[Digital image in the practice of Cytology: a pilot study.]

Cytological study is a highly specialized type of laboratory analysis of the cellular composition of biological material and is to assess the morphological characteristics of cellular elements. The modern development of digital technologies is increasingly forming the interest of specialists to such a section as telepathology (digital pathology), which is a process of virtual microscopy with the transformation of classical cytological preparations into digital. Most morphologists currently use some forms of digital imaging, such as static images obtained by optical cameras mounted under a microscope. The development of more high quality image and resolution in the digital pathology promotes the use of telepathology, including telecitology in their daily work for training specialists, counselling of medications, monitoring the quality of diagnosis.

[Possibilities of cytological diagnosis of the nature of the exudate at the stage of emergency.]

Cytological diagnosis by effusions is currently the only reliable method of morphological verification of the diagnosis, it has prognostic significance and determines the choice of treatment strategy. At the same time, the variability of normal mesothelial cells causes significant difficulties in its differential diagnosis with reactive mesothelium, malignant mesothelioma and cancer metastasis, which requires additional analytical methods. A retrospective study of cytological preparations for 2017 was conducted, as well as the effectiveness of the use of fluorescent immunocytochemistry (FITZ) on the test system "biochip" in combination with a traditional cytological study was evaluated. During the period of November 2017 - July 2018, 46 exudates of serous cavities were studied, which showed that 9 patients (19.6%) were diagnosed with metastatic effusion, 31 (66.7%) patients had reactive exudate, suspicion of the malignant nature of serous fluid was expressed in 4 patients (8.7%), and 4.8% of persons (2 samples) failed to make an accurate diagnosis. After an additional FITZ study using the "Biochip" test system, the number of patients diagnosed with metastatic effusion increased to 7 (25.9%) due to a decrease in the percentage of cases of unspecified effusion. The combined use of traditional cytology and fluorescent immunocytochemistry in the diagnosis of effusion fluids at the stage of emergency medical care to the patient complements each other and contributes to a faster and more reliable diagnosis, as it allows to confirm the malignancy of the test material, and to assume the primary focus.

[Optical coherence tomography in surgical treatment of esophageal cancer].

The authors present an analysis of possibilities of optical coherence tomography in preoperative determination of the proximal interface of the tumor. Under investigation there were 24 patients (14 of them with squamous cell carcinoma and 10 with adenocarcinoma), with localization of the process not more than 5 cm the from z-line.

[Effect of ligand orientation on the redox potential of homologous proteins]. / Vliianie orientatsii ligandov na redoks-potentsial gomologichnykh belkov.

Using experimental g-values of homologous cytochromes isolated from the horse heart and bacterial cyt-c-551 from Pseudomonas aeruginosa, the electron energy difference of redox-orbital of Fe(III) ion of heme was calculated during reduction. Data are in a good accordance with experimental redox-potential values for these proteins. The model gives opportunity to predict redox-potential values for other homologous proteins.

[Heme-heme interactions in circular dichroism spectra]. / Proiavlenie gem-gem vzaimodeistviia v spektrakh krugovogo dikhroizma.

Using the theory of optical activity by Kirkwood and Tinoco, the CD spectra of the heme peptides in mono- and dimeric forms have been calculated according to the simple spectral model of heme of Simpson.