Composition, preparation methods, and applications of nanoniosomes as codelivery systems: a review of emerging therapies with emphasis on cancer.

Nanoniosome-based drug codelivery systems have become popular therapeutic instruments, demonstrating tremendous promise in cancer therapy, infection treatment, and other therapeutic domains. An emerging form of vesicular nanocarriers, niosomes are self-assembling vesicles composed of nonionic surfactants, along with cholesterol or other amphiphilic molecules. This comprehensive review focuses on how nanosystems may aid in making anticancer and antibacterial pharmaceuticals more stable and soluble. As malleable nanodelivery instruments, the composition, types, preparation procedures, and variables affecting the structure and stability of niosomes are extensively investigated. In addition, the advantages of dual niosomes for combination therapy and the administration of multiple medications simultaneously are highlighted. Along with categorizing niosomal drug delivery systems, a comprehensive analysis of various preparation techniques, including thin-layer injection, ether injection, and microfluidization, is provided. Dual niosomes for cancer treatment are discussed in detail regarding the codelivery of two medications and the codelivery of a drug with organic, plant-based bioactive compounds or gene agents. In addition, niogelosomes and metallic niosomal carriers for targeted distribution are discussed. The review also investigates the simultaneous delivery of bioactive substances and gene agents, including siRNA, microRNA, shRNA, lncRNA, and DNA. Additional sections discuss the use of dual niosomes for cutaneous drug delivery and treating leishmanial infections, Pseudomonas aeruginosa, and Mycobacterium tuberculosis. The study concludes by delineating the challenges and potential routes for nanoniosome-based pharmaceutical codelivery systems, which will be useful for nanomedicine practitioners and researchers.

Wound closure, angiogenesis and antibacterial behaviors of tetracalcium phosphate/hydroxyethyl cellulose/hyaluronic acid/gelatin composite dermal scaffolds.

Polymeric and tetracalcium phosphate (TTCP)-containing polymeric scaffolds were fabricated using a freeze-drying technique, with a homogenous solution of hydroxyethyl cellulose (HEC)/hyaluronic acid (HA)/gelatin (G) or suspension of 15 or 20% TTCP) particles in HEC/HA/G solution. The morphology, phase composition, chemical bands, and swelling behavior of the scaffold were determined. In vitro fibroblast cell viability and migration potential of the scaffolds were determined by MTT, live/dead staining, and scratch assay for wound healing. The in vivo chick embryo angiogenesis test was also carried out. Finally, the initial antibacterial activity of the scaffolds was determined using Staphylococcus aureus. The scaffolds exhibited an enormous porous structure in which the size of pores increased by the presence of TTCP particles. While the polymeric scaffold was amorphous, the formation of low crystalline hydroxyapatite phase and the initial TTCP particles was determined in the composition of TTCP-added scaffolds. TTCP increased swelling behavior of the polymeric scaffold in PBS. The results demonstrated that the amount of TTCP was a crucial factor in cell life. A high concentration of TTCP could restrict cell viability, although all the scaffolds were nontoxic. The scratch assessments determined better cell migration and wound closure in treating with TTCP-containing scaffolds so that after 24 h, a wound closure of 100% was observed. Furthermore, TTCP-incorporated scaffolds significantly improved the angiogenesis, in the chick embryo test. The presence of TTCP had a significant effect on reducing the bacterial activity and 20% TTCP-containing scaffold exhibited better antibacterial activity than the others.

3D printed chitosan/polycaprolactone scaffold for lung tissue engineering: hope to be useful for COVID-19 studies.

To prevent or reduce mortality from lung diseases, new biological materials and scaffolds are needed to conduct more accurate research and support lung tissue regeneration. On the other hand, the outbreak of the COVID-19 virus and its targeting of the human lung has caused many deaths worldwide. The main aim of this study was to provide a biologically and mechanically suitable 3D printed scaffold using chitosan/polycaprolactone bioink for lung tissue engineering. Design-Expert software was employed for studying various compositions for 3D printing. The selected scaffolds underwent physiochemical, biological and mechanical studies to evaluate if they are capable of MRC-5 cell line growth, proliferation, and migration. Based on the results, the average diameter of the chitosan/polycaprolactone strands was measured at 360 µm. Chitosan concentration controlled the printability, while changes in polycaprolactone content did not affect printability. The scaffolds showed excellent potential in swelling, degradation, and mechanical behavior, although they can be modified by adjusting the polycaprolactone content. The scaffolds also revealed notable cell adhesion, nontoxicity, low apoptosis, high proliferation, and cell biocompatibility in vitro. To sum up, scaffold 3 (chitosan/polycaprolactone ratio: 4 : 1) revealed better activity for MRC-5 cell culture. Thereby, this scaffold can be a good candidate for lung tissue engineering and may be applicable for more studies on the COVID-19 virus.