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ETHNOPHARMACOLOGICAL RELEVANCE: Jiawei Bai-Hu-Decoction (JWBHD), a prescription formulated with seven traditional Chinese medicinal material has demonstrated clinical efficacy in mitigating brain injury among heat stroke (HS) patients. AIM OF THE STUDY: This study aimed to evaluate the therapeutic efficacy of JWBHD on rat model of HS and to explore its therapeutic mechanisms by integrating network pharmacology and pharmacodynamic methodologies, which major components were analyzed by using UPLC-MS/MS. MATERIALS AND METHODS: The network pharmacology analysis was firstly conducted to predict the potential active ingredients and therapeutic targets of JWBHD. The anti-HS effectiveness of JWBHD was then evaluated on rats experienced HS. Rat brain tissues were harvested for a comprehensive array of experiments, including western blot, PCR, H&E staining, Nissl staining, ELISA, transmission electron microscope, flow cytometry and immunofluorescence to validate the protective effects of JWBHD against HS-induced brain damage. Furthermore, the inhibitory effects of JWBHD on TLR4/NF-κB signal and mitophagy of glial were further verified on HS-challenged F98 cell line. Finally, the chemical compositions of the water extract of JWBHD were analyzed by using UPLC-MS/MS. RESULTS: Network pharmacology has identified fifty core targets and numerous HS-related signaling pathways as potential therapeutic targets of JWBHD. Analysis of protein-protein interaction (PPI) and GO suggests that JWBHD may suppress HS-induced inflammatory signals. In experiments conducted on HS-rats, JWBHD significantly reduced the core temperature, restored blood pressure and alleviated neurological defect. Furthermore, JWBHD downregulated the counts of white blood cells and monocytes, decreased the levels of inflammatory cytokines such as IL-1ß, IL-6 and TNF-α in peripheral blood, and suppressed the expression of TLR4 and NF-κB in the cerebral cortex of HS-rats. Besides, JWBHD inhibited the apoptosis of cortical cells and mitigated the damage to the cerebral cortex in HS group. Conversely, overactive mitophagy was observed in the cerebral cortex of HS-rats. However, JWBHD restored the mitochondrial membrane potential and downregulated expressions of mitophagic proteins including Pink1, Parkin, LC3B and Tom20. JWBHD reduced the co-localization of Pink1 and GFAP, a specific marker of astrocytes in the cerebral cortex of HS-rats. In addition, the inhibitory effect of JWBHD on TLR4/NF-κB signaling and overactive mitophagy were further confirmed in F98 cells. Finally, UPLC-MS/MS analysis showed that the main components of JWBHD include isoliquiritigenin, liquiritin, dipotassium glycyrrhizinate, ginsenoside Rb1, ginsenoside Re, etc. CONCLUSIONS: JWBHD protected rats from HS and prevented HS-induced damage in the cerebral cortex by suppressing TLR4/NF-κB signaling and mitophagy of glial.
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Purpose: This study aims to examine the impact of sleep deprivation on individual cognitive reappraisal ability using a standardized behavioral paradigm. Methods: A randomized pretest-posttest control group design was conducted. Thirty-nine participants were eventually enrolled and randomly assigned to receive either the sleep control (SC: n = 17) or the sleep deprivation (SD: n = 22). Both of them were required to perform a standardized behavioral paradigm of measuring cognitive reappraisal ability one time under sleep-rested condition and another time under the condition of different sleep manipulation a week later. Results: Mean valence ratings of SD group were more negative than SC group's (p < 0.05) and mean arousal ratings of SD group were higher than SC group's (p < 0.01). Conclusion: Sleep deprivation may impair individual cognitive reappraisal ability and could potentially undermine the efficacy of cognitive therapy in terms of emotion regulation.
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The carrying capacity of resources and environment is an essential concept in ecology, the theoretical and practical research of which has become an important basis for measuring regional sustainable development. However, the scientific connection between the ecological foundation and the carrying capacity of resources and environment is still unclear. Moreover, it remains unknown which ecological theories played a supporting role in the development of the resources and environment carrying capacity, which makes the scientific concept of carrying capacity very vague. Based on the discussion of the scientific concepts and development of the carrying capacity of resources and environmen, we systematically discussed the basic concepts, such as the niche volume that organisms can occupy, the ecological threshold of ecosystems to withstand environmental stress, the potential resource capacity (supply capacity) of sustainable supply such as climate, water and nutrition, and the environmental capacity of buffering and purifying pollutants. Furthermore, from the biophysical point perspective of foundation pressure bearing capacity, spatial capacity carrying capacity and ecological threshold carrying capacity, the scientific concepts of Natural Resources Supply Carrying Capacity (NRSCC), Nature Environment Carrying Capacity (NECC), Carrying Capacity of Biological Population Development (CCBPD), Carrying Capacity of Social and Economic Development (CCSED) and Carrying Capacity of Environmental Stress in Ecosystems (CCESE) were defined. Finally, three basic issues of ecology were discussed in detail, including the theory of population growth and the ecological capacity of the ecosystem, the theory of ecosystem multi-functionality and resource and environmental effects, and the theory of alternative stable states, self-adaptability, and self-organization. Based on exploring the theory and method of regional resource environmental assessment, this study would provide theoretical basis for regional resource environment utilization, protection and social and economic sustainable development.
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Conservação dos Recursos Naturais , Ecossistema , China , Ecologia , Desenvolvimento Econômico , Desenvolvimento Sustentável , ÁguaRESUMO
Our understanding of resources and environmental carrying capacity is deepened with the comprehensive effects of human needs and external stress. When human survival and development mainly depend on the supply of local resources and environmental conditions, the resources and environmental carrying capacity is largely controled by the dominant limiting factors. With sustainable development and environmental protection, the resources and environmental carrying capacity has gradually changed from supply restriction to demand support. There is an expression of capacity, threshold, intensity, and ability to characterize the resources and environmental carrying capacity. The impact of climate change and human activities on the resource and environmental system is increasing, altering resources and environmental carrying capacity. At present, the interrelationship and internal mechanism among resource and environmental carrying capacity, ecosystem vulnerability, and climate change risk are still unclear, which restricts the further development of theory and method. We preliminarily summarized and discussed the basic theory and method system of the research on carrying capacity of regional resources and environment. Furthermore, we advocated to develop the cascade relations of "carrying capacity of biological population development-carrying capacity of environmental stress in ecosystems-natural resources supply carrying capacity-natural environment carrying capacity-carrying capacity of social and economic development". Moreover, the calculation method and conceptual model of multi-dimensional resource and environmental carrying capacity were put forward under each concept framework. This study provided new ideas for the research on the method of resources and environment carrying capacity.
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Conservação dos Recursos Naturais , Ecossistema , China , Desenvolvimento Econômico , Humanos , Modelos Teóricos , Desenvolvimento SustentávelRESUMO
Recently, the cellular origin of the connecting tubule (CNT) has been genetically characterized. The CNT is a segment between two embryonically different structures, the collecting duct originating from ureteric bud (UB), and the nephron derived from the cap mesenchyme. However, the cellular detail at the initial connection is limited. The present study demonstrated that the initial connection was composed of cells which were closely associated with the renal vesicle (RV), the initial nephron, and connected with the basal epithelium of the terminal UB tip at discrete points. The identification of the RV and UB tip was based on tracing of tubules on serial epoxy sections at mouse embryonic day 17.5. The cells at the initial connection were characterized by 1) irregularly-shaped nuclei and cells with cytoplasmic processes, 2) electron dense nuclei, 3) abundant intercellular spaces, 4) extensive cell-cell contacts with cell junctions, often zonulae adherences and occasionally focal fusion of opposing plasma membranes, and 5) numerous mitochondria, densely packed rosette-like polyribosomes, and widespread rER in the cytoplasm. Moreover, the tracing revealed that a terminal UB tip frequently connected to two nephrons at different developing stages. The UB tips, the initial connections, and the distal tubules of the S-shaped bodies did not express Na+-Cl- cotransporter, H+-ATPase, or aquaporin 2, while they were expressed in immature CNT of the capillary-loop stage nephrons throughout the kidney development. Consequently, the cells at the initial connection exhibit the morphological features suggestive of energy demanding, protein producing, and intercellular communicating. The cell morphology together with transporter development indicates that these cells serve several functions during the development of the initial connection, and that these functions are different from the cells' final functions as transportation.
