The effect of simultaneous exposure of human fibroblasts to fluoride and moderate intensity static magnetic fields.

Background: The combined effect of exposure to a static magnetic field (SMF) and potentially toxic agents is a crucial research area, mainly due to occupational and environmental exposure to these factors. The aim of this study was to evaluate the effect of the simultaneous exposure of human fibroblasts to fluoride and a SMF.Materials and methods: Control fibroblasts and fibroblasts that had been treated with fluoride were subjected to an SMF at a moderate induction (0.45, 0.55 and 0.65 T). The intracellular reactive oxygen species production, the concentration of malondialdehyde and the activities of superoxide dismutase and glutathione peroxidase were measured.Results: Our investigations revealed that a moderate SMF does not enhance the action of fluoride in inducing oxidative stress by generating free radicalsConclusions: A moderate SMF may be a factor that weakens the toxic action of fluoride, which is important for the health of individuals that are co-exposed to an SMF and fluoride ions (F-) from occupational and environmental sources.

Impact of fluoride and a static magnetic field on the gene expression that is associated with the antioxidant defense system of human fibroblasts.

Fluoride cytotoxicity has been associated with apoptosis, oxidative stress, general changes in DNA and RNA and protein biosynthesis, whereas the results of studies on the effect of SMF on antioxidant activity of cells are contradictory. Therefore, the aim of our study was to evaluate the simultaneous exposure of human cells to fluoride SMF that are generated by permanent magnets on the expression profile of the genes that are associated with the antioxidant defense system. Control fibroblasts and fibroblasts that had been treated with fluoride were subjected to the influence of SMF with a moderate induction. In order to achieve our aims, we applied modern molecular biology techniques such as the oligonucleotide microarray. Among the antioxidant defense genes, five (SOD1, PLK3, CLN8, XPA, HAO1), whose expression was significantly altered by the action of fluoride ions and the exposure to SMF were normalized their expression was identified. We showed that fluoride ions cause oxidative stress, whereas exposure to SMF with a moderate induction can suppress their effects by normalizing the expression of the genes that are altered by fluoride. Our research may explain the molecular mechanisms of the influence of fluoride and SMF that are generated by permanent magnets on cells.