RESUMO
The enzyme immunoassay and neuraminidase activity inhibition test using polyclonal and monospecific antineuraminidase sera were employed to establish the similarities and differences in the antigenic structure of neuraminidase of influenza A viruses (H1N1), serovariant Hsw1N1, isolated from man in Alma-Ata (USSR), 1984, New Jersey (USA), 1976, and Pazardjik (BPR), 1982, as well as from swine and birds. Oligonucleotide mapping revealed significant structural differences in the genes coding for neuraminidase of Hsw1N1 viruses. The experimental results indicate a high degree of the enzyme variability in this group of viruses.
Assuntos
Antígenos Virais/análise , Epitopos/análise , Genes Virais , Vírus da Influenza A Subtipo H1N1 , Vírus da Influenza A/enzimologia , Neuraminidase/imunologia , Animais , Testes de Inibição da Hemaglutinação , Humanos , Vírus da Influenza A/genética , Vírus da Influenza A/imunologia , Neuraminidase/genética , Especificidade da EspécieRESUMO
The method of specific adsorption followed by the use of antisera in HI test and competitive enzyme immunoassay was used to study the antigenic composition of hemagglutinins (HA) Hsw1 in influenza viruses isolated in 1982 from humans in Bulgaria and in 1976 in Canada from ducks as well as their antigenic relationships with HA of Hsw1 variant isolated from swine and man. Hemagglutinins of Hsw1 strains isolated from man in Bulgaria and Alma-Ata were found to be similar to HA of A/New Jersey/8/76 virus in two determinants and with hemagglutinin of the classic virus of swine in three determinants. The HA of A/duck/Alberta/35/76 virus was similar in three determinants to HA of A/New Jersey/8/76 virus and in two determinants with other Hsw1 variants. The similarities and differences in antigenic determinants of HA in Hsw1 viruses isolated from man and animals attest to their common origin and different modes of variability.
Assuntos
Epitopos/análise , Hemaglutininas Virais/imunologia , Vírus da Influenza A Subtipo H1N1 , Vírus da Influenza A/imunologia , Animais , Patos , Ensaio de Imunoadsorção Enzimática , Humanos , Técnicas de ImunoadsorçãoRESUMO
The principal possibility of using antibodies conjugated with colloid gold for better visualization of viruses, viral antigens, and their immune complexes in electron microscope was studied on the experimental model of HBs-antigen and Venezuelan equine encephalomyelitis virus. The same method was used for the study of the thin structure of influenza A (H1N1) virus hemagglutinins using monoreceptor antisera.
Assuntos
Antígenos Virais/análise , Ouro , Animais , Complexo Antígeno-Anticorpo/análise , Reações Antígeno-Anticorpo , Coloides , Vírus da Encefalite Equina Venezuelana/imunologia , Antígenos de Superfície da Hepatite B/análise , Humanos , Imuno-Histoquímica , Vírus da Influenza A/imunologia , Camundongos , Microscopia Eletrônica , Proteínas Recombinantes/análiseRESUMO
Competitive radioimmunoassay was used to study the antigenic composition of hemagglutinin of Hsw1N1 viruses isolated from man in comparison with hemagglutinin Hsw1 of influenza virus of swine and ducks. The data of oligonucleotide analysis of the 4th RNA segment coding for hemagglutinin in these viruses are presented. It has been shown that in Alma-Ata, 1984-1985, influenza viruses Hsw1N1 were isolated with the antigenic structure of hemagglutinin and with the hemagglutinin gene identical with those of the classical influenza virus of swine A/Swine/Iowa/15/30 but differing from virus A/New Jersey/8/76.
Assuntos
Antígenos Virais/análise , Hemaglutininas Virais/análise , Vírus da Influenza A/imunologia , Adsorção , Animais , Antígenos Virais/genética , Ligação Competitiva , Patos , Genes Virais , Hemaglutininas Virais/genética , Humanos , Vírus da Influenza A/genética , Vírus da Influenza A/isolamento & purificação , Cazaquistão , Oligonucleotídeos/análise , Oligonucleotídeos/genética , RNA Viral/análise , RNA Viral/genética , Radioimunoensaio/métodos , SuínosRESUMO
The results of immunological analysis of the antigenic structure of hemagglutinin and neuraminidase of influenza A (serovariant Hsw 1) isolates recovered in Alma-Ata in 1984-1985 are presented. By means of specific adsorption and immunoprecipitation the antigenic structure of new isolates was shown to be very similar to that of the A/swine/Iowa/15/30 strain.
Assuntos
Vírus da Influenza A/imunologia , Influenza Humana/microbiologia , População Urbana , Anticorpos Antivirais/análise , Antígenos de Superfície/análise , Antígenos Virais/análise , Fenômenos Químicos , Físico-Química , Reações Cruzadas , Surtos de Doenças , Hemaglutininas Virais/análise , Humanos , Vírus da Influenza A/classificação , Vírus da Influenza A/isolamento & purificação , Influenza Humana/epidemiologia , Cazaquistão , Neuraminidase/análise , Inoculações Seriadas , SorotipagemRESUMO
The study made with the use of virological, seroepidemiological, radioimmunological and immunological techniques revealed that influenza virus A, antigenically similar to influenza virus A/swine/Iowa/15/30, circulated in Alma-Ata in 1984-1985. The role of these viruses in the seasonal rise of influenza morbidity at the end of 1984 was established. From nasal washings and blood clots obtained from patients, as well as from dissection material, 12 strains were isolated. These strains were similar to serovariant A/swine/owa/15/30 and differed from influenza virus A/New Jersey/76.
Assuntos
Variação Genética , Vírus da Influenza A Subtipo H1N1 , Vírus da Influenza A/isolamento & purificação , Influenza Humana/microbiologia , População Urbana , Anticorpos Antivirais/análise , Antígenos Virais/análise , Hemaglutininas Virais/análise , Humanos , Vírus da Influenza A/classificação , Vírus da Influenza A/imunologia , Influenza Humana/epidemiologia , Influenza Humana/imunologia , Cazaquistão , Estações do Ano , SorotipagemRESUMO
Isolated glycoproteins of influenza A/PR/8/34 (H1N1) administered intranasally to white mice were found to increase permeability of lung capillaries.