Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 19 de 19
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Fitoterapia ; 136: 104155, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31028819

RESUMO

In the quest to search and discover bioactive compounds from nature, terpenoids have emerged as one of the most interesting and researched classes of compounds. Secoiridoid, a type of the terpenoid, has also been extensively studied, especially their chemical structures and pharmacological effects. Oleaceae is a family of woody dicotyledonous plants with broad economic and medicinal values. This family contains a large number of flavonoids, monoterpenoids, iridoids, secoiridoids and phenylethyl alcohols, of which the secoiridoids have various biological activities. The purpose of this review is to summarize the phytochemical and pharmacological of the secoiridoids (glycosides, aglycones, derivatives and dimers) in the Oleaceae family from 1987 to 2018. This review will also serve as a reference for further studies.


Assuntos
Iridoides/química , Iridoides/farmacologia , Oleaceae/química , Animais , Flavonoides , Glicosídeos , Humanos , Estrutura Molecular , Monoterpenos , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia
2.
Molecules ; 24(6)2019 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-30917586

RESUMO

The purpose of this research was to extract and separate the compounds from frankincense, and then evaluate their anti-inflammatory effects. The isolated compound was a representative tetracyclic triterpenes of glycine structure according to ¹H-NMR and 13C-NMR spectra, which is ß-elemonic acid (ß-EA). We determined the content of six different localities of frankincense; the average content of ß-EA was 41.96 mg/g. The toxic effects of ß-EA administration (400, 200, 100 mg/kg) for four weeks in Kunming (KM) mice were observed. Compared with the control group, the body weight of mice, the visceral coefficients and serum indicators in the ß-EA groups showed no systematic variations. The anti-inflammatory effects of ß-EA were evaluated in LPS-induced RAW264.7 cells, xylene-induced induced ear inflammation in mice, carrageenin-induced paw edema in mice, and cotton pellet induced granuloma formation in rats. ß-EA inhibited overproduction of tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP-1), soluble TNF receptor 1 (sTNF R1), Eotaxin-2, Interleukin 10 (IL-10) and granulocyte colony-stimulating factor (GCSF) in the RAW264.7 cells. Intragastric administration with ß-EA (300, 200, and 100 mg/kg in mice, and 210, 140, and 70 mg/kg in rats) all produced distinct anti-inflammatory effects in vivo in a dose-dependent manner. Following treatment with ß-EA (300 mg/kg, i.g.), the NO level in mice ears and PGE2 in mice paws both decreased (p < 0.01). In conclusion, our study indicates that ß-EA could be a potential anti-inflammatory agent for the treatment of inflammatory diseases.


Assuntos
Anti-Inflamatórios/administração & dosagem , Dinoprostona/metabolismo , Franquincenso/química , Inflamação/tratamento farmacológico , Triterpenos/administração & dosagem , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Carragenina/efeitos adversos , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Técnicas In Vitro , Inflamação/induzido quimicamente , Lipopolissacarídeos/efeitos adversos , Camundongos , Óxido Nítrico/metabolismo , Células RAW 264.7 , Ratos , Triterpenos/química , Triterpenos/farmacologia , Xilenos/efeitos adversos
3.
Int J Biol Macromol ; 119: 677-682, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30067956

RESUMO

2,3,5,4'-Tetrahydroxy-stilbene-2-O-ß-d-glucoside (THSG) is a bioactive polyhydroxystilbene compound obtained from Polygonum multiflorum Thunb. Although it has broad therapeutic potential, the application of THSG is limited due to its poor stability in vitro, poor absorption in the intestine, and short-term storage in tissues. Polysaccharides from herbal medicines have been proven to be functional foods and potential natural plant derived drugs. However, the effects of polysaccharides when co-administered with other active ingredients are currently unknown. The present study investigated the influences of Ophiopogon japonicus polysaccharides (OJPs) on THSG. Results showed that OJPs notably enhanced aqueous solubility, and stability of THSG, but slightly decreased the permeability of THSG. In addition, Tmax, Cmax, and AUC(0-tn) of THSG were 3.5 fold, 1.45 fold and 2.32 fold higher for THSG-OJP. Thus, OJPs could potentially be used to improve the biopharmaceutical properties and prolong the pharmacological effects of THSG. This finding could provide a reference point for further applications of polysaccharides from herbal medicines.


Assuntos
Glucosídeos/farmacologia , Glucosídeos/farmacocinética , Ophiopogon/química , Polissacarídeos/farmacologia , Estilbenos/farmacologia , Estilbenos/farmacocinética , Interações Medicamentosas , Glucosídeos/química , Permeabilidade , Solubilidade , Estilbenos/química
4.
J Food Drug Anal ; 26(1): 90-99, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29389593

RESUMO

Polygoni Multiflori Radix (PMR) is increasingly being used not just as a traditional herbal medicine but also as a popular functional food. In this study, multivariate chemometric methods and mass spectrometry were combined to analyze the ultra-high-performance liquid chromatograph (UPLC) fingerprints of PMR from six different geographical origins. A chemometric strategy based on multivariate curve resolution-alternating least squares (MCR-ALS) and three classification methods is proposed to analyze the UPLC fingerprints obtained. Common chromatographic problems, including the background contribution, baseline contribution, and peak overlap, were handled by the established MCR-ALS model. A total of 22 components were resolved. Moreover, relative species concentrations were obtained from the MCR-ALS model, which was used for multivariate classification analysis. Principal component analysis (PCA) and Ward's method have been applied to classify 72 PMR samples from six different geographical regions. The PCA score plot showed that the PMR samples fell into four clusters, which related to the geographical location and climate of the source areas. The results were then corroborated by Ward's method. In addition, according to the variance-weighted distance between cluster centers obtained from Ward's method, five components were identified as the most significant variables (chemical markers) for cluster discrimination. A counter-propagation artificial neural network has been applied to confirm and predict the effects of chemical markers on different samples. Finally, the five chemical markers were identified by UPLC-quadrupole time-of-flight mass spectrometer. Components 3, 12, 16, 18, and 19 were identified as 2,3,5,4'-tetrahydroxy-stilbene-2-O-ß-d-glucoside, emodin-8-O-ß-d-glucopyranoside, emodin-8-O-(6'-O-acetyl)-ß-d-glucopyranoside, emodin, and physcion, respectively. In conclusion, the proposed method can be applied for the comprehensive analysis of natural samples.


Assuntos
Cromatografia Líquida de Alta Pressão , Gastrópodes/química , Gastrópodes/classificação , Metabolômica , Animais , Espectrometria de Massas , Metabolômica/métodos , Estrutura Molecular
5.
J Asian Nat Prod Res ; 19(10): 981-986, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28276764

RESUMO

Two new malic acid derivatives, namely eucomic acid 1-methyl ester (2) and 6'''-acetylmilitaline (7), together with ten known compounds (1, 3-6, 8-12), were isolated from the dry tubers of Bletilla striata (Thunb.) Reichb. F., a perennial traditional Chinese medicinal herb, which was used for the treatment of pneumonophthisis, pneumonorrhagia, tuberculosis, and hemorrhage of the stomach or lung. Their structures were elucidated by spectroscopic analyses, including 1D-, 2D-NMR, and HR-ESI-MS.


Assuntos
Medicamentos de Ervas Chinesas/isolamento & purificação , Malatos/isolamento & purificação , Orchidaceae/química , Fenóis/isolamento & purificação , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Malatos/química , Malatos/farmacologia , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Fenóis/química , Fenóis/farmacologia , Tubérculos/química
6.
Oncotarget ; 7(25): 38292-38305, 2016 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-27203677

RESUMO

Tumor-associated macrophages, crucial components of the microenvironment in hepatocellular carcinoma, hamper anti-cancer immune responses. The aim of the present study was to investigate the effect of sorafenib on the formation of the tumor microenvironment, especially the relationship between polarized macrophages and hepatocytes. Macrophage infiltration was reduced in patients with hepatocellular carcinoma who were treated with sorafenib. In vitro, sorafenib abolished polarized macrophage-induced epithelial mesenchymal transition (EMT) and migration of hepatocellular carcinoma cells but not normal hepatocytes. Moreover, sorafenib attenuated HGF secretion in polarized macrophages, and decreased plasma HGF in patients with hepatocellular carcinoma. Additionally, sorafenib abolished the polarized macrophage-induced activation of the HGF receptor Met in hepatocellular carcinoma cells. Our findings suggest that sorafenib inhibits polarized macrophage-induced EMT in hepatocellular carcinoma cells via the HGF-Met signaling pathway. These results contribute to our understanding of the immunological mechanisms that underlie the protective effects of sorafenib in hepatocellular carcinoma therapy.


Assuntos
Antineoplásicos/uso terapêutico , Carcinoma Hepatocelular/tratamento farmacológico , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Neoplasias Hepáticas/tratamento farmacológico , Macrófagos/efeitos dos fármacos , Niacinamida/análogos & derivados , Compostos de Fenilureia/uso terapêutico , Idoso , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Humanos , Neoplasias Hepáticas/patologia , Macrófagos/patologia , Masculino , Niacinamida/uso terapêutico , Transdução de Sinais , Sorafenibe
7.
Zhong Yao Cai ; 39(5): 991-5, 2016 May.
Artigo em Chinês | MEDLINE | ID: mdl-30132635

RESUMO

Objective: To establish an UHPLC method for simultaneous determination of six flavonoid components of three closelyrelated plants Agrimonia pilosa,Potentilla chinensis and Potentilla discolor including rutin,hyperoside,cynaroside,quercetin,apigenin and kaempferol. Meanwhile three fresh and dry plants were evaluated to compare the contents of six flavonoid components. Methods: The samples were pretreated with ultrasonic extraction with 70% ethanol for 0. 5 h. The analysis was performed on an Acquity HSS T3( 100 mm × 3. 0 mm,1. 8 µm) column with the mobile phase consisting of acetonitrile and 0. 3% glacial acetic acid aqueous at a flow rate of0. 4 m L / min. The detection wavelength was 360 nm,and the column temperature was 35 ℃. Results: The contents of rutin and hyperoside were high generally,but the content of kaempferol was extremely low in three closely-related plants. The content of apigenin was0. 028 mg / g in Potentilla chinensis,but not detected in Agrimonia pilosa and Potentilla discolor. The content of cymaroside in Agrimonia pilosa was significantly higher than that in Potentilla chinensis and Potentilla discolor. The fresh plants of Potentilla chinensis and Potentilla discolor contained more flavonoids than oven drying plants. Conclusion: The similar trend of content change from fresh to dry plant has showed a chemotaxonomic relationship of Potentilla chinensis and Potentilla discolor. The established determination method is simple,rapid and efficient,and is applicable for analysis of the contents of flavonoids in three closely-related plants,which provides the scientific basis for rationalization of using these drugs in clinic.


Assuntos
Agrimonia , Potentilla , Acetonitrilas , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas , Flavonoides , Glucosídeos , Quempferóis , Luteolina , Quercetina/análogos & derivados , Rutina
8.
Carbohydr Polym ; 132: 437-43, 2015 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-26256368

RESUMO

Xue-Zhi-Ning (XZN) is a traditional Chinese medicine formula, containing active ingredients with poor solubility in water, which has been demonstrated to be helpful for patients with hyperlipidemia. One-pot ß-cyclodextrin (ß-CD)-assisted extraction of active ingredients from XZN has been carried out to develop an efficient and eco-friendly extraction process. Five active compounds--rubrofusarin gentiobioside, 2,3,5,4'-tetrahydroxy-stilbene-2-O-ß-D-glucoside, emodin, nuciferine and quercetin--were identified by UPLC/DAD/MS and used as indexes to evaluate the process optimized by an orthogonal test. The results showed that addition of ß-CD significantly enhanced the extraction ratios of all five components. The enhancement of extraction ratios was positively correlated with the apparent formation constants between ß-CD and the compounds. The study also showed that the stabilities and dissolution rates of the active ingredients were improved in the presence of ß-CD. This one-pot ß-cyclodextrin-assisted extraction has the potential to be applied in pharmaceutical preparations directly.


Assuntos
Medicamentos de Ervas Chinesas/análise , beta-Ciclodextrinas/química , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/isolamento & purificação , Concentração de Íons de Hidrogênio , Espectrometria de Massas , Medicina Tradicional Chinesa , Solubilidade , Água
9.
Yao Xue Xue Bao ; 50(5): 587-93, 2015 May.
Artigo em Chinês | MEDLINE | ID: mdl-26234142

RESUMO

In order to identify the chemical constituents of Yushu tablets comprehensively, we studied the chemical constituents of CHCl3 extract from Yushu tablets by the ultra performance liquid chromatography-electrospray ionization-ion trap-time of flight mass spectrometry (UPLC-ESI-IT-TOF/MS). It showed that there were more than 100 compounds separated, and forty-nine peaks among these were identified on the basis of high resolution mass spectrometry data and literature data reported. Determination of twelve peaks was further confirmed by standard substances. These components assigned to the different plant sources mainly included phenylpropanoids, triterpenoids, quinones and m-trihydroxybenzene compounds. By analyzing the chemical components of CHCl3 extract from compound Chinese medicine Yushu tablets comprehensively, this study provided the foundation for studying chemical components, pharmacodynamic substance and quality control of Yushu tablets.


Assuntos
Medicamentos de Ervas Chinesas/análise , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Espectrometria de Massas por Ionização por Electrospray , Comprimidos , Espectrometria de Massas em Tandem
10.
Zhong Yao Cai ; 38(1): 156-9, 2015 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-26214884

RESUMO

OBJECTIVE: To develop an HPLC-ELSD method for simultaneous determination of Astragaloside IV Astragaloside I, Astragaloside II, Astragaloside III and Isostragaloside II in Astragali Radix and Jinqi Jiangtang tablet. METHODS: The chromatographic conditions were as follows: Grace Apollo C18 column (250 mm x 4. 6 mm, 5 µm), acetonitrile (A) and water(B) as mobile phases for gradient elution, and the flow rate being 1. 0 mL/min. RESULTS: Five components showed good linearity. The average recoveries were between 95% - 105%. Five Astragalosides were determined in twelve batches of Astragali Radix and ten batches of Jinqi Jiangtang tablet. CONCLUSION: This is a specific, sensitive and simple method for simultaneous determination of Astragaloside IV, Astragaloside I Astragaloside II, Astragaloside III and Isostragaloside II in Astragali Radix and Jinqi Jiangtang tablet.


Assuntos
Astrágalo/química , Medicamentos de Ervas Chinesas/química , Saponinas/análise , Triterpenos/análise , Astragalus propinquus , Cromatografia Líquida de Alta Pressão , Saponinas/isolamento & purificação , Comprimidos , Triterpenos/isolamento & purificação
11.
Int J Oncol ; 44(1): 247-55, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24190572

RESUMO

Mortalin is highly expressed in a variety of human tumors and associated with tumor metastasis. However, the relationship among the overexpression of Mortalin, epithelial to mesenchymal transition (EMT) and neovascularization is largely unknown. The aim of the present study was to investigate the expression of Mortalin in human HCC cell lines, clinical HCC specimens and its association with angiogenesis and EMT. The results of our study showed that the expression levels of Mortalin in cell lines with higher metastatic potential were significantly higher compared to those with lower metastatic potential. Compared with paracarcinomatous tissues and normal liver tissues, the expression of Mortalin was significantly increased in HCC tumor tissues. The expression of Mortalin was correlated with invasion and metastasis, Edmondson grade and TNM stage. A significant positive correlation was found between the expression of Mortalin and Vimentin, and tumors with high expression of Mortalin had a tendency to higher MVD compared to those with low expression of Mortalin. Using shRNA-mediated Mortalin knockdown, we found that decreased expression of Mortalin was accompanied by a reduction of Vimentin expression. Our findings demonstrated that the overexpression of Mortalin is correlated with the metastatic phenotype of HCC cells and can promote EMT, but cannot induce angiogenesis in HCC. The decreased expression of Mortalin is accompanied by an inhibition of EMT in the HCC cell lines.


Assuntos
Carcinoma Hepatocelular/genética , Proteínas de Choque Térmico HSP70/biossíntese , Neoplasias Hepáticas/genética , Neovascularização Patológica/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Transição Epitelial-Mesenquimal/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias
12.
J Autoimmun ; 46: 25-34, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23948302

RESUMO

There have been major advances in defining the immunological events associated with fibrosis in various chronic liver diseases. We have taken advantage of this data to focus on the mechanisms of action of a unique multi-kinase inhibitor, coined sorafenib, on CCl4-induced murine liver fibrosis, including the effects of this agent in models of both acute and chronic CCl4-mediated pathology. Importantly, sorafenib significantly attenuated chronic liver injury and fibrosis, including reduction in liver inflammation and histopathology as well as decreased expression of liver fibrosis-related genes, including α-smooth muscle actin, collagen, matrix metalloproteinases and the tissue inhibitor of metalloproteinase-1. Furthermore, sorafenib treatment resulted in translocation of cytoplasmic STAT3 to the nucleus in its active form. Based on this observation, we used hepatocyte-specific STAT3 knockout (STAT3(Hep-/-)) mice to demonstrate that hepatic STAT3 was critical for sorafenib-mediated protection against liver fibrosis, and that the upregulation of STAT3 phosphorylation was dependent on Kupffer cell-derived IL-6. In conclusion, these data reflect the clinical potential of the multi-kinase inhibitor sorafenib for the prevention of fibrosis as well as the treatment of established liver fibrosis and illustrate the immunological mechanisms that underlie the protective effects of sorafenib.


Assuntos
Cirrose Hepática/prevenção & controle , Fígado/efeitos dos fármacos , Niacinamida/análogos & derivados , Compostos de Fenilureia/farmacologia , Fator de Transcrição STAT3/metabolismo , Actinas/genética , Actinas/metabolismo , Transporte Ativo do Núcleo Celular/efeitos dos fármacos , Animais , Tetracloreto de Carbono , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/metabolismo , Células Cultivadas , Colágeno Tipo I/genética , Colágeno Tipo I/metabolismo , Cadeia alfa 1 do Colágeno Tipo I , Expressão Gênica/efeitos dos fármacos , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Hepatócitos/patologia , Immunoblotting , Imuno-Histoquímica , Interleucina-6/genética , Interleucina-6/metabolismo , Cinética , Células de Kupffer/efeitos dos fármacos , Células de Kupffer/metabolismo , Células de Kupffer/patologia , Fígado/metabolismo , Fígado/patologia , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Niacinamida/farmacologia , Fosforilação/efeitos dos fármacos , Inibidores de Proteínas Quinases/farmacologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fator de Transcrição STAT3/genética , Sorafenibe , Inibidor Tecidual de Metaloproteinase-1/genética , Inibidor Tecidual de Metaloproteinase-1/metabolismo
13.
J Pharm Biomed Anal ; 77: 71-5, 2013 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-23384552

RESUMO

A rapid and sensitive bioassay based on liquid chromatography tandem mass spectrometry (LC-MS/MS) has been developed and validated for the simultaneous determination of eight coumarins in rat plasma. The liquid-liquid extraction method with ethyl acetate was used to prepare the plasma samples after addition of warfarin as an internal standard (IS). Chromatographic separation was performed on an Eclipse plus C18 column (100mm×4.6mm, 1.8µm) using gradient elution when 1mM ammonium acetate aqueous solution - acetonitrile was used as the mobile phase. The lower limit of quantitation (LLOQ) of each coumarin was lower than 2.16ngmL(-1). Intra-day and inter-day precisions were less than 15%. The accuracies were in the range of 88.9-117%. The mean recoveries of coumarins and IS were higher than 84%. The method was successfully applied to a pharmacokinetic study of eight coumarins in rats after oral administration of radix angelicae pubescentis.


Assuntos
Cumarínicos/sangue , Ficusina/sangue , Furocumarinas/sangue , Metoxaleno/análogos & derivados , Metoxaleno/sangue , Escopoletina/sangue , 5-Metoxipsoraleno , Acetatos/química , Administração Oral , Animais , Cromatografia Líquida/métodos , Cumarínicos/química , Cumarínicos/farmacocinética , Medicamentos de Ervas Chinesas/química , Ficusina/química , Ficusina/farmacocinética , Furocumarinas/química , Furocumarinas/farmacocinética , Extração Líquido-Líquido/métodos , Masculino , Metoxaleno/química , Metoxaleno/farmacocinética , Extratos Vegetais/química , Raízes de Plantas/química , Ratos , Ratos Sprague-Dawley , Escopoletina/química , Escopoletina/farmacocinética , Espectrometria de Massas em Tandem/métodos
14.
Clin Rev Allergy Immunol ; 44(3): 229-41, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22826112

RESUMO

Traditional Chinese medicines (TCMs) have a long history in Asian countries and are traditionally used to prevent and treat a variety of diseases. The rising interest in TCMs in recent years is reflected in both the increase in their market demand as well as scientific research. Previous studies show that TCMs perform dual roles on immunological regulation: immunological activation and immunological suppression. This review highlights studies focusing on the immunomodulatory effects of TCMs, describing their stimulatory effect on immune cells, immune organs, cytokine production, tumorigenesis, as well as their inhibitory function on inflammation, allergy, autoimmune disease, and graft rejection. Components of both innate and adaptive immunity may be modulated by specific TCMs. TCMs may also have antitumor effects and may play a role in regulating apoptosis. Immunomodulatory effects of TCMs may lead to new medications to treat allergic and autoimmune diseases. More high quality studies are needed to achieve scientific validity to these potential treatments. Evidence presented in this review reveals the role of TCMs in immune regulation and proposes a promising future for them in immunomodulatory therapies.


Assuntos
Imunomodulação , Medicina Tradicional Chinesa , Animais , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Humanos , Fatores Imunológicos/farmacologia , Fatores Imunológicos/uso terapêutico , Imunomodulação/efeitos dos fármacos
16.
Zhongguo Zhong Yao Za Zhi ; 33(7): 775-7, 2008 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-18589777

RESUMO

OBJECTIVE: To study the chemical constituents of the whole plant Caragana spinifera. METHOD: The chemical constituents were isolated and repeatedly purified on silica gel column. The structures were elucidated by the NMR spectra and physico-chemical properties. RESULT: Six compounds were isolated and identified as (6aR, 11aR) 4,9-dimethoxy-3-hydroxypterocarpan, (6aR,11aR)-4, 9-dihydroxy-3- methoxypterocarpan (melilotocarpane B), 5, 4'-dihydroxy-7-methoxyisoflavone, 7-hydroxy4'-methoxyisoflavone, 6, 7-dihydroxy4'-methoxyisoflavone, beta-sitosterol respectively. CONCLUSION: All compounds were isolated from the plant for the first time.


Assuntos
Caragana/química , Cristalografia por Raios X , Medicamentos de Ervas Chinesas/química , Isoflavonas/análise , Isoflavonas/química , Isoflavonas/isolamento & purificação , Espectroscopia de Ressonância Magnética , Pterocarpanos/análise , Pterocarpanos/química , Pterocarpanos/isolamento & purificação
17.
Zhongguo Zhong Yao Za Zhi ; 30(4): 272-4, 2005 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-15724404

RESUMO

OBJECTIVE: To study the chemical constituents from Lamium maculatum var. kansuense. METHOD: The chemical constituents were isolated and repeatedly purified on silica gel column and the structures were elucidated by the NMR spectra and physico-chemical properties. RESULT: Six compounds were obtained and identified as polypodine B (I), 5-OH-8-epiloganin (II), shlanzhiside methyl ester (III), liriodendrin (IV), quercitroside (V), uridine (VI). CONCLUSION: Compound IV was found from genus Lamium for the first time and the rest of the compounds were found from Lamium maculatum var kansuense for the first time.


Assuntos
Ecdisterona/análogos & derivados , Ecdisterona/isolamento & purificação , Furanos/isolamento & purificação , Glucosídeos/isolamento & purificação , Lamiaceae/química , Plantas Medicinais/química , Ecdisterona/química , Furanos/química , Glucosídeos/química , Uridina/química , Uridina/isolamento & purificação
18.
Zhongguo Zhong Yao Za Zhi ; 28(8): 730-2, 2003 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-15015352

RESUMO

OBJECTIVE: To study the chemical constituents from Lamium maculatum L. var Kansuense. METHOD: The chemical constituents were isolated and repeatedly purified by silica gel column chromatography and the structures were elucidated by the NMR spectra and physico-chemical properties. RESULT: Ten compounds were obtained and they were identified as D-mannitol, beta-sitosterol, stigmasterol, rutin, 3'-methylquercetin-3-O-rutinoside, n-butyl-beta-D-fructopyranoside, daucosterol, acteoside, 20-hydroxyecdysone, allantoin. CONCLUSION: All the compounds were obtained from L. maculatum L. var Kansuense for the first time.


Assuntos
Lamiaceae/química , Manitol/isolamento & purificação , Plantas Medicinais/química , Sitosteroides/isolamento & purificação , Alantoína/química , Alantoína/isolamento & purificação , Ecdisterona/química , Ecdisterona/isolamento & purificação , Glucosídeos/química , Glucosídeos/isolamento & purificação , Manitol/química , Fenóis/química , Fenóis/isolamento & purificação , Rutina/química , Rutina/isolamento & purificação , Sitosteroides/química , Estigmasterol/química , Estigmasterol/isolamento & purificação
19.
Zhongguo Zhong Yao Za Zhi ; 28(7): 627-9, 2003 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-15139106

RESUMO

OBJECTIVE: To provide the foundation for reasonable utilization by analyzing the essential oils of Ligularia virgaurea. METHOD: The essential oils were extracted by using steam distillation and separated with GC capillary columns. The components were quantitatively determined with normalization method, and were identified with GC-MS. RESULT: 41 components were identified, which took up 72.73% of the essential oils. CONCLUSION: The main components of essential oils were 4-methyl-1-(1-methylethyl)-3-cyclohexen-1-ol(14.369%), crotonic acid, 2,2-dimethyl-butanoic acid, 1-methyl-3-(1-methylethyl)-benzene, (1s-endo)-1,7,7-trimethyl-bicyclo[2,2,1]heptan-2-ol, trans-1-methyl-4-(1-methylethyl)-2-cyclohexen-1-ol, alpha-cadinol and alpha,alpha,4-trimethyl-3-cyclohexene-1-methanol.


Assuntos
Asteraceae/química , Óleos Voláteis/química , Plantas Medicinais/química , Butiratos/análise , Crotonatos/análise , Cromatografia Gasosa-Espectrometria de Massas , Óleos Voláteis/isolamento & purificação , Óleos de Plantas/química , Óleos de Plantas/isolamento & purificação , Terpenos/análise
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...