RESUMO
AIM: Despite the fact that angiotensin (Ang) II is a critical regulator of the proliferation and migration of vascular smooth muscle cells (VSMCs), the effect of Ang II on VSMC proliferation has remained unclear. In this study, we determined whether Stim1- and Orai1-mediated store-operated calcium (Ca(2+)) entry (SOCE) plays a critical role in Ang II-induced VSMC proliferation and Ang II-accelerated neointimal growth after balloon injury of rat carotid arteries. METHODS AND RESULTS: Knockdown of Stim1 and Orai1, putative calcium sensors/modulators, suppressed Ang II-mediated Ca(2+) entry and cell proliferation in synthetic VSMCs. Stim1 and Orai1 short interfering RNAs (siRNAs) decreased neointimal growth induced by Ang II in balloon-injured rat carotid arteries. Ang II signiï¬cantly increased the expression of Stim1 and Orai1 in neointima. In addition, our results showed that receptor subtype-1 (AT1) significantly contributed to Ang II-induced Ca(2+) entry and proliferation of synthetic VSMCs. However, we found that transient receptor potential canonical 1 (Trpc1) had no effect on Ang II-induced SOCE or cell proliferation of synthetic VSMCs. CONCLUSIONS: We show for the first time that Stim1- and Orai1-mediated SOCE may be critical for Ang II-induced VSMC proliferation. This provides important information with respect to targeting cardiovascular diseases under the enhanced renin-Ang system.
Assuntos
Angiotensina II/farmacologia , Canais de Cálcio/fisiologia , Cálcio/metabolismo , Proliferação de Células/efeitos dos fármacos , Glicoproteínas de Membrana/fisiologia , Músculo Liso Vascular/efeitos dos fármacos , Miócitos de Músculo Liso/efeitos dos fármacos , Animais , Masculino , Músculo Liso Vascular/metabolismo , Músculo Liso Vascular/patologia , Miócitos de Músculo Liso/metabolismo , Miócitos de Músculo Liso/patologia , Proteína ORAI1 , Ratos , Ratos Wistar , Receptor Tipo 1 de Angiotensina/fisiologia , Molécula 1 de Interação EstromalRESUMO
The normal and malignant tissues from hypothyroid were investigated by FT-Raman spectroscopy. The results show that two characteristic Raman spectral peaks of normal tissues appeared at 503 and 758 cm(-1) (corresponding to theextension vibration of C--I band and the vibration of benzene rings respectively) in the range of 400-3500 cm(-1). The two peaks disappeared at malignant tissues. The intensity of the characteristic vibration peaks of normal tissues appearing at 3 062 and 1 003 cm(-1) corresponding to tyrosine weakened in malignant tissues. Thus, FT-Raman spectroscopy is a potential method in the diagnosis of hypothyroid cancer.
