Assuntos
Soros Imunes/imunologia , Neurônios/fisiologia , Caramujos/fisiologia , Potenciais de Ação , Hormônio Adrenocorticotrópico/imunologia , Animais , Anticorpos/fisiologia , Membrana Celular/imunologia , Membrana Celular/fisiologia , Eletrofisiologia , Neurônios/imunologia , Fragmentos de Peptídeos/imunologia , Sinaptossomos/imunologiaAssuntos
Encéfalo/citologia , Aprendizagem por Discriminação/fisiologia , Memória/fisiologia , Rememoração Mental/fisiologia , Neuroglia/fisiologia , Neurônios/fisiologia , Animais , Comportamento de Escolha/fisiologia , Condicionamento Psicológico/fisiologia , Feminino , Orientação/fisiologia , RatosRESUMO
The synthesis and degradation of rat brain synaptosomal proteins were studied in three groups of animals: trained the behaviour pattern in the maze, "pseudo-trained" and control. These processes were assessed from protein specific radioactivity after 1, 3 days and after 1, 3, 6 and 9 weeks following intraventricular injection of 14C-lysine. The experiments showed three fractions differing in overall values of half-life (T50). An increase in specific radioactivity of brain proteins of trained animals was revealed as compared to that in "pseudo-trained" and control rats. T50 was recorded to rise for slow-metabolizing fractions of synaptosomal proteins of trained rats. Participation of synaptosomal proteins in the mechanisms of long-term memory is discussed.
Assuntos
Córtex Cerebral/metabolismo , Aprendizagem/fisiologia , Proteínas do Tecido Nervoso/metabolismo , Animais , Córtex Cerebral/ultraestrutura , Lisina/metabolismo , Masculino , Ratos , Sinaptossomos/metabolismo , Fatores de TempoRESUMO
A method of obtaining antineuronal and antiglial immune sera is described. Data of the quantitative immunochemical analysis of the antigens of the neuronal and glial fractions of the rat brain are presented. The neurons proved to contain 4, and the glia--3 brain-specific proteins; one or two of them are common for these cell fractions.
Assuntos
Antígenos , Encéfalo/citologia , Neuroglia/imunologia , Neurônios/imunologia , Animais , Encéfalo/imunologia , Imunodifusão , RatosRESUMO
A method of electrophoresis in polyacrylamide gel was applied to the study of the RNA synthesis in the phenol nuclei and the cytoplasm of the cells of the higher portions of the brain in rats under normal conditions, during training of these animals in the motor-defence habit in a complicated labyrinth, and in active control (irregular presentation of motor-defence stimuli). Behaviouristic stimulation and an irregular presentation to the animals of the same stimuli as in the elaboration of habits, but not leading to their formation, activated the RNA synthesis in the phenol nuclei with sedimentation characteristics of 18S, 28S and over. Thy cytoplasmic RNA displayed an increase in the labeled precursor incorporation in the 18S region.
