RESUMO
OBJECTIVE: To explore the electrophysiological properties of spiral ganglion neurons (SGN) isolated from neonatal mice. METHODS: Ion channel currents of acutely trypsin-dissociated spiral ganglion neurons was recorded and analyzed using whole-cell variation of the voltage clamp technique. RESULTS: In the membrane of SGN, we recorded tetraethylammonium (TEA)-sensitive and 4-aminopyridine (4-AP)-sensitive outside delayed rectifier potassium currents, A-type potassium currents and tetrodotoxin-sensitive inside sodium currents. Ion channel activity had the character of voltage-dependence. Some cells, which did not exhibit sodium currents exhibited, delayed rectifier potassium currents. CONCLUSIONS: The electrophysiological properties of acutely dissociated spiral ganglion neurons could be reference to the research of the mechanics of auditory propagation and the ion channel pharmacology.
Assuntos
Neurônios/fisiologia , Gânglio Espiral da Cóclea/fisiologia , Animais , Células Cultivadas , Eletrofisiologia , Feminino , Canais Iônicos/fisiologia , Masculino , Camundongos , Técnicas de Patch-ClampRESUMO
OBJECTIVE: To conduct a study on the methods for establishing a cytological model of mechanical dissociation and culture of spiral ganglion neurons (SGNs) in postnatal mice. METHODS: The spiral ganglion neurons were taken from 1-6-day old mice for primary culture. The morphology and the process of axon were observed by use of an inverted/phase contrast microscope. Immunocytochemical identification was performed using fluorescent microscopy. RESULTS: In the state of primary culture in vitro, the SGNs were found to have good cell morphology and axon regeneration power, the surviving cells of SGN(5-7 d) amounted to (78.10 +/- 4.33)/cm2 and the survival period was 7-14 d. CONCLUSION: We successfully established the methods of mechanical dissociation and culture of SGN cytological model; the cell amount and survival period of SGNs were able to meet the requirement of cytological experiments.
