Research hotspots and frontiers in agricultural multispectral technology: Bibliometrics and scientometrics analysis of the Web of Science.

Multispectral technology has a wide range of applications in agriculture. By obtaining spectral information during crop production, key information such as growth, pests and diseases, fertilizer and pesticide application can be determined quickly, accurately and efficiently. The scientific analysis based on Web of Science aims to understand the research hotspots and areas of interest in the field of agricultural multispectral technology. The publications related to agricultural multispectral research in agriculture between 2002 and 2021 were selected as the research objects. The softwares of CiteSpace, VOSviewer, and Microsoft Excel were used to provide a comprehensive review of agricultural multispectral research in terms of research areas, institutions, influential journals, and core authors. Results of the analysis show that the number of publications increased each year, with the largest increase in 2019. Remote sensing, imaging technology, environmental science, and ecology are the most popular research directions. The journal Remote Sensing is one of the most popular publishers, showing a high publishing potential in multispectral research in agriculture. The institution with the most research literature and citations is the USDA. In terms of the number of papers, Mtanga is the author with the most published articles in recent years. Through keyword co-citation analysis, it is determined that the main research areas of this topic focus on remote sensing, crop classification, plant phenotypes and other research areas. The literature co-citation analysis indicates that the main research directions concentrate in vegetation index, satellite remote sensing applications and machine learning modeling. There is still a lot of room for development of multi-spectrum technology. Further development can be carried out in the areas of multi-device synergy, spectral fusion, airborne equipment improvement, and real-time image processing technology, which will cooperate with each other to further play the role of multi-spectrum in agriculture and promote the development of agriculture.

Accurate Weed Mapping and Prescription Map Generation Based on Fully Convolutional Networks Using UAV Imagery.

Chemical control is necessary in order to control weed infestation and to ensure a rice yield. However, excessive use of herbicides has caused serious agronomic and environmental problems. Site specific weed management (SSWM) recommends an appropriate dose of herbicides according to the weed coverage, which may reduce the use of herbicides while enhancing their chemical effects. In the context of SSWM, the weed cover map and prescription map must be generated in order to carry out the accurate spraying. In this paper, high resolution unmanned aerial vehicle (UAV) imagery were captured over a rice field. Different workflows were evaluated to generate the weed cover map for the whole field. Fully convolutional networks (FCN) was applied for a pixel-level classification. Theoretical analysis and practical evaluation were carried out to seek for an architecture improvement and performance boost. A chessboard segmentation process was used to build the grid framework of the prescription map. The experimental results showed that the overall accuracy and mean intersection over union (mean IU) for weed mapping using FCN-4s were 0.9196 and 0.8473, and the total time (including the data collection and data processing) required to generate the weed cover map for the entire field (50 × 60 m) was less than half an hour. Different weed thresholds (0.00⁻0.25, with an interval of 0.05) were used for the prescription map generation. High accuracies (above 0.94) were observed for all of the threshold values, and the relevant herbicide saving ranged from 58.3% to 70.8%. All of the experimental results demonstrated that the method used in this work has the potential to produce an accurate weed cover map and prescription map in SSWM applications.

A Semantic Labeling Approach for Accurate Weed Mapping of High Resolution UAV Imagery.

Weed control is necessary in rice cultivation, but the excessive use of herbicide treatments has led to serious agronomic and environmental problems. Suitable site-specific weed management (SSWM) is a solution to address this problem while maintaining the rice production quality and quantity. In the context of SSWM, an accurate weed distribution map is needed to provide decision support information for herbicide treatment. UAV remote sensing offers an efficient and effective platform to monitor weeds thanks to its high spatial resolution. In this work, UAV imagery was captured in a rice field located in South China. A semantic labeling approach was adopted to generate the weed distribution maps of the UAV imagery. An ImageNet pre-trained CNN with residual framework was adapted in a fully convolutional form, and transferred to our dataset by fine-tuning. Atrous convolution was applied to extend the field of view of convolutional filters; the performance of multi-scale processing was evaluated; and a fully connected conditional random field (CRF) was applied after the CNN to further refine the spatial details. Finally, our approach was compared with the pixel-based-SVM and the classical FCN-8s. Experimental results demonstrated that our approach achieved the best performance in terms of accuracy. Especially for the detection of small weed patches in the imagery, our approach significantly outperformed other methods. The mean intersection over union (mean IU), overall accuracy, and Kappa coefficient of our method were 0.7751, 0.9445, and 0.9128, respectively. The experiments showed that our approach has high potential in accurate weed mapping of UAV imagery.

A fully convolutional network for weed mapping of unmanned aerial vehicle (UAV) imagery.

Appropriate Site Specific Weed Management (SSWM) is crucial to ensure the crop yields. Within SSWM of large-scale area, remote sensing is a key technology to provide accurate weed distribution information. Compared with satellite and piloted aircraft remote sensing, unmanned aerial vehicle (UAV) is capable of capturing high spatial resolution imagery, which will provide more detailed information for weed mapping. The objective of this paper is to generate an accurate weed cover map based on UAV imagery. The UAV RGB imagery was collected in 2017 October over the rice field located in South China. The Fully Convolutional Network (FCN) method was proposed for weed mapping of the collected imagery. Transfer learning was used to improve generalization capability, and skip architecture was applied to increase the prediction accuracy. After that, the performance of FCN architecture was compared with Patch_based CNN algorithm and Pixel_based CNN method. Experimental results showed that our FCN method outperformed others, both in terms of accuracy and efficiency. The overall accuracy of the FCN approach was up to 0.935 and the accuracy for weed recognition was 0.883, which means that this algorithm is capable of generating accurate weed cover maps for the evaluated UAV imagery.

DiSNPindel: improved intra-individual SNP and InDel detection in direct amplicon sequencing of a diploid.

BACKGROUND: Amplicon re-sequencing based on the automated Sanger method remains popular for detection of single nucleotide polymorphisms (SNPs) and insertion-deletion polymorphisms (InDels) for a spectrum of genetics applications. However, existing software tools for detecting intra-individual SNPs and InDels in direct amplicon sequencing of diploid samples are insufficient in analyzing single traces and their accuracy is still limited. RESULTS: We developed a novel computation tool, named DiSNPindel, to improve the detection of intra-individual SNPs and InDels in direct amplicon sequencing of a diploid. Neither reference sequence nor additional sample was required. Using two real datasets, we demonstrated the usefulness of DiSNPindel in its ability to improve largely the true SNP and InDel discovery rates and reduce largely the missed and false positive rates as compared with existing detection methods. CONCLUSIONS: The software DiSNPindel presented here provides an efficient tool for intra-individual SNP and InDel detection in diploid amplicon sequencing. It will also be useful for identification of DNA variations in expressed sequence tag (EST) re-sequencing.