration with either lead acetate or mercury chloride caused degenerative damage in seminiferous tubules and reduction in sperm quality and expression of CatSper 1, 2 genes in mice. Therefore, it is possible in infertile men who have had exposure to lead acetate or mercury chloride. Owing to structural similarities, these metals are substitutes for calcium ions and have effects on calcium channels. These cause immobility in sperm by blocking CatSper-specific calcium channels. However, more studies are required to elucidate the mechanism underlying the impact of different doses of heavy metals on CatSper genes expression.

Correlation between Apoptosis and Histological Grade of Transitional Cell Carcinoma of Urinary Bladder.

INTRODUCTION: To evaluate the relationship between histological grade and apoptotic index (AI) in transitional cell carcinoma (TCC) of urinary bladder. MATERIALS AND METHODS: Formalin-fixed and paraffin-embedded tissue blocks from 75 patients with TCC, who undergone transurethral resection (TUR) were studied. One 3-micron section was provided from each TUR samples. In one section after hematoxylin and eosin (H&E) staining, tumor grade was determined according to World Health Organization/International Society of Urology and Pathology (WHO/ISUP) criteria. The apoptotic cells were determined using a terminal deoxynucleotidyl transferase (TdT) mediated dUTP biotin nick end labeling (TUNEL) technique. Apoptotic index was then obtained as the percent of TUNEL positive cells from observations of at least 1000 cells in each section. RESULTS: Forty-nine patients were men and 26 were women. Mean age was 56.349 years. Mean AI was 2.300.50. The relationship between grade and AI was significant (p=0.000, r=0.551); a higher grade was associated with a higher AI. CONCLUSION: Apoptosis index has a positive correlation with bladder TCC's grade. Further studies are needed to better determine the effect of apoptosis index on prognosis.

Ultrastructure of apoptotic oligodendrocytes in the spinal cord of adult rat with long-standing axotomised sciatic nerve.

The apoptosis in the oligodendrocytes was evaluated in adult rat which were axotomised and sacrificed three months aftersurgery. The apoptotic cell had electron dense organelles with condensed cytoplasm, the nucleus showed chromatin condensation with formation of band-like structure of clumped chromatin, and subsequently the cell underwent progressive shrinkage. The advanced stage of apoptosis showed electron lucency in the chromatin band, followed by disintegration of the nucleus, while the cytoplasm was replaced with high electron dense granular substance forming round electron dense apoptotic cells. The autophagy was also noticed in the cytoplasm of these cells, consisting of membrane bounded cytoplasmic organelles. Because the autophagy was seen in the apoptotic cell, this indicates that the autophagy is not limited to autophagic type programmed cell death.