Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 13 de 13
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Parasitol Res ; 120(4): 1321-1333, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33164156

RESUMO

Schistosomiasis, caused by a blood fluke of the genus Schistosoma, afflicts over 230 million people worldwide. Treatment of the disease relies on just one drug, praziquantel. Cnicin (Cn) is the sesquiterpene lactone found in blessed thistle (Centaurea benedicta) that showed antiparasitic activities but has not been evaluated against Schistosoma. However, cnicin has poor water solubility, which may limit its antiparasitic activities. To overcome these restrictions, inclusion complexes with cyclodextrins may be used. In this work, we evaluated the in vitro and in vivo antischistosomal activities of cnicin and its complexes with ß-cyclodextrin (ßCD) and 2-hydroxypropyl-ß-cyclodextrin (HPßCD) against Schistosoma mansoni. Cnicin were isolated from C. benedicta by chromatographic fractionation. Complexes formed by cnicin and ßCD (Cn/ßCD), as well as by cnicin and HPßCD (Cn/HPßCD), were prepared by coprecipitation and characterized. In vitro schistosomicidal assays were used to evaluate the effects of cnicin and its complexes on adult schistosomes, while the in vivo antischistosomal assays were evaluated by oral and intraperitoneal routes. Results showed that cnicin caused mortality and tegumental alterations in adult schistosomes in vitro, also showing in vivo efficacy after intraperitoneal administration. The oral treatment with cnicin or Cn/ßCD showed no significant worm reductions in a mouse model of schistosomiasis. In contrast, Cn/HPßCD complex, when orally or intraperitoneally administered to S. mansoni-infected mice, decreased the total worm load, and markedly reduced the number of eggs, showing high in vivo antischistosomal effectiveness. Permeability studies, using Nile red, indicated that HPßCD complex may reach the tegument of adult schistosomes in vivo. These results demonstrated the antischistosomal potential of cnicin in preparations with HPßCD.


Assuntos
Schistosoma mansoni/efeitos dos fármacos , Esquistossomose mansoni/tratamento farmacológico , Esquistossomicidas/farmacologia , Sesquiterpenos/farmacologia , 2-Hidroxipropil-beta-Ciclodextrina , Administração Oral , Animais , Centaurea/química , Modelos Animais de Doenças , Composição de Medicamentos , Fezes/parasitologia , Feminino , Injeções Intraperitoneais , Masculino , Camundongos , Contagem de Ovos de Parasitas , Carga Parasitária , Permeabilidade , Praziquantel/farmacologia , Praziquantel/uso terapêutico , Esquistossomose mansoni/parasitologia , Esquistossomicidas/administração & dosagem , Esquistossomicidas/química , Esquistossomicidas/farmacocinética , Sesquiterpenos/administração & dosagem , Sesquiterpenos/química , Sesquiterpenos/farmacocinética , Solubilidade , beta-Ciclodextrinas
2.
J Agric Food Chem ; 66(28): 7275-7285, 2018 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-29925239

RESUMO

In the present work, hydrophobic nanoprecipitates (HNPs) of inclusion complexes formed between ß-cyclodextrin (ßCD) and the avermectins (AVMs) named eprinomectin (EPRI) and ivermectin (IVER) were synthesized and characterized, and their larvicidal activity against Aedes aegypti and human safety against fibroblasts were evaluated. Initially, thermogravimetric analysis/differential thermal analysis data revealed that inclusion increased the thermal stability of AVMs in the presence of ßCD. Nuclear magnetic resonance experiments and density functional theory calculations pointed out the inclusion of the benzofuran ring of the two AVMs in the ßCD cavity. Isothermal titration calorimetry experiments allowed identification of different binding constants for EPRI/ßCD ( Kb = 1060) and ßCD/IVER ( Kb = 1700) systems, despite the structural similarity. Dynamic light scattering titrations of AVMs' dimethyl sulfoxide solution in ßCD aqueous solution demonstrated that the formed HNPs have lower sizes in the presence of ßCD. Finally, the inclusion of EPRI in ßCD increased its larval toxicity and reduced its human cytotoxicity, while for IVER/ßCD no beneficial effect was observed upon inclusion. These results were rationalized in terms of structural differences between the two molecules. Finally, the EPRI/ßCD complex has great potential as an insecticide against A. aegypti larvae with high human safety.


Assuntos
Aedes/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Inseticidas/toxicidade , Ivermectina/análogos & derivados , Larva/efeitos dos fármacos , Nanoestruturas/toxicidade , beta-Ciclodextrinas/farmacologia , Aedes/crescimento & desenvolvimento , Animais , Sobrevivência Celular/efeitos dos fármacos , Feminino , Interações Hidrofóbicas e Hidrofílicas , Inseticidas/química , Ivermectina/química , Ivermectina/toxicidade , Larva/crescimento & desenvolvimento , Espectroscopia de Ressonância Magnética , Masculino , Nanoestruturas/química , Solubilidade , beta-Ciclodextrinas/química
3.
Drug Dev Ind Pharm ; 44(9): 1498-1505, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29683352

RESUMO

Losartan (Los), a non-peptidic orally active agent, reduces arterial pressure through specific and selective blockade of angiotensin II receptor AT1. However, this widely used AT1 antagonist presents low bioavailability and needs once or twice a day dosage. In order to improve its bioavailability, we used the host: guest strategy based on ß-cyclodextrin (ßCD). The results suggest that Los included in ßCD showed a typical pulsatile release pattern after oral administration to rats, with increasing the levels of plasma of Los. In addition, the inclusion compound presented oral efficacy for 72 h, in contrast to Los alone, which shows antagonist effect for only 6 h. In transgenic (mREN2)L27 rats, the Los/ßCD complex reduced blood pressure for about 6 d, whereas Los alone reduced blood pressure for only 2 d. More importantly, using this host: guest strategy, sustained release of Los for over a week via the oral route can be achieved without the need for encapsulation in a polymeric carrier. The proposed preformulation increased the efficacy reducing the dose or spacing between each dose intake.


Assuntos
Bloqueadores do Receptor Tipo 1 de Angiotensina II/química , Bloqueadores do Receptor Tipo 1 de Angiotensina II/farmacologia , Receptor Tipo 1 de Angiotensina/metabolismo , Administração Oral , Animais , Anti-Hipertensivos/química , Anti-Hipertensivos/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Química Farmacêutica/métodos , Portadores de Fármacos/química , Losartan , Masculino , Polímeros/química , Ratos , Ratos Transgênicos , Ratos Wistar , beta-Ciclodextrinas/química , beta-Ciclodextrinas/farmacologia
4.
Front Microbiol ; 8: 2419, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29270159

RESUMO

Eugenol is a phenolic compound and the main constituent of the essential oil of clove India. Although there are reports of some pharmacological effects of eugenol, this study is the first that proposes to evaluate the antifungal effects of this phenol against both Cryptococcus gattii and C. neoformans cells. The effect of eugenol against yeast cells was analyzed for drug susceptibility, alterations in cell diameter, capsule properties, amounts of ergosterol, oxidative burst, and thermodynamics data. Data demonstrated that there is no interaction between eugenol and fluconazole and amphotericin B. Eugenol reduced the cell diameter and the capsule size, increased cell surface/volume, changed positively the cell surface charge of cryptococcal cells. We also verified increased levels of reactive oxygen species without activation of antioxidant enzymes, leading to increased lipid peroxidation, mitochondrial membrane depolarization and reduction of lysosomal integrity in cryptococcal cells. Additionally, the results showed that there is no significant molecular interaction between eugenol and C. neoformans. Morphological alterations, changes of cellular superficial charges and oxidative stress play an important role in antifungal activity of eugenol against C. gattii and C. neoformans that could be used as an auxiliary treatment to cutaneous cryptococcosis.

5.
Mater Sci Eng C Mater Biol Appl ; 33(7): 3916-22, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23910296

RESUMO

Reaction of highly soluble orally active, non-peptide antihypertensive drug losartan with copper(II) leads to the spontaneous formation of a very insoluble 2:1 covalent complex, which self assembles in a hydrophobic supramolecular structure of nanometric dimensions. Thermal analysis showed that Los/Cu(II) complex presents intermediate stability in comparison with its precursors KLos and Cu(OAc)2·H2O. Isothermal titration calorimetry indicated complexation to be a stepwise process, driven by enthalpy and entropy. Zeta potential and DLS measurements showed that it is possible to control the size and charge of nanoprecipitates by adjusting the relative concentration of Los(-) and Cu(II).


Assuntos
Precipitação Química , Complexos de Coordenação/química , Cobre/química , Interações Hidrofóbicas e Hidrofílicas , Losartan/química , Tamanho da Partícula , Varredura Diferencial de Calorimetria , Espectroscopia de Ressonância de Spin Eletrônica , Cinética , Luz , Dinâmica não Linear , Análise de Regressão , Espalhamento de Radiação , Solubilidade , Soluções , Eletricidade Estática , Termodinâmica , Termogravimetria , Titulometria , Água/química
6.
Beilstein J Org Chem ; 8: 1867-76, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23209524

RESUMO

Organic-inorganic magnetic hybrid materials (MHMs) combine a nonmagnetic and a magnetic component by means of electrostatic interactions or covalent bonds, and notable features can be achieved. Herein, we describe an application of a self-assembled material based on ferrite associated with ß-cyclodextrin (Fe-Ni/Zn/ßCD) at the nanoscale level. This MHM and pure ferrite (Fe-Ni/Zn) were used as an adsorbent system for Cr(3+) and Cr(2)O(7) (2-) ions in aqueous solutions. Prior to the adsorption studies, both ferrites were characterized in order to determine the particle size distribution, morphology and available binding sites on the surface of the materials. Microscopy analysis demonstrated that both ferrites present two different size domains, at the micro- and nanoscale level, with the latter being able to self-assemble into larger particles. Fe-Ni/Zn/ßCD presented smaller particles and a more homogeneous particle size distribution. Higher porosity for this MHM compared to Fe-Ni/Zn was observed by Brunauer-Emmett-Teller isotherms and positron-annihilation-lifetime spectroscopy. Based on the pKa values, potentiometric titrations demonstrated the presence of ßCD in the inorganic matrix, indicating that the lamellar structures verified by transmission electronic microscopy can be associated with ßCD assembled structures. Colloidal stability was inferred as a function of time at different pH values, indicating the sedimentation rate as a function of pH. Zeta potential measurements identified an amphoteric behavior for the Fe-Ni/Zn/ßCD, suggesting its better capability to remove ions (cations and anions) from aqueous solutions compared to that of Fe-Ni/Zn.

7.
Phys Chem Chem Phys ; 14(6): 1934-44, 2012 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-22234498

RESUMO

The size, shape and surface chemistry of nanoparticles play an important role in cellular interaction. Thus, the main objective of the present study was the determination of the ß-cyclodextrin (ß-CD) self-assembly thermodynamic parameters and its structure, aiming to use these assemblies as a possible controlled drug release system. Light scattering measurements led us to obtain the ß-CD's critical aggregation concentration (cac) values, and consequently the thermodynamic parameters of the ß-CD spontaneous self-assembly in aqueous solution: Δ(agg)G(o) = -16.31 kJ mol(-1), Δ(agg)H(o) = -26.48 kJ mol(-1) and TΔ(agg)S(o) = -10.53 kJ mol(-1) at 298.15 K. Size distribution of the self-assembled nanoparticles below and above cac was 1.5 nm and 60-120 nm, respectively. The number of ß-CD molecules per cluster and the second virial coefficient were identified through Debye's plot and molecular dynamic simulations proposed the three-fold assembly for this system below cac. Ampicillin (AMP) was used as a drug model in order to investigate the key role of the guest molecule in the self-assembly process and the ß-CD:AMP supramolecular system was studied in solution, aiming to determine the structure of the supramolecular aggregate. Results obtained in solution indicated that the ß-CD's cac was not affected by adding AMP. Moreover, different complex stoichiometries were identified by nuclear magnetic resonance and isothermal titration calorimetry experiments.


Assuntos
Ampicilina/química , beta-Ciclodextrinas/química , Calorimetria , Portadores de Fármacos/química , Luz , Simulação de Dinâmica Molecular , Nanopartículas/química , Espalhamento de Radiação , Termodinâmica , Fatores de Tempo , Água/química
8.
Colloids Surf B Biointerfaces ; 84(2): 467-76, 2011 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-21376544

RESUMO

This study evaluated the effects of glycolipid-type biosurfactant produced by Trichosporon montevideense CLOA72 in the formation of biofilms in polystyrene plate surfaces by Candida albicans CC isolated from the apical tooth canal. Biofilm formation was reduced up to 87.4% with use of biosurfactant at 16 mg/ml concentration. It has been suggested that the interaction with the cell or polystyrene plate surface could ultimately be responsible for these actions. Therefore, the interaction of C. albicans CC cells with the biosurfactant, as well as the corresponding thermodynamic parameters, have been determined by isothermal titration calorimetry and zeta potential measurements. This process is endothermic (((int)H°=+1284±5 cal/mg OD(600)) occurring with a high increase of entropy (T((int)S°=+10635 cal/mg OD(600)). The caloric energy rate data released during the titulation indicates saturation of the cell-biosurfactant at 1.28 mg/ml OD(600). Also, the zeta potential of the cell surface was monitored as a function of the biosurfactant concentration added to cell suspension showing partial neutralization of net surface charge, since the value of zeta potential ranged from -16 mV to -6 mV during the titration. The changes of cell surface characteristics can contribute to the inhibition of initial adherence of cells of C. albicans in surface. The CMC of the purified biosurfactant produced from T. montevideense CLOA72 is 2.2 mg/ml, as determined both by ITC dilution experiments and by surface tension measurements. This biomolecule did not presented any cytotoxic effect in HEK 293A cell line at concentrations of 0.25-1 mg/ml. This study suggests a possible application of the referred biosurfactant in inhibiting the formation of biofilms on plastic surfaces by C. albicans.


Assuntos
Biofilmes/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Poliestirenos/química , Tensoativos/farmacologia , Trichosporon/metabolismo , Animais , Células HEK293 , Humanos , Espectroscopia de Ressonância Magnética , Propriedades de Superfície , Tensoativos/metabolismo , Termodinâmica
9.
Int J Pharm ; 404(1-2): 116-23, 2011 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-21093554

RESUMO

In this work, low soluble supramolecular complex between the losartan potassium (Los) and hydroxypropil-ß-cyclodextrin (HPßCD) were characterized throughout phase-solubility, NMR techniques ((1)H and 2D-ROESY) and isothermal titration calorimetry (ITC) in order to attain physical-chemical knowledge of the system. In addition, the hypertensive effect of composition Los/HPßCD was evaluated aiming to obtain a more efficient oral pharmaceutical composition. ESI mass spectrometry and ITC blank experiment demonstrate the presence of Los clusters at 30 mM pure solution. Phase-solubility experiments showed a "Bs" type system, due to the formation of a less soluble complex than pure Los. NMR demonstrated the short distance interactions between the Los and the cyclodextrin, where several possibilities of interactions were observed. ITC data suggest an average 1:1 stoichiometry of Los and the cyclodextrin. The complex demonstrated efficiency in hypertension control, presenting antagonist action on the pressure effect of angiotensin II within 30 h, as compared to Los alone, 6h, indicating that inclusion of Los in HPßCD enhanced the extent and duration of its antagonistic action. In this work, a model of interaction between Los and HPßCD was proposed based on dissociation of self-assembled Los followed by complexation with HPßCD.


Assuntos
Bloqueadores do Receptor Tipo 1 de Angiotensina II/farmacologia , Anti-Hipertensivos/farmacologia , Calorimetria , Portadores de Fármacos , Hipertensão/prevenção & controle , Losartan/farmacologia , Espectroscopia de Ressonância Magnética , Espectrometria de Massas por Ionização por Electrospray , Tecnologia Farmacêutica/métodos , alfa-Ciclodextrinas/química , Administração Oral , Angiotensina II , Bloqueadores do Receptor Tipo 1 de Angiotensina II/administração & dosagem , Bloqueadores do Receptor Tipo 1 de Angiotensina II/química , Animais , Anti-Hipertensivos/administração & dosagem , Anti-Hipertensivos/química , Pressão Sanguínea/efeitos dos fármacos , Química Farmacêutica , Modelos Animais de Doenças , Composição de Medicamentos , Hipertensão/induzido quimicamente , Hipertensão/fisiopatologia , Cinética , Losartan/administração & dosagem , Losartan/química , Masculino , Ratos , Ratos Wistar , Solubilidade
10.
Artigo em Inglês | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO | ID: biblio-1064422

RESUMO

O heptapeptídeo BPP7a, p-Glu1Asp2Gly3Pro4Ile5Pro6Pro7, forma um complexo de associaçãocom a β-ciclodextrina na razão molar 1:1. O peptídeo e a sua forma complexa foram caracterizados por dicroísmo circular (CD) e titulação calorimétrica (ITC), as quais sugerem uma interação muito fraca entre a β-ciclodextrina e o peptídeo. Espectros de ressonância magnética nuclear (NMR) de 1H a 400 e 600 MHz foram obtidos para o peptídeo puro e para o complexo com β-ciclodextrina e com estes foi possível a atribuição de todos os sinais de ressonância de hidrogênio do peptídeo. Experimentos de espectroscopia ordenada de difusão de alta resolução (HR-DOSY) foram conduzidos a fim de se confirmar a associação entre o BPP7a e a β-ciclodextrina, além de se verificar a quebra dos agregados moleculares do BPP7a devida a associação. A atividade antihipertensiva do complexo BPP7a/β-ciclodextrina foi avaliada em ratos naturalmente hipertensivos (SHR), mostrando resultados melhores do que os do peptídeo BPP7a puro.


The BPP7a heptapeptide, p-Glu1Asp2Gly3Pro4Ile5Pro6Pro7, forms an association complexwith β-cyclodextrin in a 1:1 molar ratio. The peptide and its complex were characterized bycircular dichroism (CD) and isothermal titration calorimetry (ITC), which showed a very weak interaction between the β-cyclodextrin and the peptide. Assignments of all hydrogen resonances of the peptide alone and as a complex were made using 1H nuclear magnetic resonance (NMR)experiments at 400 and 600 MHz. High resolution diffusion ordered spectroscopy (HR-DOSY) experiments were carried out to establish the self-aggregation state of BPP7a. It was also shown that the β-cyclodextrin breaks the molecular clusters leading to complex formation. In addition,the anti-hypertensive activity of the BPP7a/β-cyclodextrin complex was evaluated in spontaneous hypertensive rats (SHR), showing increased activity compared to that of pure BPP7a.


Assuntos
Animais , Ratos , Análise Espectral/métodos , Espectroscopia de Ressonância Magnética , Espectroscopia de Ressonância Magnética/métodos , Dicroísmo Circular/métodos , Difusão , Ratos Endogâmicos SHR
11.
Carbohydr Res ; 342(15): 2286-96, 2007 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-17618611

RESUMO

The supramolecular assembly between chlorhexidine and cyclomaltoheptaose (beta-cyclodextrin, betaCD) was characterized using NMR spectroscopy ((1)H, T(1), and ROESY), ESIMS and ITC. NMR data suggest the formation of high ordered complexes. ESIMS and ITC allowed the confirmation of the average stoichiometry as 1:4 and the thermodynamic data, also obtained by ITC, showed that the assembly is strongly stabilized by short distance interactions, but suffers a strong, opposite effect of entropy reduction. The antimicrobial activity of 1:1, 1:2, 1:3, and 1:4 Clx/betaCD molar ratio mixtures was investigated in aqueous solution and after incorporation into mucoadhesive gels. These were used to determine the initial and the long-term antimicrobial activity, respectively, toward Actinobacillus actinomycetemcomitans (A.a.) (Y4-FDC) and Enterococcus faecalis (E.f.) (ATCC 14508) strains. The results showed that A.a. and E.f. were more susceptible to the 1:4 molar ratio mixture in either solution or gel (p<0.05).


Assuntos
Anti-Infecciosos Locais/química , Clorexidina/química , Portadores de Fármacos , beta-Ciclodextrinas/química , Actinobacillus/metabolismo , Anti-Infecciosos Locais/administração & dosagem , Calorimetria/métodos , Clorexidina/administração & dosagem , Avaliação Pré-Clínica de Medicamentos , Enterococcus faecalis/metabolismo , Géis/química , Cinética , Espectroscopia de Ressonância Magnética/métodos , Espectrometria de Massas/métodos , Modelos Químicos , Conformação Molecular , Espectrometria de Massas por Ionização por Electrospray/métodos
12.
Int J Pharm ; 336(1): 90-8, 2007 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-17196774

RESUMO

This work describes chemical properties and anti-hypertensive activity of an oral pharmaceutical formulation obtained from the complexation of beta-cyclodextrin (beta-CD) with bradykinin potentiating penta peptide (BPP-5a) founded in the Bothrops jararaca poison. Physical chemistry characterizations were recorded in order to investigate the intermolecular interactions between species in complex. Circular dichroism data indicated conformational changes of BPP-5a upon complexation with beta-CD. ROESY and theoretical calculations showed a selective approximation of triptophan moiety into cavity of beta-CD. Isothermal titration calorimetry data indicated an exothermic formation of the complex, which is accomplished by reduction of entropy. The anti-hypertensive activity of the BPP-5a/beta-CD complex has been evaluated in spontaneous hypertensive rats, showing better results than pure BPP-5a.


Assuntos
Química Farmacêutica/métodos , Oligopeptídeos/química , beta-Ciclodextrinas/química , Inibidores da Enzima Conversora de Angiotensina/química , Inibidores da Enzima Conversora de Angiotensina/farmacologia , Animais , Anti-Hipertensivos/química , Anti-Hipertensivos/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Bothrops , Calorimetria , Dicroísmo Circular , Frequência Cardíaca/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Masculino , Modelos Moleculares , Estrutura Molecular , Oligopeptídeos/farmacologia , Ratos , Ratos Endogâmicos SHR , Termodinâmica , Venenos de Víboras/química
13.
BioDrugs ; 20(5): 283-91, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-17025375

RESUMO

BACKGROUND: Cyclodextrins are suitable drug delivery systems because of their ability to subtly modify the physical, chemical, and biological properties of guest molecules through labile interactions by formation of inclusion and/or association complexes. Plant cysteine proteinases from Caricaceae and Bromeliaceae are the subject of therapeutic interest, because of their anti-inflammatory, antitumoral, immunogenic, and wound-healing properties. METHODS: In this study, we analyzed the association between beta-cyclodextrin (betaCD) and fraction P1G10 containing the bioactive proteinases from Carica candamarcensis, and described the physicochemical nature of the solid-state self-assembled complexes by Fourier transform infrared (FTIR) spectroscopy, thermogravimetry (TG), differential scanning calorimetry (DSC), X-ray powder diffraction (XRD), and nuclear magnetic resonance (NMR), as well as in solution by circular dichroism (CD), isothermal titration calorimetry (ITC), and amidase activity. RESULTS AND DISCUSSION: The physicochemical analyses suggest the formation of a complex between P1G10 and betaCD. Higher secondary interactions, namely hydrophobic interactions, hydrogen bonding and van der Waals forces were observed at higher P1G10 : betaCD mass ratios. These results provide evidence of the occurrence of strong solid-state supramolecular non-covalent interactions between P1G10 and betaCD. Microcalorimetric analysis demonstrates that complexation results in a favorable enthalpic contribution, as has already been described during formation of similar betaCD inclusion compounds. The amidase activity of the complex shows that the enzyme activity is not readily available at 24 hours after dissolution of the complex in aqueous buffer; the proteinase becomes biologically active by the second day and remains stable until day 16, when a gradual decrease occurs, with basal activity attained by day 29. CONCLUSION: The reported results underscore the potential for betaCDs as candidates for complexing cysteine proteinases, resulting in supramolecular arrays with sustained proteolytic activity.


Assuntos
Carica/química , Látex/química , Peptídeo Hidrolases/metabolismo , beta-Ciclodextrinas/química , Amidoidrolases/metabolismo , Calorimetria/métodos , Varredura Diferencial de Calorimetria , Dicroísmo Circular , Espectroscopia de Ressonância Magnética , Espectroscopia de Infravermelho com Transformada de Fourier , Termogravimetria , Difração de Raios X , beta-Ciclodextrinas/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...