Effects of Secondary Metabolites of Rice on Brown Planthopper and Its Symbionts.

The brown planthopper Nilaparvata lugens (Stål) (BPH) is a main rice pest in China and many other Asian countries. In the control of BPH, the application of insect-resistant rice has proven to be quite effective. Secondary metabolites are essential weapons in plants' defense against phytophagous insects. Studies have found that differences in the content of secondary metabolites play a crucial role in determining whether rice exhibits resistance or susceptibility to BPH. Simultaneously, symbionts are essential to the BPH. Nevertheless, there is limited research on the impact of secondary metabolites on the symbionts within BPH. Therefore, investigating the influence of secondary metabolites on both BPH and their symbionts is significant for the control of BPH. In this experiment, newly emerged female adults of BPH were fed artificial diets containing 10 different secondary metabolites. The results indicated that methyl jasmonate had inhibitory effects on the survival rate, weight gain, and reproductive capacity of BPH. Using qPCR methods, it was discovered that the number of symbiotic fungi (Ascomycetes symbionts) within BPH significantly decreased under methyl jasmonate stress. In conclusion, this experiment has preliminarily revealed the inhibitory effects of methyl jasmonate on BPH and its symbionts, demonstrating its potential for controlling BPH.

Preferential Subgenome Elimination and Chromosomal Structural Changes Occurring in Newly Formed Tetraploid Wheat-Aegilops ventricosa Amphiploid (AABBDvDvNvNv).

Artificial allopolyploids derived from the genera Triticum and Aegilops have been used as genetic resources for wheat improvement and are a classic example of evolution via allopolyploidization. In this study, we investigated chromosomes and subgenome transmission behavior in the newly formed allopolyploid of wheat group via multicolor Fluorescence in situ hybridization (mc-FISH), using pSc119.2, pTa535, and (GAA)7 as probe combinations, to enabled us to precisely identify individual chromosomes in 381 S3 and S4 generations plants derived from reciprocal crosses between Ae. ventricosa (DvDvNvNv) and T. turgidum (AABB). A higher rate of aneuploidy, constituting 66.04-86.41% individuals, was observed in these two early generations. Of the four constituent subgenomes, Dv showed the highest frequency of elimination, followed by Nv and B, while A was the most stable. In addition, structural chromosomal changes occurred ubiquitously in the selfed progenies of allopolyploids. Among the constituent subgenomes, B showed the highest number of aberrations. In terms of chromosomal dynamics, there was no significant association between the chromosomal behavior model and the cytoplasm, with the exception of chromosomal loss in the Dv subgenome. The chromosome loss frequency in the Dv subgenome was significantly higher in the T. turgidum × Ae. ventricosa cross than in the Ae. ventricosa × T. turgidum cross. This result indicates that, although the D subgenome showed great instability, allopolyploids containing D subgenome could probably be maintained after a certain hybridization in which the D subgenome donor was used as the maternal parent at its onset stage. Our findings provide valuable information pertaining to the behavior patterns of subgenomes during allopolyploidization. Moreover, the allopolyploids developed here could be used as potential resources for the genetic improvement of wheat.

Biochemical and genetic basis of cadmium biosorption by Enterobacter ludwigii LY6, isolated from industrial contaminated soil.

In this study, a cadmium-tolerant bacterium, Enterobacter ludwigii LY6, was isolated from cadmium-contaminated soil in Shifang, Sichuan province, China. The cadmium chloride removal rate of the strain LY6 with a treatment of 100 mg/L cadmium chloride reached 56.0%. Scanning electron microscopy showed that exopolysaccharides (EPS) might be the main means of cadmium adsorption by the strain. X-ray powder diffraction (XRD) and energy-dispersive X-ray spectroscopy (EDS) analyses indicated that cadmium sulfide nanoparticles formed on the surface of bacteria cultured in a medium containing 100 mg/L cadmium chloride. In addition, the expression of several genes increased with the increase of the cadmium concentration in the medium, including the multiple antibiotic resistance proteins marA and marR, and the cold shock protein CspA. GO functions, such as the redox activity, respiratory chain and transport functions, and KEGG pathways involved in "bacterial chemotaxis" and "terpenoid backbone biosynthesis" were found to be closely related to bacterial cadmium tolerance and biosorption. This is the first report that E. ludwigii can reduce sulfate to form cadmium sulfide nanoparticles under high concentration cadmium exposure. The genes related to cadmium tolerance identified in this study lay a foundation for the genetic breeding of cadmium-tolerant strains.